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Literature summary extracted from

  • Ahn, J.Y.; Lee, K.S.; Choi, S.Y.; Cho, S.W.
    Regulatory properties of glutamate dehydrogenase from Sulfolobus solfataricus (2000), Mol. Cells, 10, 25-31.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.3 additional information no inhibition or activation in the presence of ADP, GTP and leucine Saccharolobus solfataricus
1.4.1.3 o-phthalaldehyde 0.1 mM, 98% inhibition after 5 min at 60°C, competitive vs. 2-oxoglutarate and NADH Saccharolobus solfataricus
1.4.1.3 Phenylglyoxal 4 mM, 75% inhibition, uncompetitive vs. 2-oxoglutarate, noncompetitive vs. NADH Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 45000
-
 6 * 45000, SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 83
-
-
 Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
1.4.1.3 -20°C, 4°C, 25°C, 6 months, 5% loss of activity Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Saccharolobus solfataricus 2-oxoglutarate + NH3 + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer 6 * 45000, SDS-PAGE Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.3 0.03
-
 o-phthalaldehyde vs. 2-oxoglutarate Saccharolobus solfataricus
1.4.1.3 0.1
-
 o-phthalaldehyde vs. NADH Saccharolobus solfataricus
1.4.1.3 5
-
 Phenylglyoxal vs. 2-oxoglutarate Saccharolobus solfataricus
1.4.1.3 6
-
 Phenylglyoxal vs. NADH Saccharolobus solfataricus