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Literature summary extracted from

  • Vancura, A.; Vancurova, I.; Volc, J.; Fussey, S.P.M.; Flieger, M.; Neuzil, J.; Marsalek, J.; Behal, V.
    Valine dehydrogenase from Streptomyces fradiae: purification and properties (1988), J. Gen. Microbiol., 134, 3213-3219.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.8 2-mercaptoethanol presence of 2-mercaptoethanol or dithiothreitol at 10 mM required for maximal activity Streptomyces fradiae
1.4.1.8 dithiothreitol presence of 2-mercaptoethanol or dithiothreitol at 10 mM required for maximal activity Streptomyces fradiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.8 Hg2+ HgCl2: strong inhibition, partially abolished in presence of 2-mercaptoethanol Streptomyces fradiae
1.4.1.8 p-chloromercuribenzoate strong inhibition, partially abolished in presence of 2-mercaptoethanol Streptomyces fradiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.8 0.029
-
NAD+
-
Streptomyces fradiae
1.4.1.8 0.05
-
NADH
-
Streptomyces fradiae
1.4.1.8 0.67
-
L-leucine
-
Streptomyces fradiae
1.4.1.8 0.8
-
2-oxoisovalerate
-
Streptomyces fradiae
1.4.1.8 0.82
-
2-oxoisocaproate
-
Streptomyces fradiae
1.4.1.8 1
-
L-valine
-
Streptomyces fradiae
1.4.1.8 1
-
2-oxobutyrate
-
Streptomyces fradiae
1.4.1.8 1.18
-
L-isoleucine
-
Streptomyces fradiae
1.4.1.8 1.33
-
L-norvaline
-
Streptomyces fradiae
1.4.1.8 1.94
-
2-oxovalerate
-
Streptomyces fradiae
1.4.1.8 2.23
-
2-oxocaproate
-
Streptomyces fradiae
1.4.1.8 3.07
-
L-2-aminobutyrate
-
Streptomyces fradiae
1.4.1.8 3.33
-
2-oxo-3-methylvalerate
-
Streptomyces fradiae
1.4.1.8 6.68
-
L-norleucine
-
Streptomyces fradiae
1.4.1.8 22
-
NH4+
-
Streptomyces fradiae
1.4.1.8 25
-
pyruvate
-
Streptomyces fradiae
1.4.1.8 28.2
-
L-alanine
-
Streptomyces fradiae
1.4.1.23 0.67
-
L-leucine pH 10.6, 30°C Streptomyces fradiae
1.4.1.23 0.8
-
2-oxoisovalerate pH 8.8, 30°C Streptomyces fradiae
1.4.1.23 0.82
-
2-Oxoisohexanoate pH 8.8, 30°C Streptomyces fradiae
1.4.1.23 1
-
2-oxobutanoate pH 8.8, 30°C Streptomyces fradiae
1.4.1.23 1
-
L-valine pH 10.6, 30°C Streptomyces fradiae
1.4.1.23 1.18
-
L-isoleucine pH 10.6, 30°C Streptomyces fradiae
1.4.1.23 1.33
-
L-norvaline pH 10.6, 30°C Streptomyces fradiae
1.4.1.23 1.94
-
2-oxovalerate pH 8.8, 30°C Streptomyces fradiae
1.4.1.23 2.23
-
2-Oxohexanoate pH 8.8, 30°C Streptomyces fradiae
1.4.1.23 3.07
-
L-2-aminobutanoate pH 10.6, 30°C Streptomyces fradiae
1.4.1.23 3.33
-
3-methyl-2-oxopentanoate pH 8.8, 30°C Streptomyces fradiae
1.4.1.23 6.68
-
L-norleucine pH 10.6, 30°C Streptomyces fradiae
1.4.1.23 25
-
pyruvate pH 8.8, 30°C Streptomyces fradiae
1.4.1.23 28.2
-
L-alanine pH 10.6, 30°C Streptomyces fradiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.8 18000
-
12 * 18000, SDS-PAGE Streptomyces fradiae
1.4.1.8 215000
-
equilibrium ultracentrifugation Streptomyces fradiae
1.4.1.8 218000
-
gel filtration Streptomyces fradiae
1.4.1.23 18000
-
12 * 18000, SDS-PAGE Streptomyces fradiae
1.4.1.23 215000 218000 gel filtration and equilibrium ultracentrifugation Streptomyces fradiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.8 L-valine + NAD(P)+ + H2O Streptomyces fradiae regulatory enzyme involved in biosynthesis of n-butyrate, a building unit of the oligoketide antibiotic tylosin, NAD+ as natural cofactor for oxidative deamination 2-oxoisovalerate + NH3 + NAD(P)H
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.8 Streptomyces fradiae
-
-
-
1.4.1.8 Streptomyces fradiae 30/3
-
-
-
1.4.1.23 Streptomyces fradiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.8 1508fold purification Streptomyces fradiae
1.4.1.23
-
Streptomyces fradiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.8 additional information
-
-
Streptomyces fradiae

Storage Stability

EC Number Storage Stability Organism
1.4.1.8 -20°C, 0.2 M Tris-HCl buffer, pH 7.4, 3 months, no loss of activity Streptomyces fradiae
1.4.1.8 -20°C, 70% (NH4)2SO4 suspension in 0.2 M Tris-HCl buffer, 5 months, stable Streptomyces fradiae
1.4.1.8 4°C, 0.2 M Tris-HCl buffer, pH 7.4, 24 h, no loss of activity Streptomyces fradiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ 68.9% of the activity with L-valine Streptomyces fradiae 2-oxobutyrate + NADH + NH3 reductive amination: 78% of the activity with 2-oxoisovalerate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ 68.9% of the activity with L-valine Streptomyces fradiae 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces fradiae 2-oxobutyrate + NADH + NH3 reductive amination: 78% of the activity with 2-oxoisovalerate r
1.4.1.8 L-2-aminobutyrate + H2O + NAD+ L-2-aminobutyrate is identical with L-alpha-aminobutyrate Streptomyces fradiae 2-oxobutyrate + NADH + NH3 2-oxobutyrate is identical with alpha-ketobutyrate r
1.4.1.8 L-alanine + H2O + NAD+ 14.7% of the activity with L-valine Streptomyces fradiae pyruvate + NH3 + NADH pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate r
1.4.1.8 L-alanine + H2O + NAD+ 14.7% of the activity with L-valine Streptomyces fradiae pyruvate + NH3 + NADH reductive amination: 31.6% of the activity with 2-oxoisovalerate r
1.4.1.8 L-isoleucine + H2O + NAD+ 29.4% of the activity with L-valine Streptomyces fradiae 3-methyl-2-oxopentanoate + NADH + NH3 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate r
1.4.1.8 L-isoleucine + H2O + NAD+ 29.4% of the activity with L-valine Streptomyces fradiae 3-methyl-2-oxopentanoate + NADH + NH3 reductive amination: 23.2% of the activity with 2-oxoisovalerate r
1.4.1.8 L-leucine + H2O + NAD+ 24.5% of the activity with L-valine Streptomyces fradiae 2-oxoisocaproate + NADH + NH3 reductive amination: 20% of the activity with 2-oxoisovalerate r
1.4.1.8 L-leucine + H2O + NAD+ 24.5% of the activity with L-valine Streptomyces fradiae 2-oxoisocaproate + NADH + NH3 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate r
1.4.1.8 L-norleucine + H2O + NAD+ 51.5% of the activity with L-valine Streptomyces fradiae 2-oxocaproate + NADH + NH3 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate r
1.4.1.8 L-norleucine + H2O + NAD+ 51.5% of the activity with L-valine Streptomyces fradiae 2-oxocaproate + NADH + NH3 reductive amination: 43.8% of the activity with 2-oxoisovalerate r
1.4.1.8 L-norvaline + H2O + NAD+ 98% of the activity with L-valine Streptomyces fradiae 2-oxovalerate + NH3 + NADH reductive amination: 79.5% of the activity with 2-oxoisovalerate r
1.4.1.8 L-norvaline + H2O + NAD+ 98% of the activity with L-valine Streptomyces fradiae 2-oxovalerate + NH3 + NADH 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
1.4.1.8 L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
1.4.1.8 L-valine + NAD(P)+ + H2O regulatory enzyme involved in biosynthesis of n-butyrate, a building unit of the oligoketide antibiotic tylosin, NAD+ as natural cofactor for oxidative deamination Streptomyces fradiae 2-oxoisovalerate + NH3 + NAD(P)H
-
?
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.8 L-valine + NAD(P)+ + H2O NAD+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H reductive amination: preferred substrate r
1.4.1.8 L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H NADH r
1.4.1.8 L-valine + NAD(P)+ + H2O NADP+ Streptomyces fradiae 30/3 2-oxoisovalerate + NH3 + NAD(P)H 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate r
1.4.1.23 2-oxobutanoate + NH3 + NADH + H+ 78% of the activity with 2-oxoisovalerate Streptomyces fradiae L-2-aminobutanoate + H2O + NAD+
-
r
1.4.1.23 2-oxohexanoate + NH3 + NADH + H+ 43.8% of the activity with 2-oxoisovalerate Streptomyces fradiae L-norleucine + H2O + NAD+
-
r
1.4.1.23 2-oxoisohexanoate + NH3 + NADH + H+ 20% of the activity with 2-oxoisovalerate Streptomyces fradiae L-leucine + H2O + NAD+
-
r
1.4.1.23 2-oxoisovalerate + NH3 + NADH + H+
-
Streptomyces fradiae L-valine + H2O + NAD+
-
r
1.4.1.23 2-oxovalerate + H2O + NAD+ 79.5% of the activity with 2-oxoisovalerate Streptomyces fradiae L-norvaline + NH3 + NADH + H+
-
r
1.4.1.23 3-methyl-2-oxopentanoate + NH3 + NADH + H+ 23.2% of the activity with 2-oxoisovalerate Streptomyces fradiae L-isoleucine + H2O + NAD+
-
r
1.4.1.23 L-2-aminobutanoate + H2O + NAD+ 68.9% of the activity with L-valine Streptomyces fradiae 2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-alanine + H2O + NAD+ 14.7% of the activity with L-valine Streptomyces fradiae pyruvate + NH3 + NADH + H+
-
r
1.4.1.23 L-isoleucine + H2O + NAD+ 29.4% of the activity with L-valine Streptomyces fradiae 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-leucine + H2O + NAD+ 24.5% of the activity with L-valine Streptomyces fradiae 2-oxoisohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norleucine + H2O + NAD+ 51.5% of the activity with L-valine Streptomyces fradiae 2-oxohexanoate + NH3 + NADH + H+
-
r
1.4.1.23 L-norvaline + H2O + NAD+ 98% of the activity with L-valine Streptomyces fradiae 2-oxovalerate + NH3 + NADH + H+
-
r
1.4.1.23 L-valine + H2O + NAD+
-
Streptomyces fradiae 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r
1.4.1.23 additional information no substrates: D-valine, D-leucine, D-isoleucine, glycine, L-threonine, fialanine, L-serine, L-cysteine, L-methionine, L-glutamic acid, L-aspartic acid, L-asparagine, L-glutamine, L-lysine, L-phenylalanine, L-tyrosine, L-histidine and L-tryptophan as well as 2-oxoglutarate, oxalacetate, phenylpyruvate and glyoxalate Streptomyces fradiae ?
-
?
1.4.1.23 pyruvate + NH3 + NADH + H+ 31.6% of the activity with 2-oxoisovalerate Streptomyces fradiae L-alanine + H2O + NAD+
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.8 dodecamer 12 * 18000, SDS-PAGE Streptomyces fradiae
1.4.1.23 dodecamer 12 * 18000, SDS-PAGE Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.8 30
-
assay at Streptomyces fradiae
1.4.1.8 65
-
-
Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.8 8.7 8.9 reductive amination of 2-oxoisovalerate, in presence of 0.3 M Tris-HCl buffer Streptomyces fradiae
1.4.1.8 10.5 10.7 oxidative deamination of L-valine, in presence of 0.3 M glycine-KCl-KOH buffer Streptomyces fradiae
1.4.1.23 8.8
-
reductive amination Streptomyces fradiae
1.4.1.23 10.6
-
oxidative deamination Streptomyces fradiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.8 1,N6-etheno-NAD+ 64.4% of the activity with NAD+ Streptomyces fradiae
1.4.1.8 3-acetylpyridine-NAD+ 64.5% of the activity with NAD+ Streptomyces fradiae
1.4.1.8 Alpha-NAD+ 3.6% of the activity with NAD+ Streptomyces fradiae
1.4.1.8 deamino-NAD+ VDH2: 124% of the activity with NAD+ Streptomyces fradiae
1.4.1.8 deamino-NAD+ 93.9% of the activity with NAD+ Streptomyces fradiae
1.4.1.8 NAD+ natural cofactor for oxidative deamination Streptomyces fradiae
1.4.1.8 NADH NADH could not be replaced with NADPH for the reductive amination Streptomyces fradiae
1.4.1.8 NADP+ 10.8% of the activity with NAD+ Streptomyces fradiae
1.4.1.8 nicotinamide guanine dinucleotide 92.9% of the activity with NAD+ Streptomyces fradiae
1.4.1.23 1,N6-etheno-NAD+ 64.4% of the activity with NAD+ Streptomyces fradiae
1.4.1.23 3-acetylpyridine-NAD+ 64.5% of the activity with NAD+ Streptomyces fradiae
1.4.1.23 Alpha-NAD+ 3.6% of the activity with NAD+ Streptomyces fradiae
1.4.1.23 deamino-NAD+ 93.3% of the activity with NAD+ Streptomyces fradiae
1.4.1.23 additional information no cofactors: deamido-NAD+, 3-pyridinealdehyde-NAD+, thionicotinamide-NAD+ Streptomyces fradiae
1.4.1.23 NAD+
-
Streptomyces fradiae
1.4.1.23 NADH
-
Streptomyces fradiae
1.4.1.23 NADP+ 10.8% of the activity with NAD+ Streptomyces fradiae
1.4.1.23 nicotinamide guanine dinucleotide 92.9% of the activity with NAD+ Streptomyces fradiae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.1.23 Streptomyces fradiae isoelectric focusing
-
4.7