EC Number | General Stability | Organism |
---|---|---|
1.4.1.4 | unstable when frozen at -20°C after a few h even with addition of 50% v/v glycerol | Phormidium laminosum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | Glutaric acid | at 20 mM, 25% inhibition | Phormidium laminosum | |
1.4.1.4 | L-Glutamic acid | at 20 mM 25% inhibition | Phormidium laminosum | |
1.4.1.4 | L-tryptophan | at 20 mM, 15% inhibition | Phormidium laminosum | |
1.4.1.4 | Urea | no activity at 6 mM | Phormidium laminosum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.4 | 0.064 | - |
NADPH | reductive amination | Phormidium laminosum | |
1.4.1.4 | 1.25 | - |
2-oxoglutarate | reductive amination | Phormidium laminosum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 280000 | - |
gel filtration | Phormidium laminosum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | L-glutamate + NADP+ + H2O | Phormidium laminosum | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.4 | Phormidium laminosum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.4 | - |
Phormidium laminosum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 32.9 | - |
reductive amination at 30°C | Phormidium laminosum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.4 | L-glutamate + NADP+ + H2O | - |
Phormidium laminosum | 2-oxoglutarate + NADPH + NH3 | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 60 | - |
reductive amination | Phormidium laminosum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 72 | - |
complete inactivation | Phormidium laminosum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.4 | 7.5 | - |
reductive amination in potassium phosphate buffer | Phormidium laminosum |
1.4.1.4 | 8 | - |
reductive amination in Tris hydrochloride buffer | Phormidium laminosum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.4 | NADP+ | - |
Phormidium laminosum | |
1.4.1.4 | NADPH | - |
Phormidium laminosum |