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Literature summary extracted from

  • Misono, H.; Ogasawara, M.; Nagasaki, S.
    Characterization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum and its distribution in bacteria (1986), Agric. Biol. Chem., 50, 2729-2734.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.16 Cu2+
-
 Brevibacterium sp.
1.4.1.16 Cu2+ 0.01 mM, 82% inhibition Corynebacterium glutamicum
1.4.1.16 D-2,6-diaminopimelate 10 mM, 49% inhibition Corynebacterium glutamicum
1.4.1.16 D-2,6-diaminopimelate
-
 Lysinibacillus sphaericus
1.4.1.16 HgCl2
-
 Brevibacterium sp.
1.4.1.16 HgCl2 0.01 mM, 92% inhibition Corynebacterium glutamicum
1.4.1.16 HgCl2
-
 Lysinibacillus sphaericus
1.4.1.16 iodoacetate
-
 Brevibacterium sp.
1.4.1.16 iodoacetate 1 mM, 77% inhibition Corynebacterium glutamicum
1.4.1.16 iodoacetate
-
 Lysinibacillus sphaericus
1.4.1.16 L-2,6-diaminopimelate 10 mM, 27% inhibition Corynebacterium glutamicum
1.4.1.16 L-2,6-diaminopimelate
-
 Lysinibacillus sphaericus
1.4.1.16 L-cysteine 5 mM, 99% inhibition Corynebacterium glutamicum
1.4.1.16 Li+ 1 mM, 32% inhibition Corynebacterium glutamicum
1.4.1.16 N-ethylmaleimide
-
 Brevibacterium sp.
1.4.1.16 N-ethylmaleimide 1 mM, 39% inhibition Corynebacterium glutamicum
1.4.1.16 N-ethylmaleimide
-
 Lysinibacillus sphaericus
1.4.1.16 Ni2+
-
 Brevibacterium sp.
1.4.1.16 Ni2+ 1 mM, 36% inhibition Corynebacterium glutamicum
1.4.1.16 p-chloromercuribenzoate
-
 Corynebacterium glutamicum
1.4.1.16 p-chloromercuribenzoate
-
 Lysinibacillus sphaericus
1.4.1.16 thioglycolate 5 mM, 62% inhibition Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.16 0.083 0.14 NADP+
-
 Corynebacterium glutamicum
1.4.1.16 0.083 0.14 NADP+
-
 Lysinibacillus sphaericus
1.4.1.16 0.083 0.14 NADP+
-
 Brevibacterium sp.
1.4.1.16 0.13
-
 NADPH
-
 Corynebacterium glutamicum
1.4.1.16 0.28
-
 L-2-amino-6-oxopimelate
-
 Corynebacterium glutamicum
1.4.1.16 2 10 NAD+
-
 Corynebacterium glutamicum
1.4.1.16 2 10 NAD+
-
 Brevibacterium sp.
1.4.1.16 10
-
 NAD+
-
 Corynebacterium glutamicum
1.4.1.16 36
-
 NH3
-
 Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.16 39000
-
 2 * 39000, SDS-PAGE Corynebacterium glutamicum
1.4.1.16 70000
-
-
 Brevibacterium sp.
1.4.1.16 70000
-
 gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Lysinibacillus sphaericus
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Corynebacterium glutamicum
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Pseudomonas fragi
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Brevibacterium sp.
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Morganella morganii
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Providencia rettgeri
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Pseudomonas sp. 'cruciviae'
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH Glutamicibacter protophormiae
-
 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Achromobacter polymorph
-
 very low activity
-
1.4.1.16 Achromobacter superficialis
-
 very low activity
-
1.4.1.16 Agrobacterium tumefaciens
-
 very low activity
-
1.4.1.16 Alcaligenes viscolactis
-
 low activity
-
1.4.1.16 Bacillus amyloliquefaciens
-
 very low activity
-
1.4.1.16 Bacillus cereus
-
 very low activity
-
1.4.1.16 Bacillus licheniformis
-
 very low activity
-
1.4.1.16 Bacterium mycoides
-
 very low activity
-
1.4.1.16 Brevibacillus brevis
-
 low activity
-
1.4.1.16 Brevibacterium sp.
-
-
-
1.4.1.16 Corynebacterium ammoniagenes
-
 low activity
-
1.4.1.16 Corynebacterium glutamicum
-
 strain ATCC 13032
-
1.4.1.16 Corynebacterium pseudodiphtheriticum
-
 low activity
-
1.4.1.16 Enterobacter cloacae
-
 low activity
-
1.4.1.16 Flavobacterium suaveolens
-
 low activity
-
1.4.1.16 Glutamicibacter protophormiae
-
-
-
1.4.1.16 Hafnia alvei
-
 very low activity
-
1.4.1.16 Kocuria rosea
-
 very low activity
-
1.4.1.16 Lysinibacillus sphaericus
-
-
-
1.4.1.16 Micrococcus luteus
-
 low activity
-
1.4.1.16 Morganella morganii
-
-
-
1.4.1.16 no activity in Escherichia coli
-
 strains ICR 0010, K12, ICR 0050 and IFO 3301
-
1.4.1.16 no activity in Klebsiella pneumoniae
-
 strains IFO 125059 and 12019
-
1.4.1.16 no activity in Staphylococcus aureus
-
-
-
1.4.1.16 Providencia rettgeri
-
-
-
1.4.1.16 Pseudomonas aeruginosa
-
 very low activity
-
1.4.1.16 Pseudomonas alkanolytica
-
 very low activity
-
1.4.1.16 Pseudomonas chlororaphis subsp. aureofaciens
-
 very low activity
-
1.4.1.16 Pseudomonas fragi
-
-
-
1.4.1.16 Pseudomonas putida
-
 very low activity
-
1.4.1.16 Pseudomonas sp. 'cruciviae'
-
-
-
1.4.1.16 Terrabacter tumescens
-
 very low activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.16
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.16 131.6
-
-
 Corynebacterium glutamicum

Storage Stability

EC Number Storage Stability Organism
1.4.1.16 -20°C, 0.01 M potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, 1 year Corynebacterium glutamicum
1.4.1.16 -20°C, 0.01 M potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 10% glycerol, 1 year Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Brevibacillus brevis meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Lysinibacillus sphaericus meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Kocuria rosea meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas aeruginosa meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas putida meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Bacillus cereus meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Corynebacterium glutamicum meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Micrococcus luteus meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Bacillus licheniformis meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Bacillus amyloliquefaciens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas fragi meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Enterobacter cloacae meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Agrobacterium tumefaciens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Brevibacterium sp. meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Morganella morganii meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Corynebacterium ammoniagenes meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Hafnia alvei meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Providencia rettgeri meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas sp. 'cruciviae' meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas chlororaphis subsp. aureofaciens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Corynebacterium pseudodiphtheriticum meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Alcaligenes viscolactis meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Achromobacter polymorph meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Pseudomonas alkanolytica meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Flavobacterium suaveolens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Glutamicibacter protophormiae meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Terrabacter tumescens meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Bacterium mycoides meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NAD(P)H
-
 Achromobacter superficialis meso-2,6-diaminoheptanedioate + H2O + NAD(P)+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Lysinibacillus sphaericus meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Corynebacterium glutamicum meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Pseudomonas fragi meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Brevibacterium sp. meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Morganella morganii meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Providencia rettgeri meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Pseudomonas sp. 'cruciviae' meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 L-2-amino-6-oxoheptanedioate + NH3 + NADPH
-
 Glutamicibacter protophormiae meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 r
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Corynebacterium glutamicum L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 r
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Brevibacterium sp. L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.16 dimer
-
 Lysinibacillus sphaericus
1.4.1.16 dimer
-
 Brevibacterium sp.
1.4.1.16 dimer 2 * 39000, SDS-PAGE Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.16 45
-
 unstable above Corynebacterium glutamicum
1.4.1.16 45
-
 unstable above Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.16 7.9
-
 reductive amination of 2-amino-6-oxopimelate Corynebacterium glutamicum
1.4.1.16 9
-
 oxidative deamination of meso-2,6-diaminopimelate Corynebacterium glutamicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.16 6.5 7
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.16 NADH 3% activity of that with NADP+ Corynebacterium glutamicum
1.4.1.16 NADH 3% activity of that with NADP+ Brevibacterium sp.
1.4.1.16 NADPH
-
 Corynebacterium glutamicum
1.4.1.16 NADPH
-
 Lysinibacillus sphaericus
1.4.1.16 NADPH
-
 Brevibacterium sp.
1.4.1.16 NADPH
-
 Glutamicibacter protophormiae
1.4.1.16 NADPH
-
 Morganella morganii
1.4.1.16 NADPH
-
 Providencia rettgeri
1.4.1.16 NADPH
-
 Pseudomonas sp. 'cruciviae'
1.4.1.16 NADPH
-
 Pseudomonas fragi
1.4.1.16 NADPH
-
 Flavobacterium suaveolens
1.4.1.16 NADPH
-
 Micrococcus luteus
1.4.1.16 NADPH
-
 Alcaligenes viscolactis
1.4.1.16 NADPH
-
 Corynebacterium pseudodiphtheriticum
1.4.1.16 NADPH
-
 Enterobacter cloacae
1.4.1.16 NADPH
-
 Corynebacterium ammoniagenes
1.4.1.16 NADPH
-
 Brevibacillus brevis
1.4.1.16 NADPH
-
 Hafnia alvei
1.4.1.16 NADPH
-
 Terrabacter tumescens
1.4.1.16 NADPH
-
 Agrobacterium tumefaciens
1.4.1.16 NADPH
-
 Pseudomonas putida
1.4.1.16 NADPH
-
 Bacillus cereus
1.4.1.16 NADPH
-
 Bacillus amyloliquefaciens
1.4.1.16 NADPH
-
 Bacillus licheniformis
1.4.1.16 NADPH
-
 Bacterium mycoides
1.4.1.16 NADPH
-
 Achromobacter superficialis
1.4.1.16 NADPH
-
 Achromobacter polymorph
1.4.1.16 NADPH
-
 Kocuria rosea
1.4.1.16 NADPH
-
 Pseudomonas chlororaphis subsp. aureofaciens
1.4.1.16 NADPH
-
 Pseudomonas alkanolytica
1.4.1.16 NADPH
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.16 2
-
 D-2,6-diaminopimelate
-
 Corynebacterium glutamicum
1.4.1.16 8.5
-
 L-2,6-diaminopimelate
-
 Corynebacterium glutamicum