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Literature summary extracted from

  • Davidson, S.J.; Talalay, P.
    Purification and mechanism of action of a steroid delta-4-5-beta-dehydrogenase (1966), J. Biol. Chem., 241, 906-915.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.99.6 glycerol stabilizes Comamonas testosteroni
1.3.99.6 phenazine methosulfate stabilizes Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.6 32000
-
 gel filtration Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.6 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.6
-
 Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.6 6.5
-
-
 Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
1.3.99.6 4°C, in neutral buffer, 20% glycerol, about 50% loss of activity after 1 month Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.6 17beta-hydroxy-5beta-androstan-3-one + menaphthone acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10 Comamonas testosteroni testosterone + reduced acceptor
-
 ?
1.3.99.6 21-hydroxy-5beta-pregnan-3,20-dione + acceptor acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10 Comamonas testosteroni 21-hydroxy-pregn-4-en-3,20-dione + reduced acceptor
-
 ?
1.3.99.6 5beta-androst-3,17-dione + acceptor acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10 Comamonas testosteroni androst-1,4-dien-3,17-dione + reduced acceptor
-
 ?
1.3.99.6 5beta-androstan-3,17-dione + acceptor acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10 Comamonas testosteroni 4-androsten-3,17-dione + reduced acceptor
-
 ?
1.3.99.6 5beta-androstane-3,17-dione + acceptor acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10 Comamonas testosteroni androst-4-en-3,17-dione + reduced acceptor
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.6 9
-
 above pH 9.0, the rate of DELTA4-5beta-dehydrogenase reaction increases with pH to the limit where accurate measurement can be made, pH 9.0 Comamonas testosteroni