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Literature summary extracted from

  • Battaile, K.P.; Mohsen, A.W.A.; Vockley, J.
    Functional role of the active site glutamate-368 in rat short chain Acyl-CoA dehydrogenase (1996), Biochemistry, 35, 15356-15363.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.1 expression of wild-type, E368G/G247E, E368D, E368Q and E368G mutant enzyme in Escherichia coli Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.1 E368D mutant exhibits enzyme activity Rattus norvegicus
1.3.8.1 E368G inactive enzyme, E368 is responsible for catalytic activity Rattus norvegicus
1.3.8.1 E368G/G247E mutant exhibits enzyme activity Rattus norvegicus
1.3.8.1 E368L inactive enzyme, E368 is responsible for catalytic activity Rattus norvegicus
1.3.8.1 E368Q inactive enzyme, E368 is responsible for catalytic activity Rattus norvegicus
1.3.8.1 E368R inactive enzyme, E368 is responsible for catalytic activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.1 0.0006
-
 butyryl-CoA wild-type enzyme Rattus norvegicus
1.3.8.1 0.0011
-
 octanoyl-CoA E368G/G247E double mutant enzyme Rattus norvegicus
1.3.8.1 0.0015
-
 butyryl-CoA E368D mutant enzyme Rattus norvegicus
1.3.8.1 0.0017
-
 hexanoyl-CoA E368G/G247E double mutant enzyme Rattus norvegicus
1.3.8.1 0.002
-
 butyryl-CoA E368G/G247E double mutant enzyme Rattus norvegicus
1.3.8.1 0.0038
-
 hexanoyl-CoA wild-type enzyme Rattus norvegicus
1.3.8.1 0.005
-
 octanoyl-CoA E368D mutant enzyme Rattus norvegicus
1.3.8.1 0.005
-
 hexanoyl-CoA E368D mutant enzyme Rattus norvegicus
1.3.8.1 0.0081
-
 octanoyl-CoA wild-type enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.1 wild-type enzyme purified, E368Q, E368G and E368L mutant enzyme, partially purified Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.1 0.3
-
 E368G/G247E double mutant enzyme, electron transfer protein as electron acceptor Rattus norvegicus
1.3.8.1 1.9
-
 E368D mutant enzyme, electron transfer protein as electron acceptor Rattus norvegicus
1.3.8.1 3.8
-
 wild-type enzyme, electron transfer protein as electron acceptor Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 octanoyl-CoA + electron transfer protein
-
 Rattus norvegicus 2-octenoyl-CoA + reduced acceptor
-
 ?