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Literature summary extracted from

  • Hemmilä, I.A.; Mäntsälä, P.I.
    Purification and properties of glutamate synthase and glutamate dehydrogenase from Bacillus megaterium (1978), Biochem. J., 173, 45-52.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.13 dimethyl suberimidate increases NH3-dependent activity Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 dimethyl suberimidate inactivates glutamine-dependent activity Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.4 0.0087
-
 NADPH reductive amination Priestia megaterium
1.4.1.4 0.05
-
 NADP+ oxidative deamination Priestia megaterium
1.4.1.4 0.36
-
 2-oxoglutarate reductive amination Priestia megaterium
1.4.1.4 22
-
 NH4+ reductive amination Priestia megaterium
1.4.1.4 29
-
 L-glutamate oxidative deamination Priestia megaterium
1.4.1.13 0.0071
-
 NADPH
-
 Priestia megaterium
1.4.1.13 0.009
-
 2-oxoglutarate
-
 Priestia megaterium
1.4.1.13 0.2
-
 L-glutamine
-
 Priestia megaterium
1.4.1.13 22
-
 NH4Cl
-
 Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 47000
-
 alpha6, 6 * 47000, SDS-PAGE Priestia megaterium
1.4.1.4 270000
-
 sucrose-density-gradient centrifugation Priestia megaterium
1.4.1.13 55000
-
 x * 142000 + x * 55000, SDS-PAGE Priestia megaterium
1.4.1.13 142000
-
 x * 142000 + x * 55000, SDS-PAGE Priestia megaterium
1.4.1.13 840000
-
 sucrose density-gradient centrifugation Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 L-glutamate + NADP+ + H2O Priestia megaterium
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Priestia megaterium
-
-
-
1.4.1.13 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using streptomycin sulfate treatment, ammonium sulfate precipitation, column chromatography on Ultrogel AcA 22 and DEAE-Sephadex A-50, ultrafiltration with an Amicon PM 30 membrane and chromatography on hydroxyapatite column Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 23.8
-
-
 Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoglutarate + NH4+ + NADPH
-
 Priestia megaterium L-glutamate + NADP+
-
 ?
1.4.1.4 L-glutamate + NADP+ + H2O
-
 Priestia megaterium 2-oxoglutarate + NADPH + NH3
-
 r
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ in the reverse reaction ammonia can act instead of glutamine, but more slowly Priestia megaterium L-glutamate + NADP+
-
 ?
1.4.1.13 NH3 + 2-oxoglutarate + NADPH + H+ 2% to 4% relative activity to L-glutamine Priestia megaterium L-glutamate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.4 homohexamer alpha6, 6 * 47000, SDS-PAGE Priestia megaterium
1.4.1.13 ? x * 142000 + x * 55000, SDS-PAGE Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 7.9
-
 reductive amination Priestia megaterium
1.4.1.4 9
-
 oxidative deamination Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Priestia megaterium
1.4.1.4 NADPH
-
 Priestia megaterium
1.4.1.13 NADPH
-
 Priestia megaterium