Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schmidt, C.N.G.; Jervis, L.
    Partial purification and characterization of glutamate synthase from a thermophilic bacillus (1982), J. Gen. Microbiol., 128, 1713-1718.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 azaserine in both the L-glutamine- and NH4Cl-dependent reactions Geobacillus stearothermophilus
1.4.1.13 Cibacron blue 3GA
-
 Geobacillus stearothermophilus
1.4.1.13 L-methionine sulfone
-
 Geobacillus stearothermophilus
1.4.1.13 NADPH rapid inactivation in absence of L-glutamine and 2-oxoglutarate, capable of causing 50% inactivation in 5 min at a concentration of 1 mM Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.015
-
 2-oxoglutarate
-
 Geobacillus stearothermophilus
1.4.1.13 0.022
-
 NADPH
-
 Geobacillus stearothermophilus
1.4.1.13 0.029
-
 L-glutamine
-
 Geobacillus stearothermophilus
1.4.1.13 44
-
 NH4Cl
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.13 160000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Geobacillus stearothermophilus
-
-
-
1.4.1.13 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using ammonium sulfate fractionation, gel filtration on Sephacryl S-300 and affinity chromatography on NADPH-Sepharose Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 additional information
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Geobacillus stearothermophilus 2-oxoglutarate + NH3 + NADH + H+
-
 ?
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ in the reverse reaction ammonia can act instead of glutamine, but more slowly Geobacillus stearothermophilus L-glutamate + NADP+
-
 ir
1.4.1.13 NH3 + 2-oxoglutarate + NADPH + H+ the rate is only 10% to 15% that of the L-glutamine-dependent reaction Geobacillus stearothermophilus L-glutamate + NADP+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.13 75
-
 glutamine-dependent reaction Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.13 80
-
 10 min, 35% loss of activity Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.13 8
-
 glutamine-dependent activity Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+
-
 Geobacillus stearothermophilus
1.4.1.2 NADH
-
 Geobacillus stearothermophilus
1.4.1.13 NADH no activity with NADH Geobacillus stearothermophilus
1.4.1.13 NADPH
-
 Geobacillus stearothermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.13 0.012
-
 Cibacron blue 3GA
-
 Geobacillus stearothermophilus
1.4.1.13 0.022
-
 L-methionine sulfone
-
 Geobacillus stearothermophilus