EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | L-valine | enzyme induced 100fold by L-valine | Streptomyces virginiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | (NH4)2SO4 | enzyme is sensitive to high concentrations | Streptomyces virginiae | |
1.4.1.8 | Ag+ | 0.01 mM in assay buffer | Streptomyces virginiae | |
1.4.1.8 | Ca2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
1.4.1.8 | Cu2+ | 0.01 mM in assay buffer | Streptomyces virginiae | |
1.4.1.8 | Hg+ | - |
Streptomyces virginiae | |
1.4.1.8 | Hg2+ | - |
Streptomyces virginiae | |
1.4.1.8 | Mg2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
1.4.1.8 | additional information | no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil | Streptomyces virginiae | |
1.4.1.8 | p-chloromercuribenzoate | 0.01 mM in assay buffer | Streptomyces virginiae | |
1.4.1.8 | Urea | 3.5 M, complete and irreversible inactivation | Streptomyces virginiae | |
1.4.1.8 | Zn2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
1.4.1.23 | Ag+ | 0.1 mM, strong inhibition | Streptomyces virginiae | |
1.4.1.23 | Ca2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
1.4.1.23 | Cu2+ | 0.1 mM, strong inhibition | Streptomyces virginiae | |
1.4.1.23 | Hg+ | 0.1 mM, strong inhibition | Streptomyces virginiae | |
1.4.1.23 | Hg2+ | 0.1 mM, strong inhibition | Streptomyces virginiae | |
1.4.1.23 | Mg2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
1.4.1.23 | additional information | not inhibitory: EDTA, benzamidine, PMSF, all at 5 mM, or citrate, cobalt(II), azide, Li+ and Mn2+, at 1 mM | Streptomyces virginiae | |
1.4.1.23 | p-chloromercuribenzoate | 0.1 mM, strong inhibition | Streptomyces virginiae | |
1.4.1.23 | Zn2+ | 1 mM, partial inhibition | Streptomyces virginiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.8 | 0.074 | - |
NADH | - |
Streptomyces virginiae | |
1.4.1.8 | 0.13 | - |
2-oxobutyrate | - |
Streptomyces virginiae | |
1.4.1.8 | 0.18 | - |
NAD+ | - |
Streptomyces virginiae | |
1.4.1.8 | 0.48 | - |
2-oxovalerate | - |
Streptomyces virginiae | |
1.4.1.8 | 0.5 | - |
2-oxoisocaproate | - |
Streptomyces virginiae | |
1.4.1.8 | 0.81 | - |
2-oxoisovalerate | - |
Streptomyces virginiae | |
1.4.1.8 | 0.81 | - |
2-oxocaproate | - |
Streptomyces virginiae | |
1.4.1.8 | 1.3 | - |
L-valine | - |
Streptomyces virginiae | |
1.4.1.8 | 3.2 | - |
L-norvaline | - |
Streptomyces virginiae | |
1.4.1.8 | 4 | - |
L-leucine | - |
Streptomyces virginiae | |
1.4.1.8 | 10.8 | - |
L-isoleucine | - |
Streptomyces virginiae | |
1.4.1.8 | 12.3 | - |
pyruvate | - |
Streptomyces virginiae | |
1.4.1.8 | 19.6 | - |
L-2-aminobutyrate | - |
Streptomyces virginiae | |
1.4.1.8 | 30 | - |
L-norleucine | - |
Streptomyces virginiae | |
1.4.1.8 | 55 | - |
NH3 | - |
Streptomyces virginiae | |
1.4.1.23 | 0.13 | - |
2-oxobutanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 0.48 | - |
2-oxovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 0.5 | - |
2-Oxoisohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 0.81 | - |
2-oxoisovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 0.81 | - |
2-Oxohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 1.3 | - |
L-valine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 3.2 | - |
L-norvaline | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 4 | - |
L-leucine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 10.8 | - |
L-isoleucine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 12.3 | - |
pyruvate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 19.6 | - |
L-2-aminobutanoate | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 30 | - |
L-norleucine | pH 10.0, 21°C | Streptomyces virginiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 41200 | - |
2 * 41200, SDS-PAGE | Streptomyces virginiae |
1.4.1.8 | 88000 | - |
gel filtration | Streptomyces virginiae |
1.4.1.23 | 41000 | - |
2 * 41000, SDS-PAGE | Streptomyces virginiae |
1.4.1.23 | 88000 | - |
gel filtration | Streptomyces virginiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.8 | L-valine + NAD(P)+ + H2O | Streptomyces virginiae | first step in valine catabolism | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.8 | Streptomyces virginiae | - |
strains A 3823.5 and ATCC 15413 | - |
1.4.1.23 | Streptomyces virginiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.8 | 180fold purification | Streptomyces virginiae |
1.4.1.23 | - |
Streptomyces virginiae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.4.1.8 | L-valine + H2O + NADP+ = 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | kinetic mechanism | Streptomyces virginiae | |
1.4.1.23 | L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | the reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds to the enzyme first, followed by 2-oxoisovalerate and NH3, and valine is released first, followed by NAD+ | Streptomyces virginiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 19.6 | - |
- |
Streptomyces virginiae |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.8 | -70°C, 25 mM sodium phosphate buffer pH 7, equal volume of glycerol, 3 months, stable | Streptomyces virginiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Streptomyces virginiae | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | r | |
1.4.1.8 | L-alanine + H2O + NAD+ | - |
Streptomyces virginiae | pyruvate + NH3 + NADH | pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate | ? | |
1.4.1.8 | L-isoleucine + H2O + NAD+ | - |
Streptomyces virginiae | 3-methyl-2-oxopentanoate + NADH + NH3 | - |
r | |
1.4.1.8 | L-leucine + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxoisocaproate + NADH + NH3 | 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate | r | |
1.4.1.8 | L-norleucine + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxocaproate + NADH + NH3 | 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate | r | |
1.4.1.8 | L-norvaline + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxovalerate + NH3 + NADH | optimal chain length for reductive amination | r | |
1.4.1.8 | L-norvaline + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxovalerate + NH3 + NADH | 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces virginiae | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces virginiae | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces virginiae | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | first step in valine catabolism | Streptomyces virginiae | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.8 | additional information | NH3 is the sole substrate as amino donor | Streptomyces virginiae | additional information | reductive amination: sequential ordered ternary-binary mechanism | ? | |
1.4.1.8 | additional information | pro-S hydrogen at C-4' of NADH is transferred to substrate, B-stereospecific | Streptomyces virginiae | additional information | reductive amination: sequential ordered ternary-binary mechanism | ? | |
1.4.1.23 | 2-oxobutanoate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxohexanoate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-norleucine + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-leucine + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxoisovalerate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-valine + H2O + NAD+ | the 4'-pro-S hydrogen atom of NADH is transferred to the substrate in the reductive-amination reaction | r | |
1.4.1.23 | 2-oxovalerate + H2O + NAD+ | - |
Streptomyces virginiae | L-norvaline + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-isoleucine + H2O + NAD+ | - |
r | |
1.4.1.23 | L-2-aminobutanoate + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-alanine + H2O + NAD+ | - |
Streptomyces virginiae | pyruvate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-isoleucine + H2O + NAD+ | - |
Streptomyces virginiae | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-leucine + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-norleucine + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-norvaline + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-valine + H2O + NAD+ | - |
Streptomyces virginiae | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | pyruvate + NH3 + NADH + H+ | - |
Streptomyces virginiae | L-alanine + H2O + NAD+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.8 | dimer | 2 * 41200, SDS-PAGE | Streptomyces virginiae |
1.4.1.23 | dimer | 2 * 41000, SDS-PAGE | Streptomyces virginiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 21 | - |
assay at | Streptomyces virginiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.8 | 5.75 | - |
L-isoleucine | - |
Streptomyces virginiae | |
1.4.1.8 | 11.9 | - |
L-leucine | - |
Streptomyces virginiae | |
1.4.1.8 | 17.9 | - |
L-norleucine | - |
Streptomyces virginiae | |
1.4.1.8 | 18 | - |
L-norvaline | - |
Streptomyces virginiae | |
1.4.1.8 | 28.3 | - |
L-valine | - |
Streptomyces virginiae | |
1.4.1.8 | 71.9 | - |
2-oxoisocaproate | - |
Streptomyces virginiae | |
1.4.1.8 | 99 | - |
2-oxobutyrate | - |
Streptomyces virginiae | |
1.4.1.8 | 104 | - |
L-2-aminobutyrate | - |
Streptomyces virginiae | |
1.4.1.8 | 107 | - |
2-oxocaproate | - |
Streptomyces virginiae | |
1.4.1.8 | 114 | - |
pyruvate | - |
Streptomyces virginiae | |
1.4.1.8 | 164 | - |
2-oxovalerate | - |
Streptomyces virginiae | |
1.4.1.8 | 226 | - |
2-oxoisovalerate | - |
Streptomyces virginiae | |
1.4.1.23 | 5.8 | - |
L-isoleucine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 11.9 | - |
L-leucine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 17.9 | - |
L-norleucine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 18 | - |
L-norvaline | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 28.3 | - |
L-valine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 71.9 | - |
2-Oxoisohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 99 | - |
2-oxobutanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 104 | - |
L-2-aminobutanoate | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 107 | - |
2-Oxohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 114 | - |
pyruvate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 164 | - |
2-oxovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 226 | - |
2-oxoisovalerate | pH 9.5, 21°C | Streptomyces virginiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 9.5 | - |
reductive amination | Streptomyces virginiae |
1.4.1.8 | 10 | - |
oxidative deamination | Streptomyces virginiae |
1.4.1.23 | 9.5 | - |
reductive amination | Streptomyces virginiae |
1.4.1.23 | 10 | - |
oxidative deamination | Streptomyces virginiae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 10.5 | - |
above rates of reductive amination and oxidative deamination decrease dramatically | Streptomyces virginiae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 7 | - |
below markedly unstable | Streptomyces virginiae |
1.4.1.8 | 7.4 | - |
most stable | Streptomyces virginiae |
1.4.1.23 | 7 | - |
dramatic decrease in stability below | Streptomyces virginiae |
1.4.1.23 | 7.4 | - |
most stable at | Streptomyces virginiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | additional information | not with NADP+ and NADPH | Streptomyces virginiae | |
1.4.1.8 | NAD+ | NAD+ required, no use of NADP+ | Streptomyces virginiae | |
1.4.1.8 | NADH | NADH could not be replaced with NADPH for the reductive amination | Streptomyces virginiae | |
1.4.1.23 | additional information | no cofactor: NADP+ | Streptomyces virginiae | |
1.4.1.23 | NAD+ | - |
Streptomyces virginiae | |
1.4.1.23 | NADH | - |
Streptomyces virginiae |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.4.1.23 | Streptomyces virginiae | isoelectric focusing | - |
6.2 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.23 | 0.53 | - |
L-isoleucine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 0.6 | - |
L-norleucine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 3 | - |
L-leucine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 5.3 | - |
L-2-aminobutanoate | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 5.6 | - |
L-norvaline | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 9.3 | - |
pyruvate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 21.8 | - |
L-valine | pH 10.0, 21°C | Streptomyces virginiae | |
1.4.1.23 | 131 | - |
2-Oxohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 143 | - |
2-Oxoisohexanoate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 279 | - |
2-oxoisovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 340 | - |
2-oxovalerate | pH 9.5, 21°C | Streptomyces virginiae | |
1.4.1.23 | 740 | - |
2-oxobutanoate | pH 9.5, 21°C | Streptomyces virginiae |