Any feedback?
Literature summary extracted from
- Aerobic metabolism of 3,5-diaminohexanoate in a Brevibacterium. Purification of 3,5-diaminohexanoate dehydrogenase and degradation of 3-keto-5-aminohexanoate (1973), J. Biol. Chem., 248, 41-49.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.11 | L-erythro-3,5-diaminohexanoate | - |
Brevibacterium L5 |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.11 | 0.75 | - |
L-erythro-3,5-diaminohexanoate | pH 9.3 | Brevibacterium L5 | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.11 | 71000 | - |
gel filtration | Brevibacterium L5 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | Brevibacterium L5 | 3-keto-5-aminohexanoate has the (5S) configuration. First step in the decomposition of 3,5-diaminohexanoate by the aerobic Brevibacterium L5 | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.11 | Brevibacterium L5 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.11 | - |
Brevibacterium L5 |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.4.1.11 | -20°C, 4 months, enzyme in cells extract is stable for 4 months | Brevibacterium L5 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | 3-keto-5-aminohexanoate has the (5S) configuration. First step in the decomposition of 3,5-diaminohexanoate by the aerobic Brevibacterium L5 | Brevibacterium L5 | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
r | |
| 1.4.1.11 | L-erythro-3,5-diaminohexanoate + H2O + NAD+ | 3-keto-5-aminohexanoate has the (5S) configuration | Brevibacterium L5 | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.11 | 9.3 | - |
- |
Brevibacterium L5 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.11 | 8.3 | 10.2 | pH 8.3: about 55% of maximal activity, pH 10.2: about 60% of maximal activity | Brevibacterium L5 |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.11 | 7 | 8.5 | enzyme is most stable between pH 7.0 and 8.5 | Brevibacterium L5 |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.11 | NAD+ | - |
Brevibacterium L5 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
