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Literature summary extracted from

  • Steen, I.H.; Hvoslef, H.; Lien, T.; Birkeland, N.K.
    Isocitrate dehydrogenase, malate dehydrogenase, and glutamate dehydrogenase from Archaeoglobus fulgidus (2001), Methods Enzymol., 331, 13-26.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.4 K2HPO4 at 0.15 M three to fourfold stimulation of activity Archaeoglobus fulgidus
1.4.1.4 KCl at 0.2 M three to fourfold stimulation of activity Archaeoglobus fulgidus
1.4.1.4 NaCl at 0.2 M three to fourfold stimulation of activity Archaeoglobus fulgidus

General Stability

EC Number General Stability Organism
1.4.1.4 potassium phosphate enhances the thermostability Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.02
-
 NADPH reductive amination at 60°C Archaeoglobus fulgidus
1.4.1.4 0.06
-
 NADP+ oxidative deamination at 60°C Archaeoglobus fulgidus
1.4.1.4 3.9
-
 L-glutamate oxidative deamination at 60°C Archaeoglobus fulgidus
1.4.1.4 4
-
 NH3 reductive amination at 60°C Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 47000
-
 alpha6, 6 * 47000, SDS-PAGE Archaeoglobus fulgidus
1.4.1.4 263000
-
 gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Archaeoglobus fulgidus
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 additional information Archaeoglobus fulgidus glutamate dehydrogenase functions physiologically for the synthesis of L-glutamate from 2-oxoglutarate and ammonia ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Archaeoglobus fulgidus
-
 strain DSM 8774
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4 under semianoxically conditions Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Archaeoglobus fulgidus L-glutamate + H2O + NAD+
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Archaeoglobus fulgidus 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 additional information glutamate dehydrogenase functions physiologically for the synthesis of L-glutamate from 2-oxoglutarate and ammonia Archaeoglobus fulgidus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homohexamer alpha6, 6 * 47000, SDS-PAGE Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 95
-
 reductive amination Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 90
-
 fully active after 5 h Archaeoglobus fulgidus
1.4.1.4 100
-
 50% activity after 140 min Archaeoglobus fulgidus
1.4.1.4 105
-
 50% activity after 20 min Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8
-
 oxidative deamination at 60°C Archaeoglobus fulgidus
1.4.1.4 8.4
-
 reductive amination at 60°C Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Archaeoglobus fulgidus
1.4.1.4 NADPH
-
 Archaeoglobus fulgidus