BRENDA - Enzyme Database

Purification and properties of glutamate dehydrogenase from Vigna unguiculata (L.) Walp.

Fawole, M.O.; Boulter, D.; Planta 134, 97-102 (1977)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.4.1.2
repeated freezing and thawing: loss of activity
Vigna unguiculata
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.2
DL-valine
-
Vigna unguiculata
1.4.1.2
EDTA
-
Vigna unguiculata
1.4.1.2
isocitrate
-
Vigna unguiculata
1.4.1.2
L-aspartate
-
Vigna unguiculata
1.4.1.2
p-hydroxymercuribenzoate
-
Vigna unguiculata
1.4.1.2
Pyruvic acid
-
Vigna unguiculata
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.1.2
Vigna unguiculata
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.1.2
-
Vigna unguiculata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.1.2
seedling
-
Vigna unguiculata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.1.2
additional information
-
-
Vigna unguiculata
Storage Stability
EC Number
Storage Stability
Organism
1.4.1.2
-20°C, several months
Vigna unguiculata
1.4.1.2
-5°C, 20% loss of activity after 3 months
Vigna unguiculata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.2
L-glutamate + H2O + NAD+
-
391553
Vigna unguiculata
2-oxoglutarate + NH3 + NADH + H+
-
-
-
?
1.4.1.2
L-norvaline + H2O + NAD+
at 40% the rate
391553
Vigna unguiculata
2-oxopentanoate + NH3 + NADH
-
-
-
?
1.4.1.2
L-serine + H2O + NAD+
deamination at 29% the rate of L-glutamate deamination
391553
Vigna unguiculata
3-hydroxy-2-oxopropanoate + NH3 + NADH
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.2
NAD+
-
Vigna unguiculata
1.4.1.2
NADH
-
Vigna unguiculata
1.4.1.2
NADPH
activity with NADH, NADPH and NAD+ in the ratio 126:2:1
Vigna unguiculata
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.1.2
NAD+
-
Vigna unguiculata
1.4.1.2
NADH
-
Vigna unguiculata
1.4.1.2
NADPH
activity with NADH, NADPH and NAD+ in the ratio 126:2:1
Vigna unguiculata
General Stability (protein specific)
EC Number
General Stability
Organism
1.4.1.2
repeated freezing and thawing: loss of activity
Vigna unguiculata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.1.2
DL-valine
-
Vigna unguiculata
1.4.1.2
EDTA
-
Vigna unguiculata
1.4.1.2
isocitrate
-
Vigna unguiculata
1.4.1.2
L-aspartate
-
Vigna unguiculata
1.4.1.2
p-hydroxymercuribenzoate
-
Vigna unguiculata
1.4.1.2
Pyruvic acid
-
Vigna unguiculata
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.1.2
-
Vigna unguiculata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.4.1.2
seedling
-
Vigna unguiculata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.1.2
additional information
-
-
Vigna unguiculata
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.4.1.2
-20°C, several months
Vigna unguiculata
1.4.1.2
-5°C, 20% loss of activity after 3 months
Vigna unguiculata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.1.2
L-glutamate + H2O + NAD+
-
391553
Vigna unguiculata
2-oxoglutarate + NH3 + NADH + H+
-
-
-
?
1.4.1.2
L-norvaline + H2O + NAD+
at 40% the rate
391553
Vigna unguiculata
2-oxopentanoate + NH3 + NADH
-
-
-
?
1.4.1.2
L-serine + H2O + NAD+
deamination at 29% the rate of L-glutamate deamination
391553
Vigna unguiculata
3-hydroxy-2-oxopropanoate + NH3 + NADH
-
-
-
?