BRENDA - Enzyme Database

Studies on the fatty acid oxidization system of animal tissues. III. Butyryl coenzyme A dehydrogenase

Green, D.E.; Mahler, H.R.; J. Biol. Chem. 206, 1-12 (1954) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.8.1
0.014
-
butyryl-CoA
-
Bos taurus
1.3.8.1
0.083
-
ferricytochrome c
-
Bos taurus
1.3.8.1
0.33
-
2,6-dichlorophenolindophenol
-
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.8.1
120000
220000
analytical ultracentrifugation
Bos taurus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.3.8.1
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.3.8.1
-
Bos taurus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.8.1
additional information
-
DELTA D600 = 12.2/min/mg protein
Bos taurus
1.3.8.1
0.77
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.3.8.1
butyryl-CoA + electron acceptor
-
391300
Bos taurus
2-butenoyl-CoA + reduced acceptor
-
391300
Bos taurus
r
1.3.8.1
butyryl-CoA + ferricytochrome c
-
391300
Bos taurus
butenoyl-CoA + reduced acceptor
-
391300
Bos taurus
r
1.3.8.1
butyryl-CoA + pyocyanine + triphenyltetrazolium chloride
-
391300
Bos taurus
2-butenoyl-CoA + reduced acceptor
-
391300
Bos taurus
r
1.3.8.1
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
40% of activity with butyryl-CoA
391300
Bos taurus
2-hexenoyl-CoA + reduced acceptor
-
391300
Bos taurus
?
1.3.8.1
pentanoyl-CoA + 2,6-dichlorophenolindophenol + phenazine ethosulfate
-
391300
Bos taurus
2-pentenoyl-CoA + reduced acceptor
-
391300
Bos taurus
?
1.3.8.1
propionyl-CoA + 2,6-dichlorophenolindophenol
30% of activity with butyryl-CoA
391300
Bos taurus
2-propenoyl-CoA + reduced acceptor
-
391300
Bos taurus
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.8.1
33.3
-
butyryl-CoA
based on an estimated MW of 150000 Da
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.8.1
7
-
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.8.1
6.5
8
42% activity at pH 6.5, 78% at pH 7.5 and 65% at pH 8.0
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.8.1
0.014
-
butyryl-CoA
-
Bos taurus
1.3.8.1
0.083
-
ferricytochrome c
-
Bos taurus
1.3.8.1
0.33
-
2,6-dichlorophenolindophenol
-
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.8.1
120000
220000
analytical ultracentrifugation
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.8.1
-
Bos taurus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.8.1
additional information
-
DELTA D600 = 12.2/min/mg protein
Bos taurus
1.3.8.1
0.77
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.3.8.1
butyryl-CoA + electron acceptor
-
391300
Bos taurus
2-butenoyl-CoA + reduced acceptor
-
391300
Bos taurus
r
1.3.8.1
butyryl-CoA + ferricytochrome c
-
391300
Bos taurus
butenoyl-CoA + reduced acceptor
-
391300
Bos taurus
r
1.3.8.1
butyryl-CoA + pyocyanine + triphenyltetrazolium chloride
-
391300
Bos taurus
2-butenoyl-CoA + reduced acceptor
-
391300
Bos taurus
r
1.3.8.1
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
40% of activity with butyryl-CoA
391300
Bos taurus
2-hexenoyl-CoA + reduced acceptor
-
391300
Bos taurus
?
1.3.8.1
pentanoyl-CoA + 2,6-dichlorophenolindophenol + phenazine ethosulfate
-
391300
Bos taurus
2-pentenoyl-CoA + reduced acceptor
-
391300
Bos taurus
?
1.3.8.1
propionyl-CoA + 2,6-dichlorophenolindophenol
30% of activity with butyryl-CoA
391300
Bos taurus
2-propenoyl-CoA + reduced acceptor
-
391300
Bos taurus
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.8.1
33.3
-
butyryl-CoA
based on an estimated MW of 150000 Da
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.8.1
7
-
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.8.1
6.5
8
42% activity at pH 6.5, 78% at pH 7.5 and 65% at pH 8.0
Bos taurus