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Literature summary extracted from

  • Scheid, H.W.; Ehmke, A.; Hartmann, T.
    Plant NAD-dependent glutamate dehydrogenase. Purification, molecular properties and metal ion activation of the enzymes from Lemna minor and Pisum sativum (1980), Z. Naturforsch. C, 35, 213-221.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.2 Ca2+ required Pisum sativum
1.4.1.2 Ca2+ required Lemna minor
1.4.1.2 Zn2+ can replace Ca2+, requirement Pisum sativum
1.4.1.2 Zn2+ can replace Ca2+, requirement Lemna minor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 58500
-
 4 * 58500, SDS-PAGE Pisum sativum
1.4.1.2 58500
-
 4 * 58500, SDS-PAGE Lemna minor
1.4.1.2 230000
-
 gel filtration Lemna minor
1.4.1.2 230000
-
 sedimentation equilibrium analysis Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Lemna minor
-
-
-
1.4.1.2 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2
-
 Pisum sativum
1.4.1.2
-
 Lemna minor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.2 seed
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.2 401
-
-
 Pisum sativum
1.4.1.2 540
-
-
 Lemna minor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Pisum sativum 2-oxoglutarate + NH3 + NADH + H+
-
 ?
1.4.1.2 L-glutamate + H2O + NAD+
-
 Lemna minor 2-oxoglutarate + NH3 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.2 tetramer 4 * 58500, SDS-PAGE Pisum sativum
1.4.1.2 tetramer 4 * 58500, SDS-PAGE Lemna minor

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+
-
 Pisum sativum
1.4.1.2 NAD+
-
 Lemna minor
1.4.1.2 NADH
-
 Pisum sativum
1.4.1.2 NADH
-
 Lemna minor
1.4.1.2 NADP+ less than 1% of the activity with NAD+ Lemna minor
1.4.1.2 NADPH
-
 Pisum sativum
1.4.1.2 NADPH
-
 Lemna minor