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Literature summary extracted from

  • Graham, L.D.; Griffin, T.O.; Beatty, R.E.; McCarthy, A.D.; Tipton, K.F.
    Purification of liver glutamate dehydrogenase by affinity precipitation and studies on its denaturation (1985), Biochim. Biophys. Acta, 828, 266-269.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.3 Bos taurus
-
-
-
1.4.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.3
-
 Rattus norvegicus
1.4.1.3
-
 Bos taurus
1.4.1.3 ammonium sulfate, DEAE-cellulose, affinity precipitation with adipo-N2,N2-dihydrazido-bis(N6-carboxymethyl-NAD+) Bos taurus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.4.1.3 complete loss of activity after incubation with 6 M guanidine hydrochloride or 7 M urea, no renaturation after dilution into 200 mM phosphate buffer Rattus norvegicus
1.4.1.3 complete loss of activity after incubation with 6 M guanidine hydrochloride or 7 M urea, no renaturation after dilution into 200 mM phosphate buffer Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.3 liver
-
 Rattus norvegicus
-
1.4.1.3 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.3 37
-
-
 Rattus norvegicus
1.4.1.3 40
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Rattus norvegicus 2-oxoglutarate + NH3 + NAD(P)H
-
 r
1.4.1.3 L-glutamate + H2O + NAD(P)+
-
 Bos taurus 2-oxoglutarate + NH3 + NAD(P)H
-
 r