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Literature summary extracted from

  • Williamson, G.; Engel, P.C.
    Butyryl-CoA dehydrogenase from Megasphaera elsdenii. Specificity of the catalytic reaction (1984), Biochem. J., 218, 521-529.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.1 0.015
-
butyryl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.016
-
pentanoyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.053
-
hexanoyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.082
-
cyclohexanecarboxyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.095
-
cyclopentanecarboxyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.097
-
4-methylpentanoyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.119
-
cyclobutanecarboxyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.13 0.15 propionyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.161
-
2-methylpropionyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.198
-
cycloheptanecarboxyl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.213
-
S-2-methylbutyryl-CoA
-
Megasphaera elsdenii
1.3.8.1 0.59
-
phenazine ethosulfate phenazine ethosulfate as intermediate electron carrier Megasphaera elsdenii
1.3.8.1 0.8
-
phenazine methosulfate
-
Megasphaera elsdenii
1.3.8.1 0.83
-
phenazine methosulfate phenazine methosulfate as intermediate electron carrier Megasphaera elsdenii

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Megasphaera elsdenii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 (S)-2-methylbutyryl-CoA + phenazine methosulfate + dichloroindophenol 6.0% of activity with butyryl-CoA Megasphaera elsdenii (S)-2-methyl-2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 2-methylpropionyl-CoA + phenazine methosulfate + dichloroindophenol 1.5% of activity with butyryl-CoA Megasphaera elsdenii 2-methylpropenoyl-CoA + reduced acceptor
-
?
1.3.8.1 2-propenoyl-CoA + reduced electron transfer flavoprotein
-
Megasphaera elsdenii propanoyl-CoA + electron transfer protein
-
?
1.3.8.1 4-methylpentanoyl-CoA + phenazine methosulfate + dichloroindophenol 18.0% of activity with butyryl-CoA Megasphaera elsdenii 4-methyl-2-pentenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor intermediate electron acceptors phenazine methosulfate or phenazine ethosulfate Megasphaera elsdenii 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor intermediate electron acceptors phenazine methosulfate or Meldola' s Blue (i.e. 8-dimethylamino-2,3-benzophenoxazinium chloride) Megasphaera elsdenii 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor terminal electron acceptor 2,6-dichlorophenol-indophenol Megasphaera elsdenii 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor terminal electron acceptor iodonitrotetrazolium chloride Megasphaera elsdenii 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor intermediate electron acceptor phenazine methosulfate Megasphaera elsdenii 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 cyclobutanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol 1.0% of activity with butyryl-CoA Megasphaera elsdenii cyclo-2-butenecarboxyl-CoA + reduced acceptor
-
?
1.3.8.1 cycloheptanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol 3.2% of activity with butyryl-CoA Megasphaera elsdenii cyclo-2-heptenecarboxyl-CoA + reduced acceptor
-
?
1.3.8.1 cyclohexanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol 6.7% of activity with butyryl-CoA Megasphaera elsdenii cyclo-2-hexenecarboxyl-CoA + reduced acceptor
-
?
1.3.8.1 cyclopentanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol 2.1% of activity with butyryl-CoA Megasphaera elsdenii cyclo-2-pentenecarboxyl-CoA + reduced acceptor
-
?
1.3.8.1 hexanoyl-CoA + phenazine methosulfate + dichloroindophenol 4.2% of activity with butyryl-coA Megasphaera elsdenii 2-hexenoyl-CoA + reduced acceptor
-
?
1.3.8.1 hexanoyl-CoA + phenazine methosulfate + dichloroindophenol acceptor Meldola's Blue and iodonitrotetrazolium chloride Megasphaera elsdenii 2-hexenoyl-CoA + reduced acceptor
-
?
1.3.8.1 pentanoyl-CoA + phenazine methosulfate 45% of activity with butyryl-CoA, 2,6-dichlorophenolindophenol as final electron acceptor Megasphaera elsdenii 2-pentenoyl-CoA + reduced acceptor
-
?
1.3.8.1 pentanoyl-CoA + phenazine methosulfate pentanoyl-CoA i.e. valeryl-CoA Megasphaera elsdenii 2-pentenoyl-CoA + reduced acceptor
-
?
1.3.8.1 S-butyrylpantetheine + phenazine ethosulfate + dichloroindophenol 51.0% of activity with butyryl-CoA Megasphaera elsdenii S-but-2-eneoylpantetheine + reduced acceptor
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.1 8.43
-
butyryl-CoA phenazine ethosulfate as intermediate electron carrier Megasphaera elsdenii
1.3.8.1 20.7
-
butyryl-CoA
-
Megasphaera elsdenii
1.3.8.1 20.8
-
butyryl-CoA phenazine methosulfate as intermediate electron carrier Megasphaera elsdenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.1 8
-
-
Megasphaera elsdenii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.8.1 7.5 9 57% activity at pH 7.6, 72% activity at pH 9 Megasphaera elsdenii