Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mäntsälä, P.; Zalkin, H.
    Properties of apoglutamate synthase and comparison with glutamate dehydrogenase (1976), J. Biol. Chem., 251, 3300-3305.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 3-Bromo-2-oxoglutarate
-
 Escherichia coli
1.4.1.13 4-Iodoacetamidosalicylate inactivation is faster at pH 4.6 to 5.5 compared to pH 6.5 to 7.2 Escherichia coli
1.4.1.13 Bromopyruvate
-
 Escherichia coli
1.4.1.13 iodoacetamide
-
 Escherichia coli
1.4.1.13 p-chloromercuribenzoate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.014
-
 NADPH NH3-dependent glutamate synthase and apoglutamate synthase Escherichia coli
1.4.1.13 0.24
-
 2-oxoglutarate NH3-dependent glutamate synthase and apoglutamate synthase Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 additional information
-
-
 Escherichia coli
1.4.1.13 0.24
-
 apoenzyme lacking flavin and non-heme iron, glutamine-dependent activity Escherichia coli
1.4.1.13 0.92
-
 native enzyme, NH3-dependent activity Escherichia coli
1.4.1.13 4.45
-
 apoenzyme lacking flavin and non-heme iron, NH3-dependent activity Escherichia coli
1.4.1.13 18.64
-
 native enzyme, glutamine-dependent activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ both native and apoglutamate synthase catalyze NADP+ reduction at approximately 12% the rate of NADPH oxidation Escherichia coli L-glutamate + NADP+
-
 r
1.4.1.13 additional information NH3-dependent activity is increased approximately 5fold in apoglutamate synthase lacking flavin and non-heme iron Escherichia coli ?
-
 ?
1.4.1.13 NH3 + 2-oxoglutarate + NADPH + H+
-
 Escherichia coli L-glutamate + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.13 8.4
-
 for glutamate synthesis, NH3-dependent glutamate synthase and apoenzyme Escherichia coli
1.4.1.13 9.4
-
 optimum for glutamate-dependent reduction of NADP+, NH3-dependent enzyme, apoglutamate synthase enzyme Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.13 0.86
-
 p-chloromercuribenzoate
-
 Escherichia coli