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Literature summary extracted from

  • Hudson, R.C.; Ruttersmith, L.D.; Daniel, R.M.
    Glutamate dehydrogenase from the extremely thermophilic archaebacterial isolate AN1 (1993), Biochim. Biophys. Acta, 1202, 244-250.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.4 CaCl2 at 5 mM, 135% activation, oxidative deamination Thermococcus sp.
1.4.1.4 MgCl2 at 5 mM, 104% activity, oxidative deamination Thermococcus sp.
1.4.1.4 MnCl2 at 5 mM, 250% activity, oxidative deamination Thermococcus sp.

General Stability

EC Number General Stability Organism
1.4.1.4 10 mM MOPS-NaOH pH 7.1 and 0.3-0.4 M NaCl is the best buffer Thermococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 HgCl2 at 1 mM, no activity, oxidative deamination Thermococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.038
-
 NADP+ oxidative deamination at 80°C Thermococcus sp.
1.4.1.4 0.066
-
 NADPH reductive amination at 80°C Thermococcus sp.
1.4.1.4 1.7
-
 2-oxoglutarate reductive amination at 80°C Thermococcus sp.
1.4.1.4 9.12
-
 L-glutamate oxidative deamination at 80°C Thermococcus sp.
1.4.1.4 15.5
-
 NH4+ reductive amination at 80°C Thermococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 47000
-
 alpha4, 4 * 47000, SDS-PAGE Thermococcus sp.
1.4.1.4 204000
-
 gel filtration Thermococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Thermococcus sp.
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Thermococcus sp.
-
 isolate AN1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Thermococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 464
-
 oxidative deamination at 80°C and pH 7.54 Thermococcus sp.
1.4.1.4 2940
-
 reductive amination at 80°C and pH 7.54 Thermococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Thermococcus sp. L-glutamate + NADP+
-
 ?
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Thermococcus sp. 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homotetramer alpha4, 4 * 47000, SDS-PAGE Thermococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 90
-
 50% activity after 12.5 h in 0.1 M NaH2PO4/Na2HPO4 Thermococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8.3
-
 reductive amination and oxidative deamination Thermococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Thermococcus sp.
1.4.1.4 NADPH
-
 Thermococcus sp.