EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | DL-methionine DL-sulfoximine | - |
Bacillus subtilis | |
1.4.1.13 | fumarate | potent inhibitor of the glutamine-dependent activity | Bacillus subtilis | |
1.4.1.13 | L-glutamate | noncompetitive inhibitor with respect to both 2-oxoglutarate and L-glutamine | Bacillus subtilis | |
1.4.1.13 | L-malate | potent inhibitor of the glutamine-dependent activity | Bacillus subtilis | |
1.4.1.13 | L-methionine DL-sulfoximine | inhibits the glutamine-dependent reaction | Bacillus subtilis | |
1.4.1.13 | NAD+ | competitive inhibition with NADH, noncompetitive inhibition with 2-oxoglutarate or L-glutamine | Bacillus subtilis | |
1.4.1.13 | NADP+ | competitive inhibition with NADPH, noncompetitive inhibition with 2-oxoglutarate or L-glutamine | Bacillus subtilis | |
1.4.1.13 | p-chloromercuribenzoate | inhibits the glutamine-dependent reaction | Bacillus subtilis | |
1.4.1.13 | pyridoxal-5'-phosphate | 52% inhibition of the glutamine-dependent reaction at 5 mM | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.13 | 0.006 | - |
NADPH | in the presence of a fixed concentration of L-glutamine, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
1.4.1.13 | 0.007 | - |
NADPH | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of L-glutamine | Bacillus subtilis | |
1.4.1.13 | 0.007 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of NADPH, and varied concentrations of L-glutamine | Bacillus subtilis | |
1.4.1.13 | 0.01 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of NADH, and varied concentrations of L-glutamine | Bacillus subtilis | |
1.4.1.13 | 0.012 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of L-glutamine, and varied concentrations of NADH | Bacillus subtilis | |
1.4.1.13 | 0.013 | - |
alpha-ketoglutarate | in the presence of a fixed concentration of L-glutamine, and varied concentrations of NADPH | Bacillus subtilis | |
1.4.1.13 | 0.091 | - |
NADH | in the presence of a fixed concentration of L-glutamine, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
1.4.1.13 | 0.102 | - |
L-glutamine | in the presence of a fixed concentration of NADPH, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
1.4.1.13 | 0.108 | - |
L-glutamine | in the presence of a fixed concentration of NADH, and varied concentrations of alpha-ketoglutarate | Bacillus subtilis | |
1.4.1.13 | 0.113 | - |
NADH | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of L-glutamine | Bacillus subtilis | |
1.4.1.13 | 0.115 | - |
L-glutamine | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of NADPH | Bacillus subtilis | |
1.4.1.13 | 0.12 | - |
L-glutamine | in the presence of a fixed concentration of alpha-ketoglutarate, and varied concentrations of NADH | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | Iron | iron-sulfur protein | Bacillus subtilis | |
1.4.1.13 | Iron | 7.4 g-atoms of non-heme iron per mol of enzyme | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 56000 | - |
1 * 160000 + 1 * 56000 | Bacillus subtilis |
1.4.1.13 | 160000 | - |
1 * 160000 + 1 * 56000 | Bacillus subtilis |
1.4.1.13 | 210000 | - |
sucrose density sedimentation | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.13 | Bacillus subtilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.13 | - |
Bacillus subtilis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.4.1.13 | 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+ | mechanism | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADH + H+ | - |
Bacillus subtilis | L-glutamate + NAD+ | - |
r | |
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | - |
Bacillus subtilis | L-glutamate + NADP+ | - |
? | |
1.4.1.13 | NH3 + 2-oxoglutarate + NADPH + H+ | 24% relative activity to L-glutamine with NADPH as electron donor and 6.3% relative activity to L-glutamine with NADH as electron donor | Bacillus subtilis | L-glutamate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.13 | dimer | 1 * 160000 + 1 * 56000 | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 6.9 | - |
NADH-glutamine dependent activity | Bacillus subtilis |
1.4.1.13 | 7.8 | - |
NADPH-glutamine dependent activity | Bacillus subtilis |
1.4.1.13 | 9.4 | - |
NADPH-NH3 dependent activity | Bacillus subtilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | flavin | 0.83 mol of FMN and 0.81 mol of FAD per mol of enzyme | Bacillus subtilis | |
1.4.1.13 | additional information | the enzyme contains 8.7 g atoms per mol | Bacillus subtilis | |
1.4.1.13 | NADH | 21% relative activity to NADPH | Bacillus subtilis | |
1.4.1.13 | NADPH | - |
Bacillus subtilis |