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Literature summary extracted from

  • Rachim, M.A.; Nicholas, D.J.D.
    Glutamine synthetase and glutamate synthase from Sclerotinia Schlerotiorum (1985), Phytochemistry, 24, 2541-2548.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 2,2'-bipyridyl 88% inhibition at 10 mM Sclerotinia sclerotiorum
1.4.1.13 ADP
-
 Sclerotinia sclerotiorum
1.4.1.13 AMP
-
 Sclerotinia sclerotiorum
1.4.1.13 arginine less than 45% inhibition Sclerotinia sclerotiorum
1.4.1.13 ATP restricts activity by 47% at 5 mM Sclerotinia sclerotiorum
1.4.1.13 azaserine 68% inhibition at 1 mM Sclerotinia sclerotiorum
1.4.1.13 citrate slight Sclerotinia sclerotiorum
1.4.1.13 D-glutamate
-
 Sclerotinia sclerotiorum
1.4.1.13 DL-methionine DL-sulfoximine
-
 Sclerotinia sclerotiorum
1.4.1.13 L-leucine less than 45% inhibition Sclerotinia sclerotiorum
1.4.1.13 L-malate 40% inhibition at 5 mM Sclerotinia sclerotiorum
1.4.1.13 o-phenanthroline complete inhibition at 10 mM Sclerotinia sclerotiorum
1.4.1.13 oxalate 48% inhibition at 5 mM Sclerotinia sclerotiorum
1.4.1.13 oxaloacetate
-
 Sclerotinia sclerotiorum
1.4.1.13 p-chloromercuribenzoate complete inhibition at 0.1 mM Sclerotinia sclerotiorum
1.4.1.13 phenylalanine 78% inhibition at 10 mM Sclerotinia sclerotiorum
1.4.1.13 pyruvate
-
 Sclerotinia sclerotiorum
1.4.1.13 sodium arsenite 57% inhibition at 10 mM Sclerotinia sclerotiorum
1.4.1.13 succinate
-
 Sclerotinia sclerotiorum
1.4.1.13 valine less than 45% inhibition Sclerotinia sclerotiorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.035
-
 NADPH
-
 Sclerotinia sclerotiorum
1.4.1.13 0.35
-
 alpha-ketoglutarate
-
 Sclerotinia sclerotiorum
1.4.1.13 2.6
-
 L-glutamine
-
 Sclerotinia sclerotiorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH Sclerotinia sclerotiorum main route for assimilation of ammonium compounds L-glutamate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Sclerotinia sclerotiorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Blue-Sepharose Sclerotinia sclerotiorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 7.6
-
 partially purified enzyme Sclerotinia sclerotiorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH main route for assimilation of ammonium compounds Sclerotinia sclerotiorum L-glutamate + NADP+
-
 ?
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Sclerotinia sclerotiorum L-glutamate + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 NADH no activity with NADH Sclerotinia sclerotiorum
1.4.1.13 NADPH
-
 Sclerotinia sclerotiorum