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Literature summary extracted from

  • Priestley, N.D.; Robinson, J.A.
    Purification and catalytic properties of L-valine dehydrogenase from Streptomyces cinnamonensis (1989), Biochem. J., 261, 853-861.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.8 L-valine enzyme induced 100fold by L-valine Streptomyces virginiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.8 (NH4)2SO4 enzyme is sensitive to high concentrations Streptomyces virginiae
1.4.1.8 Ag+ 0.01 mM in assay buffer Streptomyces virginiae
1.4.1.8 Ca2+ 1 mM, partial inhibition Streptomyces virginiae
1.4.1.8 Cu2+ 0.01 mM in assay buffer Streptomyces virginiae
1.4.1.8 Hg+
-
Streptomyces virginiae
1.4.1.8 Hg2+
-
Streptomyces virginiae
1.4.1.8 Mg2+ 1 mM, partial inhibition Streptomyces virginiae
1.4.1.8 additional information no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil Streptomyces virginiae
1.4.1.8 p-chloromercuribenzoate 0.01 mM in assay buffer Streptomyces virginiae
1.4.1.8 Urea 3.5 M, complete and irreversible inactivation Streptomyces virginiae
1.4.1.8 Zn2+ 1 mM, partial inhibition Streptomyces virginiae
1.4.1.23 Ag+ 0.1 mM, strong inhibition Streptomyces virginiae
1.4.1.23 Ca2+ 1 mM, partial inhibition Streptomyces virginiae
1.4.1.23 Cu2+ 0.1 mM, strong inhibition Streptomyces virginiae
1.4.1.23 Hg+ 0.1 mM, strong inhibition Streptomyces virginiae
1.4.1.23 Hg2+ 0.1 mM, strong inhibition Streptomyces virginiae
1.4.1.23 Mg2+ 1 mM, partial inhibition Streptomyces virginiae
1.4.1.23 additional information not inhibitory: EDTA, benzamidine, PMSF, all at 5 mM, or citrate, cobalt(II), azide, Li+ and Mn2+, at 1 mM Streptomyces virginiae
1.4.1.23 p-chloromercuribenzoate 0.1 mM, strong inhibition Streptomyces virginiae
1.4.1.23 Zn2+ 1 mM, partial inhibition Streptomyces virginiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.8 0.074
-
NADH
-
Streptomyces virginiae
1.4.1.8 0.13
-
2-oxobutyrate
-
Streptomyces virginiae
1.4.1.8 0.18
-
NAD+
-
Streptomyces virginiae
1.4.1.8 0.48
-
2-oxovalerate
-
Streptomyces virginiae
1.4.1.8 0.5
-
2-oxoisocaproate
-
Streptomyces virginiae
1.4.1.8 0.81
-
2-oxoisovalerate
-
Streptomyces virginiae
1.4.1.8 0.81
-
2-oxocaproate
-
Streptomyces virginiae
1.4.1.8 1.3
-
L-valine
-
Streptomyces virginiae
1.4.1.8 3.2
-
L-norvaline
-
Streptomyces virginiae
1.4.1.8 4
-
L-leucine
-
Streptomyces virginiae
1.4.1.8 10.8
-
L-isoleucine
-
Streptomyces virginiae
1.4.1.8 12.3
-
pyruvate
-
Streptomyces virginiae
1.4.1.8 19.6
-
L-2-aminobutyrate
-
Streptomyces virginiae
1.4.1.8 30
-
L-norleucine
-
Streptomyces virginiae
1.4.1.8 55
-
NH3
-
Streptomyces virginiae
1.4.1.23 0.13
-
2-oxobutanoate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 0.48
-
2-oxovalerate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 0.5
-
2-Oxoisohexanoate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 0.81
-
2-oxoisovalerate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 0.81
-
2-Oxohexanoate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 1.3
-
L-valine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 3.2
-
L-norvaline pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 4
-
L-leucine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 10.8
-
L-isoleucine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 12.3
-
pyruvate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 19.6
-
L-2-aminobutanoate pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 30
-
L-norleucine pH 10.0, 21°C Streptomyces virginiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.8 41200
-
2 * 41200, SDS-PAGE Streptomyces virginiae
1.4.1.8 88000
-
gel filtration Streptomyces virginiae
1.4.1.23 41000
-
2 * 41000, SDS-PAGE Streptomyces virginiae
1.4.1.23 88000
-
gel filtration Streptomyces virginiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.8 L-valine + NAD(P)+ + H2O Streptomyces virginiae first step in valine catabolism 2-oxoisovalerate + NH3 + NAD(P)H
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.8 Streptomyces virginiae
-
strains A 3823.5 and ATCC 15413
-
1.4.1.23 Streptomyces virginiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.8 180fold purification Streptomyces virginiae
1.4.1.23
-
Streptomyces virginiae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.8 L-valine + H2O + NADP+ = 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ kinetic mechanism Streptomyces virginiae
1.4.1.23 L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+ the reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds to the enzyme first, followed by 2-oxoisovalerate and NH3, and valine is released first, followed by NAD+ Streptomyces virginiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.8 19.6
-
-
Streptomyces virginiae

Storage Stability

EC Number Storage Stability Organism
1.4.1.8 -70°C, 25 mM sodium phosphate buffer pH 7, equal volume of glycerol, 3 months, stable Streptomyces virginiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces virginiae 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
1.4.1.8 L-alanine + H2O + NAD+
-
Streptomyces virginiae pyruvate + NH3 + NADH pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate ?
1.4.1.8 L-isoleucine + H2O + NAD+
-
Streptomyces virginiae 3-methyl-2-oxopentanoate + NADH + NH3
-
r
1.4.1.8 L-leucine + H2O + NAD+
-
Streptomyces virginiae 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
1.4.1.8 L-norleucine + H2O + NAD+
-
Streptomyces virginiae 2-oxocaproate + NADH + NH3 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate r
1.4.1.8 L-norvaline + H2O + NAD+
-
Streptomyces virginiae 2-oxovalerate + NH3 + NADH optimal chain length for reductive amination r
1.4.1.8 L-norvaline + H2O + NAD+
-
Streptomyces virginiae 2-oxovalerate + NH3 + NADH 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces virginiae 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces virginiae 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces virginiae 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
1.4.1.8 L-valine + NAD(P)+ + H2O first step in valine catabolism Streptomyces virginiae 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 additional information NH3 is the sole substrate as amino donor Streptomyces virginiae additional information reductive amination: sequential ordered ternary-binary mechanism ?
1.4.1.8 additional information pro-S hydrogen at C-4' of NADH is transferred to substrate, B-stereospecific Streptomyces virginiae additional information reductive amination: sequential ordered ternary-binary mechanism ?
1.4.1.23 2-oxobutanoate + NH3 + NADH + H+
-
Streptomyces virginiae L-2-aminobutanoate + H2O + NAD+
-
r
1.4.1.23 2-oxohexanoate + NH3 + NADH + H+
-
Streptomyces virginiae L-norleucine + H2O + NAD+
-
r
1.4.1.23 2-oxoisohexanoate + NH3 + NADH + H+
-
Streptomyces virginiae L-leucine + H2O + NAD+
-
r
1.4.1.23 2-oxoisovalerate + NH3 + NADH + H+
-
Streptomyces virginiae L-valine + H2O + NAD+ the 4'-pro-S hydrogen atom of NADH is transferred to the substrate in the reductive-amination reaction r
1.4.1.23 2-oxovalerate + H2O + NAD+
-
Streptomyces virginiae L-norvaline + NH3 + NADH + H+
-
r
1.4.1.23 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Streptomyces virginiae L-isoleucine + H2O + NAD+
-
r
1.4.1.23 L-2-aminobutanoate + H2O + NAD+
-
Streptomyces virginiae 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-alanine + H2O + NAD+
-
Streptomyces virginiae pyruvate + NH3 + NADH + H+
-
r
1.4.1.23 L-isoleucine + H2O + NAD+
-
Streptomyces virginiae 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-leucine + H2O + NAD+
-
Streptomyces virginiae 2-oxoisohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norleucine + H2O + NAD+
-
Streptomyces virginiae 2-oxohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norvaline + H2O + NAD+
-
Streptomyces virginiae 2-oxovalerate + NH3 + NADH + H+
-
r
1.4.1.23 L-valine + H2O + NAD+
-
Streptomyces virginiae 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 pyruvate + NH3 + NADH + H+
-
Streptomyces virginiae L-alanine + H2O + NAD+
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.8 dimer 2 * 41200, SDS-PAGE Streptomyces virginiae
1.4.1.23 dimer 2 * 41000, SDS-PAGE Streptomyces virginiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.8 21
-
assay at Streptomyces virginiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.8 5.75
-
L-isoleucine
-
Streptomyces virginiae
1.4.1.8 11.9
-
L-leucine
-
Streptomyces virginiae
1.4.1.8 17.9
-
L-norleucine
-
Streptomyces virginiae
1.4.1.8 18
-
L-norvaline
-
Streptomyces virginiae
1.4.1.8 28.3
-
L-valine
-
Streptomyces virginiae
1.4.1.8 71.9
-
2-oxoisocaproate
-
Streptomyces virginiae
1.4.1.8 99
-
2-oxobutyrate
-
Streptomyces virginiae
1.4.1.8 104
-
L-2-aminobutyrate
-
Streptomyces virginiae
1.4.1.8 107
-
2-oxocaproate
-
Streptomyces virginiae
1.4.1.8 114
-
pyruvate
-
Streptomyces virginiae
1.4.1.8 164
-
2-oxovalerate
-
Streptomyces virginiae
1.4.1.8 226
-
2-oxoisovalerate
-
Streptomyces virginiae
1.4.1.23 5.8
-
L-isoleucine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 11.9
-
L-leucine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 17.9
-
L-norleucine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 18
-
L-norvaline pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 28.3
-
L-valine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 71.9
-
2-Oxoisohexanoate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 99
-
2-oxobutanoate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 104
-
L-2-aminobutanoate pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 107
-
2-Oxohexanoate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 114
-
pyruvate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 164
-
2-oxovalerate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 226
-
2-oxoisovalerate pH 9.5, 21°C Streptomyces virginiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.8 9.5
-
reductive amination Streptomyces virginiae
1.4.1.8 10
-
oxidative deamination Streptomyces virginiae
1.4.1.23 9.5
-
reductive amination Streptomyces virginiae
1.4.1.23 10
-
oxidative deamination Streptomyces virginiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.8 10.5
-
above rates of reductive amination and oxidative deamination decrease dramatically Streptomyces virginiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.8 7
-
below markedly unstable Streptomyces virginiae
1.4.1.8 7.4
-
most stable Streptomyces virginiae
1.4.1.23 7
-
dramatic decrease in stability below Streptomyces virginiae
1.4.1.23 7.4
-
most stable at Streptomyces virginiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.8 additional information not with NADP+ and NADPH Streptomyces virginiae
1.4.1.8 NAD+ NAD+ required, no use of NADP+ Streptomyces virginiae
1.4.1.8 NADH NADH could not be replaced with NADPH for the reductive amination Streptomyces virginiae
1.4.1.23 additional information no cofactor: NADP+ Streptomyces virginiae
1.4.1.23 NAD+
-
Streptomyces virginiae
1.4.1.23 NADH
-
Streptomyces virginiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.1.23 Streptomyces virginiae isoelectric focusing
-
6.2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.23 0.53
-
L-isoleucine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 0.6
-
L-norleucine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 3
-
L-leucine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 5.3
-
L-2-aminobutanoate pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 5.6
-
L-norvaline pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 9.3
-
pyruvate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 21.8
-
L-valine pH 10.0, 21°C Streptomyces virginiae
1.4.1.23 131
-
2-Oxohexanoate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 143
-
2-Oxoisohexanoate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 279
-
2-oxoisovalerate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 340
-
2-oxovalerate pH 9.5, 21°C Streptomyces virginiae
1.4.1.23 740
-
2-oxobutanoate pH 9.5, 21°C Streptomyces virginiae