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Literature summary extracted from

  • Chen, F.L.; Cullimore, J.V.
    Two isoenzymes of NADH-dependent glutamate synthase in root nodules of Phaseolus vulgaris L.. Purification, properties and activity changes during nodule development (1988), Plant Physiol., 88, 1411-1417.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.4.1.14 2-mercaptoethanol stabilizes Phaseolus vulgaris
1.4.1.14 dithiothreitol stabilizes Phaseolus vulgaris
1.4.1.14 EDTA stabilizes Phaseolus vulgaris
1.4.1.14 highly labile enzyme activity Phaseolus vulgaris
1.4.1.14 phenylmethylsulphonyl fluoride is used as precaution against proteolysis Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.14 azaserine completely inhibited by 0.4 mM Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.14 0.0052
-
 NADH NADH-GOGAT II Phaseolus vulgaris
1.4.1.14 0.014
-
 NADH NADH-GOGAT I Phaseolus vulgaris
1.4.1.14 0.022
-
 2-oxoglutarate NADH-GOGAT I Phaseolus vulgaris
1.4.1.14 0.087
-
 2-oxoglutarate NADH-GOGAT II Phaseolus vulgaris
1.4.1.14 0.24
-
 L-glutamine NADH-GOGAT II Phaseolus vulgaris
1.4.1.14 0.77
-
 L-glutamine NADH-GOGAT I Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.14 200000
-
 NADH-GOGAT II, gel filtration Phaseolus vulgaris
1.4.1.14 240000
-
 NADH-GOGAT I, gel filtration Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH Phaseolus vulgaris ammonia assimilation glutamate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.14 Phaseolus vulgaris
-
 2 isoenzymes: NADH-GOGAT I and NADH-GOGAT II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.14 2 isoenzymes: NADH-GOGAT I with 877fold purification and NADH-GOGAT II with 631fold purification Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.14 root nodule NADH-GOGAT II is twice as active as NADH-GOGAT I in mature nodules Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.14 3.8
-
 NADH-GOGAT I Phaseolus vulgaris
1.4.1.14 8.05
-
 NADH-GOGAT II Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH specific for NADH Phaseolus vulgaris L-glutamate + NAD+
-
 ?
1.4.1.14 L-glutamine + 2-oxoglutarate + NADH ammonia assimilation Phaseolus vulgaris glutamate + NAD+
-
 ?
1.4.1.14 additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Phaseolus vulgaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.14 monomer 1 * 200000, SDS-PAGE Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.14 30
-
 NADH-GOGAT I/II Phaseolus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.14 additional information
-
 very sensitive to low temperatures Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.14 8
-
 NADH-GOGAT I, broad pH-optimum centering at pH 8 Phaseolus vulgaris
1.4.1.14 8.5
-
 NADH-GOGAT II, narrow pH-optimum of 8.5 Phaseolus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.14 NADH specific for NADH as reductant Phaseolus vulgaris