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Literature summary extracted from

  • Bhuiya, M.W.; Sakuraba, H.; Ohshima, T.
    Temperature dependence of kinetic parameters for hyperthermophilic glutamate dehydrogenase from Aeropyrum pernix K1 (2002), Biosci. Biotechnol. Biochem., 66, 873-876.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.039
-
 NADP+ oxidative deamination Aeropyrum pernix K1
1.4.1.4 0.075
-
 NADPH reductive amination at 70°C Aeropyrum pernix K1
1.4.1.4 2
-
 2-oxoglutarate reductive amination at 0.6 M NH4Cl Aeropyrum pernix K1
1.4.1.4 9
-
 2-oxoglutarate reductive amination at 1 M NH4Cl Aeropyrum pernix K1
1.4.1.4 119
-
 NH3 reductive amination at 70°C Aeropyrum pernix K1
1.4.1.4 225
-
 L-glutamate oxidative deamination Aeropyrum pernix K1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Aeropyrum pernix K1
-
 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O Aeropyrum pernix K1 JCM 9820
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Aeropyrum pernix K1
-
-
-
1.4.1.4 Aeropyrum pernix K1 JCM 9820
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Aeropyrum pernix K1 L-glutamate + H2O + NAD+
-
 r
1.4.1.4 2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Aeropyrum pernix K1 JCM 9820 L-glutamate + H2O + NAD+
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Aeropyrum pernix K1 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Aeropyrum pernix K1 2-oxoglutarate + NADPH + NH3 biphasic kinetic behavior for ammonia r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Aeropyrum pernix K1 JCM 9820 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Aeropyrum pernix K1 JCM 9820 2-oxoglutarate + NADPH + NH3 biphasic kinetic behavior for ammonia r

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.4 additional information
-
 no temperature dependence Aeropyrum pernix K1

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Aeropyrum pernix K1
1.4.1.4 NADPH
-
 Aeropyrum pernix K1