EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | 2-mercaptoethanol | presence of 2-mercaptoethanol or dithiothreitol at 10 mM required for maximal activity | Streptomyces fradiae | |
1.4.1.8 | dithiothreitol | presence of 2-mercaptoethanol or dithiothreitol at 10 mM required for maximal activity | Streptomyces fradiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | Hg2+ | HgCl2: strong inhibition, partially abolished in presence of 2-mercaptoethanol | Streptomyces fradiae | |
1.4.1.8 | p-chloromercuribenzoate | strong inhibition, partially abolished in presence of 2-mercaptoethanol | Streptomyces fradiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.8 | 0.029 | - |
NAD+ | - |
Streptomyces fradiae | |
1.4.1.8 | 0.05 | - |
NADH | - |
Streptomyces fradiae | |
1.4.1.8 | 0.67 | - |
L-leucine | - |
Streptomyces fradiae | |
1.4.1.8 | 0.8 | - |
2-oxoisovalerate | - |
Streptomyces fradiae | |
1.4.1.8 | 0.82 | - |
2-oxoisocaproate | - |
Streptomyces fradiae | |
1.4.1.8 | 1 | - |
L-valine | - |
Streptomyces fradiae | |
1.4.1.8 | 1 | - |
2-oxobutyrate | - |
Streptomyces fradiae | |
1.4.1.8 | 1.18 | - |
L-isoleucine | - |
Streptomyces fradiae | |
1.4.1.8 | 1.33 | - |
L-norvaline | - |
Streptomyces fradiae | |
1.4.1.8 | 1.94 | - |
2-oxovalerate | - |
Streptomyces fradiae | |
1.4.1.8 | 2.23 | - |
2-oxocaproate | - |
Streptomyces fradiae | |
1.4.1.8 | 3.07 | - |
L-2-aminobutyrate | - |
Streptomyces fradiae | |
1.4.1.8 | 3.33 | - |
2-oxo-3-methylvalerate | - |
Streptomyces fradiae | |
1.4.1.8 | 6.68 | - |
L-norleucine | - |
Streptomyces fradiae | |
1.4.1.8 | 22 | - |
NH4+ | - |
Streptomyces fradiae | |
1.4.1.8 | 25 | - |
pyruvate | - |
Streptomyces fradiae | |
1.4.1.8 | 28.2 | - |
L-alanine | - |
Streptomyces fradiae | |
1.4.1.23 | 0.67 | - |
L-leucine | pH 10.6, 30°C | Streptomyces fradiae | |
1.4.1.23 | 0.8 | - |
2-oxoisovalerate | pH 8.8, 30°C | Streptomyces fradiae | |
1.4.1.23 | 0.82 | - |
2-Oxoisohexanoate | pH 8.8, 30°C | Streptomyces fradiae | |
1.4.1.23 | 1 | - |
2-oxobutanoate | pH 8.8, 30°C | Streptomyces fradiae | |
1.4.1.23 | 1 | - |
L-valine | pH 10.6, 30°C | Streptomyces fradiae | |
1.4.1.23 | 1.18 | - |
L-isoleucine | pH 10.6, 30°C | Streptomyces fradiae | |
1.4.1.23 | 1.33 | - |
L-norvaline | pH 10.6, 30°C | Streptomyces fradiae | |
1.4.1.23 | 1.94 | - |
2-oxovalerate | pH 8.8, 30°C | Streptomyces fradiae | |
1.4.1.23 | 2.23 | - |
2-Oxohexanoate | pH 8.8, 30°C | Streptomyces fradiae | |
1.4.1.23 | 3.07 | - |
L-2-aminobutanoate | pH 10.6, 30°C | Streptomyces fradiae | |
1.4.1.23 | 3.33 | - |
3-methyl-2-oxopentanoate | pH 8.8, 30°C | Streptomyces fradiae | |
1.4.1.23 | 6.68 | - |
L-norleucine | pH 10.6, 30°C | Streptomyces fradiae | |
1.4.1.23 | 25 | - |
pyruvate | pH 8.8, 30°C | Streptomyces fradiae | |
1.4.1.23 | 28.2 | - |
L-alanine | pH 10.6, 30°C | Streptomyces fradiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 18000 | - |
12 * 18000, SDS-PAGE | Streptomyces fradiae |
1.4.1.8 | 215000 | - |
equilibrium ultracentrifugation | Streptomyces fradiae |
1.4.1.8 | 218000 | - |
gel filtration | Streptomyces fradiae |
1.4.1.23 | 18000 | - |
12 * 18000, SDS-PAGE | Streptomyces fradiae |
1.4.1.23 | 215000 | 218000 | gel filtration and equilibrium ultracentrifugation | Streptomyces fradiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.8 | L-valine + NAD(P)+ + H2O | Streptomyces fradiae | regulatory enzyme involved in biosynthesis of n-butyrate, a building unit of the oligoketide antibiotic tylosin, NAD+ as natural cofactor for oxidative deamination | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.8 | Streptomyces fradiae | - |
- |
- |
1.4.1.8 | Streptomyces fradiae 30/3 | - |
- |
- |
1.4.1.23 | Streptomyces fradiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.8 | 1508fold purification | Streptomyces fradiae |
1.4.1.23 | - |
Streptomyces fradiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | additional information | - |
- |
Streptomyces fradiae |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.8 | -20°C, 0.2 M Tris-HCl buffer, pH 7.4, 3 months, no loss of activity | Streptomyces fradiae |
1.4.1.8 | -20°C, 70% (NH4)2SO4 suspension in 0.2 M Tris-HCl buffer, 5 months, stable | Streptomyces fradiae |
1.4.1.8 | 4°C, 0.2 M Tris-HCl buffer, pH 7.4, 24 h, no loss of activity | Streptomyces fradiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | 68.9% of the activity with L-valine | Streptomyces fradiae | 2-oxobutyrate + NADH + NH3 | reductive amination: 78% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | 68.9% of the activity with L-valine | Streptomyces fradiae | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | r | |
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Streptomyces fradiae | 2-oxobutyrate + NADH + NH3 | reductive amination: 78% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Streptomyces fradiae | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | r | |
1.4.1.8 | L-alanine + H2O + NAD+ | 14.7% of the activity with L-valine | Streptomyces fradiae | pyruvate + NH3 + NADH | pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate | r | |
1.4.1.8 | L-alanine + H2O + NAD+ | 14.7% of the activity with L-valine | Streptomyces fradiae | pyruvate + NH3 + NADH | reductive amination: 31.6% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-isoleucine + H2O + NAD+ | 29.4% of the activity with L-valine | Streptomyces fradiae | 3-methyl-2-oxopentanoate + NADH + NH3 | 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate | r | |
1.4.1.8 | L-isoleucine + H2O + NAD+ | 29.4% of the activity with L-valine | Streptomyces fradiae | 3-methyl-2-oxopentanoate + NADH + NH3 | reductive amination: 23.2% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-leucine + H2O + NAD+ | 24.5% of the activity with L-valine | Streptomyces fradiae | 2-oxoisocaproate + NADH + NH3 | reductive amination: 20% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-leucine + H2O + NAD+ | 24.5% of the activity with L-valine | Streptomyces fradiae | 2-oxoisocaproate + NADH + NH3 | 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate | r | |
1.4.1.8 | L-norleucine + H2O + NAD+ | 51.5% of the activity with L-valine | Streptomyces fradiae | 2-oxocaproate + NADH + NH3 | 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate | r | |
1.4.1.8 | L-norleucine + H2O + NAD+ | 51.5% of the activity with L-valine | Streptomyces fradiae | 2-oxocaproate + NADH + NH3 | reductive amination: 43.8% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-norvaline + H2O + NAD+ | 98% of the activity with L-valine | Streptomyces fradiae | 2-oxovalerate + NH3 + NADH | reductive amination: 79.5% of the activity with 2-oxoisovalerate | r | |
1.4.1.8 | L-norvaline + H2O + NAD+ | 98% of the activity with L-valine | Streptomyces fradiae | 2-oxovalerate + NH3 + NADH | 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces fradiae | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces fradiae | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces fradiae | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NADP+ | Streptomyces fradiae | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NADP+ | Streptomyces fradiae | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NADP+ | Streptomyces fradiae | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | regulatory enzyme involved in biosynthesis of n-butyrate, a building unit of the oligoketide antibiotic tylosin, NAD+ as natural cofactor for oxidative deamination | Streptomyces fradiae | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces fradiae 30/3 | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces fradiae 30/3 | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces fradiae 30/3 | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NADP+ | Streptomyces fradiae 30/3 | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
1.4.1.8 | L-valine + NAD(P)+ + H2O | NADP+ | Streptomyces fradiae 30/3 | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
1.4.1.23 | 2-oxobutanoate + NH3 + NADH + H+ | 78% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxohexanoate + NH3 + NADH + H+ | 43.8% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-norleucine + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxoisohexanoate + NH3 + NADH + H+ | 20% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-leucine + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxoisovalerate + NH3 + NADH + H+ | - |
Streptomyces fradiae | L-valine + H2O + NAD+ | - |
r | |
1.4.1.23 | 2-oxovalerate + H2O + NAD+ | 79.5% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-norvaline + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | 23.2% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-isoleucine + H2O + NAD+ | - |
r | |
1.4.1.23 | L-2-aminobutanoate + H2O + NAD+ | 68.9% of the activity with L-valine | Streptomyces fradiae | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-alanine + H2O + NAD+ | 14.7% of the activity with L-valine | Streptomyces fradiae | pyruvate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-isoleucine + H2O + NAD+ | 29.4% of the activity with L-valine | Streptomyces fradiae | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-leucine + H2O + NAD+ | 24.5% of the activity with L-valine | Streptomyces fradiae | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-norleucine + H2O + NAD+ | 51.5% of the activity with L-valine | Streptomyces fradiae | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-norvaline + H2O + NAD+ | 98% of the activity with L-valine | Streptomyces fradiae | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | L-valine + H2O + NAD+ | - |
Streptomyces fradiae | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
1.4.1.23 | additional information | no substrates: D-valine, D-leucine, D-isoleucine, glycine, L-threonine, fialanine, L-serine, L-cysteine, L-methionine, L-glutamic acid, L-aspartic acid, L-asparagine, L-glutamine, L-lysine, L-phenylalanine, L-tyrosine, L-histidine and L-tryptophan as well as 2-oxoglutarate, oxalacetate, phenylpyruvate and glyoxalate | Streptomyces fradiae | ? | - |
? | |
1.4.1.23 | pyruvate + NH3 + NADH + H+ | 31.6% of the activity with 2-oxoisovalerate | Streptomyces fradiae | L-alanine + H2O + NAD+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.8 | dodecamer | 12 * 18000, SDS-PAGE | Streptomyces fradiae |
1.4.1.23 | dodecamer | 12 * 18000, SDS-PAGE | Streptomyces fradiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 30 | - |
assay at | Streptomyces fradiae |
1.4.1.8 | 65 | - |
- |
Streptomyces fradiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.8 | 8.7 | 8.9 | reductive amination of 2-oxoisovalerate, in presence of 0.3 M Tris-HCl buffer | Streptomyces fradiae |
1.4.1.8 | 10.5 | 10.7 | oxidative deamination of L-valine, in presence of 0.3 M glycine-KCl-KOH buffer | Streptomyces fradiae |
1.4.1.23 | 8.8 | - |
reductive amination | Streptomyces fradiae |
1.4.1.23 | 10.6 | - |
oxidative deamination | Streptomyces fradiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.8 | 1,N6-etheno-NAD+ | 64.4% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.8 | 3-acetylpyridine-NAD+ | 64.5% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.8 | Alpha-NAD+ | 3.6% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.8 | deamino-NAD+ | VDH2: 124% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.8 | deamino-NAD+ | 93.9% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.8 | NAD+ | natural cofactor for oxidative deamination | Streptomyces fradiae | |
1.4.1.8 | NADH | NADH could not be replaced with NADPH for the reductive amination | Streptomyces fradiae | |
1.4.1.8 | NADP+ | 10.8% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.8 | nicotinamide guanine dinucleotide | 92.9% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.23 | 1,N6-etheno-NAD+ | 64.4% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.23 | 3-acetylpyridine-NAD+ | 64.5% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.23 | Alpha-NAD+ | 3.6% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.23 | deamino-NAD+ | 93.3% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.23 | additional information | no cofactors: deamido-NAD+, 3-pyridinealdehyde-NAD+, thionicotinamide-NAD+ | Streptomyces fradiae | |
1.4.1.23 | NAD+ | - |
Streptomyces fradiae | |
1.4.1.23 | NADH | - |
Streptomyces fradiae | |
1.4.1.23 | NADP+ | 10.8% of the activity with NAD+ | Streptomyces fradiae | |
1.4.1.23 | nicotinamide guanine dinucleotide | 92.9% of the activity with NAD+ | Streptomyces fradiae |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.4.1.23 | Streptomyces fradiae | isoelectric focusing | - |
4.7 |