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Literature summary extracted from

  • Ohshima, T.; Nishida, N.
    Purification and properties of extremely thermostable glutamate dehydrogenase from two hyperthermophilic archaebacteria, Pyrococcus woesei and Pyrococcus furiosus (1993), Biosci. Biotechnol. Biochem., 57, 945-951.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.4 KCl at concentrations below 1 mM Pyrococcus woesei
1.4.1.4 KCl at concentrations below 2 mM Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 AlCl3 at 1 mM, 30% inhibition Pyrococcus furiosus
1.4.1.4 AlCl3 at 1 mM, 21% inhibition Pyrococcus woesei
1.4.1.4 HgCl2 at 1 mM, 64% inhibition Pyrococcus furiosus
1.4.1.4 HgCl2 at 1 mM, 45% inhibition Pyrococcus woesei
1.4.1.4 MnCl2 at 1 mM, 63% inhibition Pyrococcus furiosus
1.4.1.4 MnCl2 at 1 mM, 20% inhibition Pyrococcus woesei
1.4.1.4 Pb(CH3COO)2 at 1 mM, 59% inhibition Pyrococcus furiosus
1.4.1.4 Pb(CH3COO)2 at 1 mM, 64% inhibition Pyrococcus woesei
1.4.1.4 pyridoxal 5'-phosphate at 1 mM, 35% inhibition Pyrococcus furiosus
1.4.1.4 pyridoxal 5'-phosphate at 1 mM, 45% inhibition Pyrococcus woesei
1.4.1.4 Urea inactivation with 2 mM urea Pyrococcus furiosus
1.4.1.4 Urea fully active at 20°C in 8 mM urea Pyrococcus woesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 47000
-
alpha6, 6 * 47000, SDS-PAGE Pyrococcus furiosus
1.4.1.4 47000
-
alpha6, 6 * 47000, SDS-PAGE Pyrococcus woesei
1.4.1.4 300000
-
gel filtration Pyrococcus furiosus
1.4.1.4 300000
-
gel filtration Pyrococcus woesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Pyrococcus furiosus
-
2-oxoglutarate + NADPH + NH3
-
r
1.4.1.4 L-glutamate + NADP+ + H2O Pyrococcus woesei
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Pyrococcus furiosus
-
strain DSM 3638
-
1.4.1.4 Pyrococcus woesei
-
strain DSM 3773
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 10.3
-
oxidative deamination at 50°C and pH 8.2 Pyrococcus furiosus
1.4.1.4 24.3
-
oxidative deamination at 50°C and pH 8.2 Pyrococcus woesei

Storage Stability

EC Number Storage Stability Organism
1.4.1.4 4°C, 10 mM potassium phosphate buffer, pH 7.2, 0.1 mM EDTA, 0.1 mM DTT, 5 months, no activity loss Pyrococcus furiosus
1.4.1.4 4°C, 10 mM potassium phosphate buffer, pH 7.2, 0.1 mM EDTA, 0.1 mM DTT, 5 months, no activity loss Pyrococcus woesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O
-
Pyrococcus furiosus 2-oxoglutarate + NADPH + NH3
-
r
1.4.1.4 L-glutamate + NADP+ + H2O
-
Pyrococcus woesei 2-oxoglutarate + NADPH + NH3
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homohexamer alpha6, 6 * 47000, SDS-PAGE Pyrococcus furiosus
1.4.1.4 homohexamer alpha6, 6 * 47000, SDS-PAGE Pyrococcus woesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 100
-
oxidative deamination Pyrococcus furiosus
1.4.1.4 100
-
oxidative deamination Pyrococcus woesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 105
-
full activity after 10 min, 50% activity after 90 min Pyrococcus furiosus
1.4.1.4 105
-
full activity after 10 min, 50% activity after 90 min Pyrococcus woesei
1.4.1.4 115
-
inactivation after 10 min Pyrococcus furiosus
1.4.1.4 115
-
inactivation after 10 min Pyrococcus woesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 7.4
-
reductive amination at 50°C Pyrococcus furiosus
1.4.1.4 7.4
-
reductive amination at 50°C Pyrococcus woesei
1.4.1.4 8.2
-
oxidative deamination at 50°C Pyrococcus furiosus
1.4.1.4 8.2
-
oxidative deamination at 50°C Pyrococcus woesei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.4 4 10
-
Pyrococcus furiosus
1.4.1.4 4 10
-
Pyrococcus woesei

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
Pyrococcus furiosus
1.4.1.4 NADP+
-
Pyrococcus woesei
1.4.1.4 NADPH
-
Pyrococcus furiosus
1.4.1.4 NADPH
-
Pyrococcus woesei