EC Number | General Stability | Organism |
---|---|---|
1.4.1.13 | 2-mercaptoethanol, stabilizes | Escherichia coli |
1.4.1.13 | alpha-ketoglutarate stabilizes | Escherichia coli |
1.4.1.13 | alpha-ketoglutarate, L-glutamine, L-glutamate and NADP when present together partial protect against enzyme inactivation | Escherichia coli |
1.4.1.13 | incubation with L-glutamine or L-glutamate results in marked time- and temperature-dependent loss in activity | Escherichia coli |
1.4.1.13 | NADP causes relatively small loss in activity compared with control values | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | ADP | 15% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | ATP | 19% inhibition at 25 mM and 43% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | BaCl2 | 61% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | CaCl2 | 74% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | CdCl2 | produces more than 60% inhibition at 5 mM | Escherichia coli | |
1.4.1.13 | cis-aconitate | 22% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | CoCl2 | produces more than 60% inhibition at 5 mM | Escherichia coli | |
1.4.1.13 | CTP | 9% inhibition at 25 mM and 21% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | D-glutamate | 50% inhibition at concentrations below 7 mM | Escherichia coli | |
1.4.1.13 | D-methionine | 19% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | fumarate | 21% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | glycine | significant inhibitor | Escherichia coli | |
1.4.1.13 | GTP | 16% inhibition at 25 mM and 43% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | iodoacetamide | more than 50% loss in activity in 60 min at 0.5 mM | Escherichia coli | |
1.4.1.13 | isocitrate | 35% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | ITP | 17% inhibition at 25 mM and 29% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | KBr | 43% inhibition at 100 mM | Escherichia coli | |
1.4.1.13 | KCl | 32% inhibition at 100 mM | Escherichia coli | |
1.4.1.13 | KNO3 | 47% inhibition at 100 mM | Escherichia coli | |
1.4.1.13 | L-alanine | significant inhibitor | Escherichia coli | |
1.4.1.13 | L-asparagine | significant inhibitor | Escherichia coli | |
1.4.1.13 | L-aspartate | 50% inhibition at concentrations below 7 mM | Escherichia coli | |
1.4.1.13 | L-cysteine | significant inhibitor | Escherichia coli | |
1.4.1.13 | L-glutamate | 50% inhibition at concentrations between 17 and 35 mM | Escherichia coli | |
1.4.1.13 | L-histidine | significant inhibitor | Escherichia coli | |
1.4.1.13 | L-homoserine | significant inhibitor | Escherichia coli | |
1.4.1.13 | L-malate | 19% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | L-methionine | 50% inhibition at concentrations below 7 mM | Escherichia coli | |
1.4.1.13 | L-serine | significant inhibitor | Escherichia coli | |
1.4.1.13 | MgCl2 | 70% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | MnCl2 | 82% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | NADP+ | 50% inhibition at concentrations below 7 mM | Escherichia coli | |
1.4.1.13 | NiCl2 | produces more than 60% inhibition at 5 mM | Escherichia coli | |
1.4.1.13 | oxaloacetate | - |
Escherichia coli | |
1.4.1.13 | p-chloromercuriphenylsulfonate | more than 50% loss in activity in 60 min at 0.05 mM | Escherichia coli | |
1.4.1.13 | pyruvate | 20% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | succinate | 16% inhibition at 50 mM | Escherichia coli | |
1.4.1.13 | UTP | 17% inhibition at 25 mM and 33% inhibition at 50 mM | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.13 | 0.0073 | - |
alpha-ketoglutarate | - |
Escherichia coli | |
1.4.1.13 | 0.0077 | - |
NADPH | - |
Escherichia coli | |
1.4.1.13 | 0.25 | - |
L-glutamine | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | Iron | iron-sulfur protein | Escherichia coli | |
1.4.1.13 | Iron | contains 38.4 mol of iron | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 53000 | - |
4 * 53000 + 4 * 135000, SDS-PAGE, urea polyacrylamide gel electrophoresis | Escherichia coli |
1.4.1.13 | 135000 | - |
4 * 53000 + 4 * 135000, SDS-PAGE, urea polyacrylamide gel electrophoresis | Escherichia coli |
1.4.1.13 | 800000 | - |
sedimentation equilibrium, gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.13 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.13 | using streptomycin sulfate treatment, ammonium sulfate fractionation, heat treatment, agarose gel filtration and DEAE-cellulose column chromatography | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 26.2 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.13 | -80°C, 20 mM potassium-HEPES, pH 7.2, 2 mM alpha-ketoglutarate, 1 mM EDTA, stable for at least 6 months | Escherichia coli |
1.4.1.13 | 4°C, 20 mM potassium-HEPES, pH 7.2, 2 mM alpha-ketoglutarate, 1 mM EDTA, 5 mM 2-mercaptoethanol, 20% loss of activity, after 1 month | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | highly specific | Escherichia coli | L-glutamate + NADP+ | - |
ir | |
1.4.1.13 | L-glutamine + 2-oxoglutarate + NADPH + H+ | L-glutamine, 2-oxoglutarate and NADPH are all required for catalytic activity | Escherichia coli | L-glutamate + NADP+ | - |
ir | |
1.4.1.13 | additional information | NH4Cl, L-asparagine, D-glutamine, or alkylated glutamine analogues do not substitute for L-glutamine, pyruvate or oxalacetate do not substitute for alpha-ketoglutarate | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.13 | octamer | 4 * 53000 + 4 * 135000, SDS-PAGE, urea polyacrylamide gel electrophoresis | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 7.6 | - |
- |
Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.13 | 6.4 | 9 | at pH 6.4 and 9.0 about 50% of activity maximum | Escherichia coli |
1.4.1.13 | 6.5 | 8.7 | at pH 6.5 and 8.7 about 50% of activity maximum | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.13 | flavin | flavoenzyme | Escherichia coli | |
1.4.1.13 | flavin | contains FAD and FMN | Escherichia coli | |
1.4.1.13 | flavin | ratio FAD: FMN is 1.2 | Escherichia coli | |
1.4.1.13 | flavin | enzyme contains 7.8 mol of flavin | Escherichia coli | |
1.4.1.13 | additional information | purified enzyme contains 30.4 mol of labile sulfide per 800,000 g of protein | Escherichia coli | |
1.4.1.13 | NADH | no activity with NADH | Escherichia coli | |
1.4.1.13 | NADPH | - |
Escherichia coli |