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Literature summary extracted from

  • Ferrer, J.; Perez-Pomares, F.; Bonete, M.J.
    NADP-glutamate dehydrogenase from the halophilic archaeon Haloferax mediterranei: enzyme purification, N-terminal sequence and stability studies (1996), FEMS Microbiol. Lett., 141, 59-63.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.4 KCl optimal activity at 1-1.5 M Haloferax mediterranei
1.4.1.4 NaCl optimal activity at 1-1.5 M Haloferax mediterranei

General Stability

EC Number General Stability Organism
1.4.1.4 in the presence of 4 M NaCl the purified enzyme remains fully active at room temperature for months. The stability is rapidly lost upon lowering the salt concentration Haloferax mediterranei
1.4.1.4 NaCl and KCl markedly increase the thermostability Haloferax mediterranei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.018
-
 NADPH reductive amination at 25°C Haloferax mediterranei
1.4.1.4 0.018
-
 2-oxoglutarate pH 8.5, 40°C Haloferax mediterranei
1.4.1.4 0.34
-
 2-oxoglutarate reductive amination at 25°C Haloferax mediterranei
1.4.1.4 0.34
-
 NH3 pH 8.5, 40°C Haloferax mediterranei
1.4.1.4 4.2
-
 NH4+ reductive amination at 25°C Haloferax mediterranei
1.4.1.4 4.2
-
 NADPH pH 8.5, 40°C Haloferax mediterranei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.4 KCl stimulating effect of KCl is slightly higher than the effect of identical concentrations of NaCl Haloferax mediterranei
1.4.1.4 NaCl stimulating effect of KCl is slightly higher than the effect of identical concentrations of NaCl Haloferax mediterranei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 48000
-
 alpha6, 6 * 48000, CTAB-PAGE Haloferax mediterranei
1.4.1.4 55000
-
 alpha6, 6 * 55000, SDS-PAGE Haloferax mediterranei
1.4.1.4 55000
-
 6 * 55000, SDS-PAGE Haloferax mediterranei
1.4.1.4 320000
-
 gel filtration Haloferax mediterranei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Haloferax mediterranei
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Haloferax mediterranei DSM 1411
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.4.1.4 Glycerol stabilized by glycerol in absence of salt Haloferax mediterranei

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Haloferax mediterranei
-
-
-
1.4.1.4 Haloferax mediterranei DSM 1411
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Haloferax mediterranei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 60
-
 reductive amination at 40°C Haloferax mediterranei
1.4.1.4 60
-
 pH 8.5, 40°C Haloferax mediterranei

Storage Stability

EC Number Storage Stability Organism
1.4.1.4 25°C, 20% v/v glycerol, 476 h, 50% activity Haloferax mediterranei
1.4.1.4 fully active at 4 M concentration NaCl at room temperature for months Haloferax mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH3 + NADPH + H+
-
 Haloferax mediterranei L-glutamate + H2O + NADP+
-
 ?
1.4.1.4 2-oxoglutarate + NH3 + NADPH + H+
-
 Haloferax mediterranei DSM 1411 L-glutamate + H2O + NADP+
-
 ?
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Haloferax mediterranei L-glutamate + NADP+
-
 ?
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Haloferax mediterranei DSM 1411 L-glutamate + NADP+
-
 ?
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Haloferax mediterranei 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Haloferax mediterranei DSM 1411 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 hexamer 6 * 55000, SDS-PAGE Haloferax mediterranei
1.4.1.4 homohexamer alpha6, 6 * 55000, SDS-PAGE Haloferax mediterranei
1.4.1.4 homohexamer alpha6, 6 * 48000, CTAB-PAGE Haloferax mediterranei

Synonyms

EC Number Synonyms Comment Organism
1.4.1.4 NADP(H)-specific glutamate dehydrogenase
-
 Haloferax mediterranei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 40
-
 assay at Haloferax mediterranei
1.4.1.4 60
-
-
 Haloferax mediterranei
1.4.1.4 60
-
 reductive amination Haloferax mediterranei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 additional information
-
 the thermostability decreases significantly as the salt concentration is lowered Haloferax mediterranei
1.4.1.4 67
-
 t1/2 in presence of 2 M KCl is about 27 min, while in 4 M KCl the t1/2 is 70 h Haloferax mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8.5
-
-
 Haloferax mediterranei
1.4.1.4 8.5
-
 reductive amination Haloferax mediterranei

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Haloferax mediterranei
1.4.1.4 NADPH
-
 Haloferax mediterranei