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Literature summary extracted from

  • Navarrete, R.M.; Vara, J.A.; Hutchinson, C.R.
    Purification of an inducible L-valine dehydrogenase of Streptomyces coelicolor A3(2) (1990), J. Gen. Microbiol., 136, 273-281.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.8 glycerol stabilizes Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.8 (NH4)2SO4
-
Streptomyces coelicolor
1.4.1.8 L-valine substrate inhibition at higher concentrations than 20 mM Streptomyces coelicolor
1.4.1.23 L-valine substrate inhibition, noncompetitive Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.8 0.093
-
NADH
-
Streptomyces coelicolor
1.4.1.8 0.17
-
NAD+
-
Streptomyces coelicolor
1.4.1.8 0.6
-
2-oxoisovalerate
-
Streptomyces coelicolor
1.4.1.8 10
-
L-valine
-
Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.8 41000
-
2 * 41000, SDS-PAGE Streptomyces coelicolor
1.4.1.8 70000
-
gel filtration Streptomyces coelicolor
1.4.1.23 41000
-
2 * 41000, SDS-PAGE Streptomyces coelicolor
1.4.1.23 70000
-
gel filtration Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.8 L-valine + NAD(P)+ + H2O Streptomyces coelicolor metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O Streptomyces coelicolor first step in valine catabolism 2-oxoisovalerate + NH3 + NAD(P)H
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.8 Streptomyces coelicolor
-
-
-
1.4.1.23 Streptomyces coelicolor
-
-
-
1.4.1.23 Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.8 143fold purification Streptomyces coelicolor
1.4.1.23
-
Streptomyces coelicolor

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.23 L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+ sequential ordered mechanism with non-competitive inhibition by valine Streptomyces coelicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.8 0.7
-
L-alanine Streptomyces coelicolor
1.4.1.8 1.2
-
L-leucine Streptomyces coelicolor
1.4.1.8 3.8
-
L-isoleucine Streptomyces coelicolor
1.4.1.8 14
-
L-valine Streptomyces coelicolor
1.4.1.8 19
-
L-2-aminobutyrate Streptomyces coelicolor
1.4.1.23 14
-
pH 10.4, 30°C, substrate L-valine Streptomyces coelicolor
1.4.1.23 19
-
pH 10.4, 30°C, substrate 2-aminobutanoate Streptomyces coelicolor

Storage Stability

EC Number Storage Stability Organism
1.4.1.8 -80°C, 50 mM potassium phosphate pH 7.5, 10% v/v glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, 1 month, loss of specific activity Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ 130% of the activity with L-valine Streptomyces coelicolor 2-oxobutyrate + NADH + NH3
-
?
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces coelicolor 2-oxobutyrate + NADH + NH3
-
?
1.4.1.8 L-alanine + H2O + NAD+ 5% of the activity with L-valine Streptomyces coelicolor pyruvate + NH3 + NADH
-
r
1.4.1.8 L-cysteine + H2O + NAD+ 3% of the activity with L-valine Streptomyces coelicolor 3-mercapto-2-oxopropanoate + NH3 + NADH
-
?
1.4.1.8 L-isoleucine + H2O + NAD+ 28% of the activity with L-valine Streptomyces coelicolor 3-methyl-2-oxopentanoate + NADH + NH3
-
?
1.4.1.8 L-leucine + H2O + NAD+ 8% of the activity with L-valine Streptomyces coelicolor 2-oxoisocaproate + NADH + NH3
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O L-valine oxidized efficiently Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O L-valine oxidized efficiently Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O metabolic connection between valine catabolism and biosynthesis of macrolide or polyether antibiotics Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O first step in valine catabolism Streptomyces coelicolor 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor A3(2) 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces coelicolor A3(2) 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O L-valine oxidized efficiently Streptomyces coelicolor A3(2) 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O L-valine oxidized efficiently Streptomyces coelicolor A3(2) 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 additional information not: D-valine Streptomyces coelicolor additional information
-
?
1.4.1.8 additional information not: D-valine Streptomyces coelicolor A3(2) additional information
-
?
1.4.1.23 L-2-aminobutanoate + H2O + NAD+ 130% of the activity with L-valine Streptomyces coelicolor 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-alanine + H2O + NAD+ 3% of the activity with L-valine Streptomyces coelicolor pyruvate + NH3 + NADH + H+
-
r
1.4.1.23 L-isoleucine + H2O + NAD+ 28% of the activity with L-valine Streptomyces coelicolor 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-leucine + H2O + NAD+ 8% of the activity with L-valine Streptomyces coelicolor 2-oxoisohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-valine + H2O + NAD+
-
Streptomyces coelicolor 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.8 dimer 2 * 41000, SDS-PAGE Streptomyces coelicolor
1.4.1.23 dimer 2 * 41000, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
1.4.1.23 VDH
-
Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.8 9
-
reductive amination of 2-oxoisovalerate Streptomyces coelicolor
1.4.1.8 10.5
-
oxidative deamination of L-valine Streptomyces coelicolor
1.4.1.23 9
-
reductive amination Streptomyces coelicolor
1.4.1.23 10.5
-
oxidative deamination Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.8 8.5 9.5 pH 8.5: 40% loss of activity, pH 9.5: 10% loss of activity Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.8 additional information not with NADP+ and NADPH Streptomyces coelicolor
1.4.1.8 NAD+ NAD+ required, no use of NADP+ Streptomyces coelicolor
1.4.1.8 NADH NADH could not be replaced with NADPH for the reductive amination Streptomyces coelicolor
1.4.1.23 additional information no cofactor: NADP+ Streptomyces coelicolor
1.4.1.23 NAD+
-
Streptomyces coelicolor
1.4.1.23 NADH
-
Streptomyces coelicolor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.8 36.8
-
L-valine
-
Streptomyces coelicolor

Expression

EC Number Organism Comment Expression
1.4.1.23 Streptomyces coelicolor activity is repressed in the presence of glucose and NH4+ or glycerol and NH4+ or asparagine down
1.4.1.23 Streptomyces coelicolor activity is induced in presence of D- and L-valine up