EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | ADP | at 0.3 mM 40% activation | Proteus inconstans | |
1.4.1.4 | ATP | at 0.3 mM, 60% activation of reductive amination | Proteus inconstans |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.1.4 | dialysis against ammonium sulfate solution | Proteus inconstans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | ADP | at 0.3 mM, 22% inhibition of oxidative deamination | Proteus inconstans | |
1.4.1.4 | AMP | at 0.3 mM 33% inhibition of oxidative deamination | Proteus inconstans | |
1.4.1.4 | Hg2+ | at 1 mM, 100% activity loss | Proteus inconstans | |
1.4.1.4 | iodoacetamide | at 4 mM, complete inactivation | Proteus inconstans | |
1.4.1.4 | N-ethylmaleimide | at 0.8 mM, 44% inhibition | Proteus inconstans | |
1.4.1.4 | p-chloromercuriphenyl sulfonate | inactivetes, can be reversed by addition of cysteine | Proteus inconstans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.4 | 0.003 | - |
NADPH | reductive amination | Proteus inconstans | |
1.4.1.4 | 0.023 | - |
NADP+ | oxidative deamination | Proteus inconstans | |
1.4.1.4 | 0.37 | - |
NH4+ | reductive amination | Proteus inconstans | |
1.4.1.4 | 2.3 | - |
L-glutamate | oxidative deamination | Proteus inconstans | |
1.4.1.4 | 3.7 | - |
L-glutamate | reductive amination | Proteus inconstans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 40000 | - |
x * 40000, SDS-PAGE | Proteus inconstans |
1.4.1.4 | 400000 | - |
gel filtration | Proteus inconstans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | L-glutamate + NADP+ + H2O | Proteus inconstans | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.4 | Proteus inconstans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.4 | - |
Proteus inconstans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 231 | - |
oxidative deamination at 25°C | Proteus inconstans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | 2-oxoglutarate + NH4+ + NADPH | - |
Proteus inconstans | L-glutamate + NADP+ | - |
? | |
1.4.1.4 | L-glutamate + NADP+ + H2O | - |
Proteus inconstans | 2-oxoglutarate + NADPH + NH3 | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.4 | ? | x * 40000, SDS-PAGE | Proteus inconstans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 40 | - |
- |
Proteus inconstans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 50 | - |
irreversible heat inactivation | Proteus inconstans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 8.5 | - |
reductive amination | Proteus inconstans |
1.4.1.4 | 10 | - |
oxidative deamination | Proteus inconstans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 6 | 9 | unstable below pH 5.0 or above pH 10.0 | Proteus inconstans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | NADP+ | - |
Proteus inconstans | |
1.4.1.4 | NADPH | - |
Proteus inconstans |