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Literature summary extracted from

  • Lebbink, J.H.; Knapp, S.; van der Oost, J.; Rice, D.; Ladenstein, R.; de Vos, W.M.
    Engineering activity and stability of Thermotoga maritima glutamate dehydrogenase. II: construction of a 16-residue ion-pair network at the subunit interface (1999), J. Mol. Biol., 289, 357-369.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.2
-
 Thermotoga maritima
1.4.1.4 quadruple mutant S128R/T158E/N117R/S160E, homohexamer, 2.9 A resolution Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.2 G376K faster thermal inactivation, higher specific activity at 58°C Thermotoga maritima
1.4.1.2 N97D faster thermal inactivation Thermotoga maritima
1.4.1.2 N97D/G376K faster thermal inactivation, higher specific activity at 58°C Thermotoga maritima
1.4.1.4 N117R 80% activity of wild-type at optimum temperature for catalysis Thermotoga maritima
1.4.1.4 S128R same activity as wild-type at optimum temperature for catalysis Thermotoga maritima
1.4.1.4 S128R/T158E 120% activity of wild-type at optimum temperature for catalysis Thermotoga maritima
1.4.1.4 S128R/T158E/N117R same activity as wild-type at optimum temperature for catalysis Thermotoga maritima
1.4.1.4 S128R/T158E/N117R/S160E same activity as wild-type at optimum temperature for catalysis Thermotoga maritima
1.4.1.4 S128R/T158E/S160E same activity as wild-type at optimum temperature for catalysis Thermotoga maritima
1.4.1.4 T158E 60% activity of wild-type at optimum temperature for catalysis Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Thermotoga maritima
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Thermotoga maritima
-
-
-
1.4.1.4 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2 overexpression of mutants in Escherichia coli Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 2.5
-
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Thermotoga maritima 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homohexamer alpha6, 6 * ?, crystallization Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.4 55
-
 T158E-mutant Thermotoga maritima
1.4.1.4 58
-
 S128R-mutant Thermotoga maritima
1.4.1.4 60
-
 S128R/T158E/S160E-mutant Thermotoga maritima
1.4.1.4 63
-
 N117R-mutant and S128R/T158E/N117R/S160E-mutant Thermotoga maritima
1.4.1.4 65
-
 wild-type and S128R/T158E-mutant Thermotoga maritima
1.4.1.4 68
-
 S128R/T158E/N117R-mutant Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 85
-
 wild-type 50% activity after 209 min Thermotoga maritima
1.4.1.4 85
-
 quadruple mutant 50% activity after 5 min Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Thermotoga maritima
1.4.1.4 NADPH
-
 Thermotoga maritima