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Literature summary extracted from

  • Maulik, P.; Ghosh, S.
    NADPH/NADH-dependent cold-labile glutamate dehydrogenase in Azospirillum brasilense. Purification and properties (1986), Eur. J. Biochem., 155, 595-602.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.3 glycerol, 20% v/v, protects against cold inactivation Azospirillum brasilense

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.3 alanine weak inhibition at pH 8.5, strong inhibition at pH 10.0 Azospirillum brasilense
1.4.1.3 glutamate weak inhibition at pH 8.5, strong inhibition at pH 10.0 Azospirillum brasilense
1.4.1.3 histidine weak inhibition at pH 8.5, strong inhibition at pH 10.0 Azospirillum brasilense
1.4.1.3 lysine weak inhibition at pH 8.5, strong inhibition at pH 10.0 Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.3 0.004
-
 NADP+ NADP+-dependent activity Azospirillum brasilense
1.4.1.3 0.013
-
 NADPH NADPH-dependent activity Azospirillum brasilense
1.4.1.3 0.25
-
 2-oxoglutarate NADPH-dependent activity Azospirillum brasilense
1.4.1.3 0.38
-
 NH3 biphasic Lineweaver-Burk plot suggests 2 Km values Azospirillum brasilense
1.4.1.3 0.5
-
 NADH NADH-dependent activity Azospirillum brasilense
1.4.1.3 5
-
 2-oxoglutarate NADH-dependent activity Azospirillum brasilense
1.4.1.3 10
-
 L-glutamate NADP+-dependent activity Azospirillum brasilense
1.4.1.3 66
-
 NH3 NADH-dependent activity Azospirillum brasilense
1.4.1.3 100
-
 NH3 biphasic Lineweaver-Burk plot suggests 2 Km values Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.3 48000
-
 6 * 48000, SDS-PAGE Azospirillum brasilense
1.4.1.3 285000
-
 gel filtration Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3
-
 Azospirillum brasilense

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 41.9
-
-
 Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 2-oxoglutarate + NH3 + NADH + H+
-
 Azospirillum brasilense L-glutamate + H2O + NAD+
-
 r
1.4.1.3 2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Azospirillum brasilense L-glutamate + H2O + NAD+
-
 r
1.4.1.3 2-oxoglutarate + NH3 + NADPH + H+ NH4Cl used in enzyme assay Azospirillum brasilense L-glutamate + H2O + NADP+
-
 r
1.4.1.3 L-glutamate + H2O + NAD(P)+ highly specific for 2-oxoglutarate and glutamate Azospirillum brasilense 2-oxoglutarate + NH3 + NAD(P)H
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.3 hexamer 6 * 48000, SDS-PAGE Azospirillum brasilense

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.3
-
-
 complete loss of activity after 5 h, 20% glycerol protects from inactivation Azospirillum brasilense
1.4.1.3 5
-
 moderately stable above Azospirillum brasilense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.3 3.33
-
 L-glutamate cofactor NAD+, value below 200.0 Azospirillum brasilense
1.4.1.3 61
-
 glutamate cofactor NADP+ Azospirillum brasilense
1.4.1.3 183
-
 2-oxoglutarate cofactor NADH Azospirillum brasilense
1.4.1.3 200
-
 NADPH NADPH-dependent activity Azospirillum brasilense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.3 8.4 10 NADPH + 2-oxoglutarate + NH3, potassium phosphate buffer Azospirillum brasilense
1.4.1.3 8.5 8.6 NADPH + NH3 + 2-oxoglutarate, Tris-HCl buffer Azospirillum brasilense

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.3 7 9 room temperature, 20% v/v glycerol, stable, room temperature, 1% v/v glycerol, 25% loss of activity after 48 h Azospirillum brasilense

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.3 1.1
-
 glutamate at pH 10.0 Azospirillum brasilense
1.4.1.3 3.7
-
 lysine at pH 10.0 Azospirillum brasilense
1.4.1.3 4.1
-
 glutamate at pH 8.5 Azospirillum brasilense
1.4.1.3 6.1
-
 histidine at pH 10.0 Azospirillum brasilense
1.4.1.3 6.2
-
 alanine at pH 10.0 Azospirillum brasilense
1.4.1.3 9.3
-
 lysine at pH 8.5 Azospirillum brasilense
1.4.1.3 23.2
-
 alanine at pH 8.5 Azospirillum brasilense