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Literature summary extracted from

  • Singhal, R.K.; Krishnan, I.S.; Dua, R.D.
    Stabilization, purification, and characterization of glutamate synthase from Clostridium pasteurianum (1989), Biochemistry, 28, 7928-7935.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.1.14 2-oxoglutarate, 2-mercaptoethanol and EDTA stabilize and have a synergystic effect Clostridium pasteurianum
1.4.1.14 sodium dithionite and glutamine stabilize Clostridium pasteurianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.14 asparagine slight inhibition Clostridium pasteurianum
1.4.1.14 Co2+ slight inhibition Clostridium pasteurianum
1.4.1.14 Cu2+ Cu2+ and Fe2+ are most inhibitory metal ions Clostridium pasteurianum
1.4.1.14 Fe2+ Cu2+ and Fe2+ are most inhibitory metal ions Clostridium pasteurianum
1.4.1.14 glycine slight inhibition Clostridium pasteurianum
1.4.1.14 L-glutamate 4 mM, 20% inhibition, product inhibition Clostridium pasteurianum
1.4.1.14 L-histidine 25 mM, 20-30% inhibition Clostridium pasteurianum
1.4.1.14 methionine 25 mM L-methionine, 20-30% inhibition Clostridium pasteurianum
1.4.1.14 Mg2+ slight inhibition Clostridium pasteurianum
1.4.1.14 additional information not inhibited by Mn2+ and Ca2+ Clostridium pasteurianum
1.4.1.14 pyruvate slight inhibition, analogue of 2-oxoglutarate Clostridium pasteurianum
1.4.1.14 serine 25 mM L-serine, 20-30% inhibition Clostridium pasteurianum
1.4.1.14 Sodium dithionite strong inhibitory effect Clostridium pasteurianum
1.4.1.14 tryptophan 25 mM L-tryptophan, 20-30% inhibition Clostridium pasteurianum
1.4.1.14 Zn2+ slight inhibition Clostridium pasteurianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.14 0.11
-
 NADH
-
 Clostridium pasteurianum
1.4.1.14 0.16
-
 L-glutamine
-
 Clostridium pasteurianum
1.4.1.14 0.17
-
 2-oxoglutarate
-
 Clostridium pasteurianum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.14 additional information absence of flavin and iron Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.14 17500
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
1.4.1.14 31000
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
1.4.1.14 68000
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
1.4.1.14 86000
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
1.4.1.14 91000
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
1.4.1.14 590000
-
 gel filtration Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Clostridium pasteurianum regulatory role in biosynthesis pathways involving glutamate, early acceptor of fixed nitrogen glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Clostridium pasteurianum ammonia assimilation glutamate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.14 Clostridium pasteurianum
-
 strain ATCC 6013
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.14 58fold purification Clostridium pasteurianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.14 3.8
-
-
 Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH 1 mol 2-oxoglutarate and 1 mol glutamine are consumed per mol NADH oxidized Clostridium pasteurianum L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Clostridium pasteurianum L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Clostridium pasteurianum L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH regulatory role in biosynthesis pathways involving glutamate, early acceptor of fixed nitrogen Clostridium pasteurianum glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH ammonia assimilation Clostridium pasteurianum glutamate + NAD+
-
 ?
1.4.1.14 additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Clostridium pasteurianum ?
-
 ?
1.4.1.14 additional information no activity with albizziin, 6-diazo-5-oxo-L-norvaline, azaserine, methionine sulfoximine, FADH or FMNH Clostridium pasteurianum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.14 decamer 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.14 30
-
 assay at Clostridium pasteurianum
1.4.1.14 50
-
-
 Clostridium pasteurianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.14 35
-
 enzyme loses activity after incubation for 10 min Clostridium pasteurianum
1.4.1.14 50
-
 half-life: 10 min, presence of 2-oxoglutarate and L-glutamine stabilizes against thermal denaturation, whereas preincubation with NADH or L-glutamate causes more heat sensitivity Clostridium pasteurianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.14 7.2
-
 broad pH profile with optimum at pH 7.2 Clostridium pasteurianum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.14 additional information no activity with NADPH Clostridium pasteurianum
1.4.1.14 additional information absence of flavin and iron Clostridium pasteurianum
1.4.1.14 NADH specific for NADH as reductant Clostridium pasteurianum