EC Number | General Stability | Organism |
---|---|---|
1.4.1.11 | enzyme is very unstable in crude extracts and to dilution in buffer, purified enzyme is only partially inactivated by dilution in a solution of low ionic strength | Clostridium sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.11 | D-erythro-3,5-diaminohexanoate | non-competitive inhibitor of L-erythro-3,5-diaminohexanoate | Clostridium sp. | |
1.4.1.11 | DL-threo-3,5-diaminohexanoate | partial inhibition by concentrations above 0.3 mM, pH 6.8 | Clostridium sp. | |
1.4.1.11 | NADH | dimeric and tetrameric form of enzyme: product inhibition, competitive inhibitor to NAD+ and uncompetitive inhibitor to 5-amino-3-oxohexanoate | Clostridium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.11 | 0.074 | - |
NADH | pH 7.0, 23°C | Clostridium sp. | |
1.4.1.11 | 0.13 | - |
NAD+ | pH 6.6, 24°C, dimeric form of enzyme | Clostridium sp. | |
1.4.1.11 | 0.18 | - |
L-erythro-3,5-diaminohexanoate | pH 6.8, 24°C, tetrameric form of enzyme, pH 6.8 | Clostridium sp. | |
1.4.1.11 | 0.22 | - |
L-erythro-3,5-diaminohexanoate | pH 6.8, 24°C, tetrameric form of enzyme, above pH 6.8 | Clostridium sp. | |
1.4.1.11 | 0.25 | - |
L-erythro-3,5-diaminohexanoate | pH 6.6, 24°C, dimeric form of enzyme, pH 6.6 | Clostridium sp. | |
1.4.1.11 | 0.26 | - |
5-amino-3-oxohexanoate | pH 7.0, 23°C | Clostridium sp. | |
1.4.1.11 | 0.28 | - |
NAD+ | pH 6.8, 24°C, tetrameric form of enzyme | Clostridium sp. | |
1.4.1.11 | 0.77 | - |
L-erythro-3,5-diaminohexanoate | pH 6.8, 24°C, tetrameric form of enzyme, pH 7.6 and pH 8.9 | Clostridium sp. | |
1.4.1.11 | 140 | - |
NH4+ | pH 7.0, 23°C | Clostridium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.11 | 37000 | - |
2 * 37000, the dimer is about one third as active as the tetramer, SDS-PAGE | Clostridium sp. |
1.4.1.11 | 37000 | - |
4 * 37000, the dimer is about one third as active as the tetramer, SDS-PAGE | Clostridium sp. |
1.4.1.11 | 68000 | - |
dimer, gel filtration | Clostridium sp. |
1.4.1.11 | 135000 | - |
tetramer, gel filtration | Clostridium sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | Clostridium sp. | enzyme is involved in lysine degradation | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
? | |
1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | Clostridium sp. SB4 | enzyme is involved in lysine degradation | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.11 | Clostridium sp. | - |
- |
- |
1.4.1.11 | Clostridium sp. SB4 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.11 | - |
Clostridium sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.11 | 34 | - |
- |
Clostridium sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.11 | 12°C, 100 mM EDTA, pH 6.8, 5 months, 50% loss of activity | Clostridium sp. |
1.4.1.11 | 4°C, suspension in ammonium sulfate, 6 months, no detectable loss of activity | Clostridium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | enzyme is involved in lysine degradation | Clostridium sp. | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
? | |
1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | enzyme is involved in lysine degradation | Clostridium sp. SB4 | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
? | |
1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | the equilibrium of the dehydrogenase reaction is more favorable for amino acid oxidation than are the equilibria of other similar reactions | Clostridium sp. | 5-amino-3-oxohexanoate + NADH + NH4+ | - |
r | |
1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | the equilibrium of the dehydrogenase reaction is more favorable for amino acid oxidation than are the equilibria of other similar reactions | Clostridium sp. SB4 | 5-amino-3-oxohexanoate + NADH + NH4+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.11 | dimer | 2 * 37000, the dimer is about one third as active as the tetramer, SDS-PAGE | Clostridium sp. |
1.4.1.11 | More | the enzyme can exist in at least two forms which have different specific activities. The more active form is favored by a high concentration of enzyme and by the presence of a saturating level of L-erythro-3,5-diaminohexanoate and of high concentrations of various salts of which ammonium chloride, Tris chloride, and sodium EDTA are most effective. The less active form is favored by a low concentration of enzyme and low ionic strength | Clostridium sp. |
1.4.1.11 | tetramer | 4 * 37000, the dimer is about one third as active as the tetramer, SDS-PAGE | Clostridium sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.11 | L-erythro-3,5-diaminohexanoate dehydrogenase | - |
Clostridium sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.11 | 4 | - |
in absence of glycerol and EDTA, half-life is 100 h | Clostridium sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.11 | 6 | 7 | the pH at which the enzyme is most stable depends on the buffer, but generally it is between 6 and 7 | Clostridium sp. |
1.4.1.11 | 7.8 | - |
in the absence of glycerol the enzyme dissociates and inactivates at pH 7.8 or above | Clostridium sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.11 | 3-acetylpyridine-NAD+ | 70% of activity with NAD+, activity with NADP is 1.3% of the activity with NAD+ | Clostridium sp. | |
1.4.1.11 | additional information | activity with NADP+ is 1.3% of the activity with NAD+ | Clostridium sp. | |
1.4.1.11 | NAD+ | NAD+ is most active cofactor | Clostridium sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.11 | 0.004 | - |
NADH | pH 6.8, 24°C, tetrameric form of enzyme | Clostridium sp. | |
1.4.1.11 | 0.5 | - |
D-erythro-3,5-diaminohexanoate | pH 6.7, 24°C | Clostridium sp. | |
1.4.1.11 | 1.2 | - |
DL-threo-3,5-diaminohexanoate | pH 6.7, 24°C | Clostridium sp. | |
1.4.1.11 | 16 | - |
NADH | pH 6.6, 24°C, dimeric form of enzyme | Clostridium sp. |