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Literature summary extracted from

  • Miyatake, K.; Kitaoka, S.
    NADH-dependent glutamate synthase in Euglena gracilis z (1981), Agric. Biol. Chem., 45, 1727-1729.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.14 additional information not activated by pyridoxal 5’-phosphate and pyridoxamine 5’-phosphate Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.14 azaserine completely inhibited by 1 mM, glutamine analog Euglena gracilis
1.4.1.14 additional information not inhibited by vitamin B6-enzyme inhibitors Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.14 0.015
-
 2-oxoglutarate
-
 Euglena gracilis
1.4.1.14 0.02
-
 NADH
-
 Euglena gracilis
1.4.1.14 0.3
-
 L-glutamine
-
 Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.1.14 cytosol
-
 Euglena gracilis 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.14 Euglena gracilis
-
 Z
-
1.4.1.14 Euglena gracilis Z
-
 Z
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH absolute specificity for 2-oxoglutarate Euglena gracilis L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Euglena gracilis L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Euglena gracilis L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH absolute specificity for 2-oxoglutarate Euglena gracilis Z L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Euglena gracilis Z L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Euglena gracilis Z L-glutamate + NAD+
-
 ?
1.4.1.14 additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Euglena gracilis ?
-
 ?
1.4.1.14 additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Euglena gracilis Z ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.14 30
-
-
 Euglena gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.14 45
-
 rapid inactivation above Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.14 7.5
-
-
 Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.14 additional information no activity with NADPH Euglena gracilis
1.4.1.14 NADH specific for NADH as reductant Euglena gracilis