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Literature summary extracted from

  • Fang, J.; Hsu, B.Y.L.; MacMullen, C.M.; Poncz, M.; Smith, T.J.; Stanley, C.A.
    Expression, purification and characterization of human glutamate dehydrogenase (GDH) allosteric regulatory mutations (2002), Biochem. J., 363, 81-87.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.3 ADP no activation of R463A mutant enzyme Homo sapiens
1.4.1.3 ATP wild-type: activation at 1 mM, inhibition below and above, H454Y and S448P mutant enzymes: progressive increase in activity until 10 mM, inhibition above Homo sapiens
1.4.1.3 leucine wild-type, S448P, H454Y and R463A mutant enzymes Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.3 coexpression of wild-type and mutant enzymes with GroES and GroEl in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.3 H454Y lower basal activity but comparable maximal activity as wild-type Homo sapiens
1.4.1.3 R463A stimulatory effect of ADP is eliminated Homo sapiens
1.4.1.3 S448P unstable in Tris-buffer especially in the absence of allosteric activators, basal and maximal specific activity is lower than that from wild-type Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.3 ATP wild-type: inhibition at 0.1 mM and between 0.5-1.0 mM and above, activation at 1 mM, H454Y and S448P mutant enzyme: activation between 0.1-1 mM, inhibition above, R463A mutant enzyme: progressive inhibition between 0.01 and 10 mM Homo sapiens
1.4.1.3 diethylstilbestrol
-
 Homo sapiens
1.4.1.3 GTP little inhibition of H454Y mutant enzyme Homo sapiens
1.4.1.3 palmitoyl-CoA
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3 wild-type and mutant enzyme, Q-Sepharose, omega-aminopentyl column, GTP-agarose Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 19
-
 recombinant S448P mutant enzyme, basal activity Homo sapiens
1.4.1.3 55
-
 recombinant S448P mutant enzyme, maximal activity in the presence of 6 mM leucine Homo sapiens
1.4.1.3 69
-
 recombinant S448P mutant enzyme, maximal activity in the presence of 0.2 mM ADP Homo sapiens
1.4.1.3 73
-
 recombinant R463A mutant enzyme, basal activity Homo sapiens
1.4.1.3 74
-
 recombinant H454Y mutant enzyme, basal activity Homo sapiens
1.4.1.3 84
-
 recombinant wild-type enzyme, basal activity Homo sapiens
1.4.1.3 100
-
 recombinant R463A mutant enzyme, maximal activity in the presence of 6 mM leucine Homo sapiens
1.4.1.3 130
-
 recombinant wild-type enzyme, maximal activity in the presence of 0.2 mM ADP or 6 mM leucine Homo sapiens
1.4.1.3 130
-
 recombinant S448P mutant enzyme, maximal activity in the presence of 6 mM leucine Homo sapiens
1.4.1.3 140
-
 recombinant H454Y mutant enzyme, maximal activity in the presence of 0.2 mM ADP Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Homo sapiens 2-oxoglutarate + NH3 + NAD(P)H
-
 r

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.3 0.000013
-
 palmitoyl-CoA inhibition of recombinant S448P mutant enzyme Homo sapiens
1.4.1.3 0.000015
-
 palmitoyl-CoA inhibition of recombinant H454Y mutant enzyme Homo sapiens
1.4.1.3 0.00003
-
 palmitoyl-CoA inhibition of recombinant wild-type enzyme Homo sapiens
1.4.1.3 0.000042
-
 GTP inhibition of recombinant wild-type enzyme Homo sapiens
1.4.1.3 0.0002
-
 palmitoyl-CoA inhibition of recombinant R463A mutant enzyme Homo sapiens
1.4.1.3 0.00022
-
 GTP inhibition of recombinant R463A mutant enzyme Homo sapiens
1.4.1.3 0.00036
-
 diethylstilbestrol inhibition of recombinant H454Y mutant enzyme Homo sapiens
1.4.1.3 0.00074
-
 diethylstilbestrol inhibition of recombinant S448P mutant enzyme Homo sapiens
1.4.1.3 0.0017
-
 diethylstilbestrol inhibition of recombinant wild-type enzyme Homo sapiens
1.4.1.3 0.0031
-
 GTP inhibition of recombinant S448P mutant enzyme Homo sapiens
1.4.1.3 0.0051
-
 diethylstilbestrol inhibition of recombinant R463A mutant enzyme Homo sapiens
1.4.1.3 0.21
-
 GTP inhibition of recombinant H454Y mutant enzyme Homo sapiens