BRENDA - Enzyme Database

Purification and characterization of short-chain, medium-chain, and long-chain acyl-CoA dehydrogenases from rat liver mitochondria. Isolation of the holo- and apoenzymes and conversion of the apoenzyme to the holoenzyme

Ikeda, Y.; Okamura-Ikeda, K.; Tanaka, K.; J. Biol. Chem. 260, 1311-1325 (1985)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.8.1
2-butenoyl-CoA
competitive product inhibition
Rattus norvegicus
1.3.8.1
palmitoyl-CoA
-
Rattus norvegicus
1.3.8.7
2-hexadecenoyl-CoA
competitive, long-chain acyl-CoA dehdrogenase
Rattus norvegicus
1.3.8.7
additional information
not inhibited by saturated and 2,3-unsaturated branched chain acyl-CoA compounds
Rattus norvegicus
1.3.8.7
octenoyl-CoA
competitive, medium-chain acyl-CoA dehydrogenase
Rattus norvegicus
1.3.8.8
2-hexadecenoyl-CoA
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.8.1
0.0107
-
butyryl-CoA
-
Rattus norvegicus
1.3.8.1
0.0329
-
pentanoyl-CoA
-
Rattus norvegicus
1.3.8.1
0.285
-
hexanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.004
-
octanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.0045
-
electron transfer flavoprotein
-
Rattus norvegicus
1.3.8.7
0.0054
-
decanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.0057
-
dodecanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.0094
-
hexanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.0396
-
pentanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.135
-
butanoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0025
-
FAD
-
Rattus norvegicus
1.3.8.8
0.0025
-
palmitoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0054
-
stearoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0065
-
oleoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0074
-
tetradecanoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0083
-
electron-transfer flavoprotein
-
Rattus norvegicus
1.3.8.8
0.009
-
dodecanoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0243
-
decanoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.123
-
octanoyl-CoA
-
Rattus norvegicus
1.3.8.8
1
-
phenazine methosulfate
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.8.1
mitochondrion
-
Rattus norvegicus
5739
-
1.3.8.8
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.8.1
41000
-
4 * 41000, SDS-PAGE
Rattus norvegicus
1.3.8.1
160000
-
gel filtration
Rattus norvegicus
1.3.8.7
45000
-
4 * 45000, SDS-PAGE
Rattus norvegicus
1.3.8.7
180000
-
gel filtration
Rattus norvegicus
1.3.8.8
180000
-
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.3.8.1
Rattus norvegicus
-
-
-
1.3.8.7
Rattus norvegicus
-
-
-
1.3.8.8
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.3.8.1
-
Rattus norvegicus
1.3.8.7
-
Rattus norvegicus
1.3.8.8
40-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite, matrex gel blue A, agarose-hexane-CoA, bio-gel A-0.5m
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.8.1
liver
-
Rattus norvegicus
-
1.3.8.7
liver
-
Rattus norvegicus
-
1.3.8.8
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.3.8.1
10.6
-
-
Rattus norvegicus
1.3.8.7
1.96
-
long-chain acyl-CoA dehydrogenase
Rattus norvegicus
1.3.8.7
10
10.2
-
Rattus norvegicus
1.3.8.7
11.9
-
medium-chain acyl-CoA dehydrogenase
Rattus norvegicus
1.3.8.8
0.06
-
purified holoenzyme, arachidonoyl-CoA
Rattus norvegicus
1.3.8.8
0.1
-
purified holoenzyme, erucoyl-CoA
Rattus norvegicus
1.3.8.8
0.3
-
purified holoenzyme, docosanoyl-CoA
Rattus norvegicus
1.3.8.8
0.38
-
purified holoenzyme, linolenoyl-CoA
Rattus norvegicus
1.3.8.8
0.7
-
purified holoenzyme, octanoyl-CoA
Rattus norvegicus
1.3.8.8
0.72
-
purified holoenzyme, linoleoyl-CoA
Rattus norvegicus
1.3.8.8
0.9
-
purified holoenzyme, eicosanoyl-CoA
Rattus norvegicus
1.3.8.8
1.13
-
purified holoenzyme, oleoyl-CoA
Rattus norvegicus
1.3.8.8
1.5
-
purified holoenzyme, decanoyl-CoA
Rattus norvegicus
1.3.8.8
1.51
-
purified holoenzyme, palmitoleoyl-CoA
Rattus norvegicus
1.3.8.8
1.52
-
purified apoenzyme, palmitoyl-CoA
Rattus norvegicus
1.3.8.8
1.6
-
purified holoenzyme, stearoyl-CoA
Rattus norvegicus
1.3.8.8
1.8
-
purified holoenzyme, dodecanoyl-CoA
Rattus norvegicus
1.3.8.8
1.96
-
purified holoenzyme, palmitoyl-CoA
Rattus norvegicus
1.3.8.8
2.1
-
purified holoenzyme, tetradecanoyl-CoA
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
1.3.8.1
-20░C, 10 mM KH2PO4, pH 8.0, 0.2 M NaCl, 50% glycerol, at least 3 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein
391272
Rattus norvegicus
2-butenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.1
butyryl-CoA + electron acceptor
terminal electron acceptor 2,6-dichlorophenol-indophenol
391272
Rattus norvegicus
2-butenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptor phenazine methosulfate
391272
Rattus norvegicus
2-butenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.1
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
-
391272
Rattus norvegicus
2-hexenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.1
pentanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-pentenoyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
acyl-CoA + acceptor
electron acceptor: phenazine methosulfate
391272
Rattus norvegicus
2,3-dehydroacyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
acyl-CoA + acceptor
intermediary electron acceptor: 2,6-dichloroindophenol
391272
Rattus norvegicus
2,3-dehydroacyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
acyl-CoA + acceptor
final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue
391272
Rattus norvegicus
2,3-dehydroacyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
butanoyl-CoA + acceptor
-
391272
Rattus norvegicus
crotonyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
dodecanoyl-CoA + acceptor
-
391272
Rattus norvegicus
2-dodecenoyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
best substrate for medium-chain acyl-CoA dehydrogenase
391272
Rattus norvegicus
2-octenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
no activity with tetradecanoyl-CoA and hexadecanoyl-CoA
391272
Rattus norvegicus
2-octenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
n-tetradecanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
best substrate for long-chain acyl-CoA dehydrogenase
391272
Rattus norvegicus
2-tetradecenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
pentanoyl-CoA + acceptor
-
391272
Rattus norvegicus
2-pentenoyl-CoA + reduced acceptor
-
-
-
?
1.3.8.8
arachidonoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,5Z,8Z,11Z,14Z)-2,5,8,11,14-eicosapentaenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
decanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-decenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
decanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E)-2-decenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
docosanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-docosenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
docosanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-docosenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
dodecanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-dodecenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
dodecanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E)-2-dodecenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
eicosanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
trans-2-eicosenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
eicosanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
trans-2-eicosenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
erucoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,13Z)-2,13-docosadienoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
linolenoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,6Z,9Z,12Z)2,6,9,12-octadecatetraenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
linoleoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,9Z,12Z)-2,9,12-octadecatrienoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
octanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-octenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
octanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-octenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
oleoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,9Z)-2,9-octadecadienoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
palmitoleoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,9Z)-2,9-hexadecadienoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
palmitoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
palmitoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-hexadecenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
stearoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-octadecenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
stearoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-octadecenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
tetradecanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
tetradecanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-tetradecenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.8.1
tetramer
4 * 41000, SDS-PAGE
Rattus norvegicus
1.3.8.7
tetramer
4 * 45000, SDS-PAGE
Rattus norvegicus
1.3.8.8
tetramer
4 * 38000-45200, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.8.7
6
-
tetradecanoyl-CoA
long-chain acyl-CoA dehydrogenase
Rattus norvegicus
1.3.8.7
31.2
-
hexanoyl-CoA
medium-chain acyl-CoA dehydrogenase
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.3.8.7
5.5
8.7
-
Rattus norvegicus
1.3.8.8
5.5
8.7
stable for at least 10 min at 37░C
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.8.1
FAD
1 mol per mol of subunit
Rattus norvegicus
1.3.8.7
FAD
1 mol per mol of subunit
Rattus norvegicus
1.3.8.8
FAD
-
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.8.1
0.0102
-
2-butenoyl-CoA
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.8.1
FAD
1 mol per mol of subunit
Rattus norvegicus
1.3.8.7
FAD
1 mol per mol of subunit
Rattus norvegicus
1.3.8.8
FAD
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.8.1
2-butenoyl-CoA
competitive product inhibition
Rattus norvegicus
1.3.8.1
palmitoyl-CoA
-
Rattus norvegicus
1.3.8.7
2-hexadecenoyl-CoA
competitive, long-chain acyl-CoA dehdrogenase
Rattus norvegicus
1.3.8.7
additional information
not inhibited by saturated and 2,3-unsaturated branched chain acyl-CoA compounds
Rattus norvegicus
1.3.8.7
octenoyl-CoA
competitive, medium-chain acyl-CoA dehydrogenase
Rattus norvegicus
1.3.8.8
2-hexadecenoyl-CoA
-
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.3.8.1
0.0102
-
2-butenoyl-CoA
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.8.1
0.0107
-
butyryl-CoA
-
Rattus norvegicus
1.3.8.1
0.0329
-
pentanoyl-CoA
-
Rattus norvegicus
1.3.8.1
0.285
-
hexanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.004
-
octanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.0045
-
electron transfer flavoprotein
-
Rattus norvegicus
1.3.8.7
0.0054
-
decanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.0057
-
dodecanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.0094
-
hexanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.0396
-
pentanoyl-CoA
-
Rattus norvegicus
1.3.8.7
0.135
-
butanoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0025
-
FAD
-
Rattus norvegicus
1.3.8.8
0.0025
-
palmitoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0054
-
stearoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0065
-
oleoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0074
-
tetradecanoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0083
-
electron-transfer flavoprotein
-
Rattus norvegicus
1.3.8.8
0.009
-
dodecanoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.0243
-
decanoyl-CoA
-
Rattus norvegicus
1.3.8.8
0.123
-
octanoyl-CoA
-
Rattus norvegicus
1.3.8.8
1
-
phenazine methosulfate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.8.1
mitochondrion
-
Rattus norvegicus
5739
-
1.3.8.8
mitochondrion
-
Rattus norvegicus
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.8.1
41000
-
4 * 41000, SDS-PAGE
Rattus norvegicus
1.3.8.1
160000
-
gel filtration
Rattus norvegicus
1.3.8.7
45000
-
4 * 45000, SDS-PAGE
Rattus norvegicus
1.3.8.7
180000
-
gel filtration
Rattus norvegicus
1.3.8.8
180000
-
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.8.1
-
Rattus norvegicus
1.3.8.7
-
Rattus norvegicus
1.3.8.8
40-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite, matrex gel blue A, agarose-hexane-CoA, bio-gel A-0.5m
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.8.1
liver
-
Rattus norvegicus
-
1.3.8.7
liver
-
Rattus norvegicus
-
1.3.8.8
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.3.8.1
10.6
-
-
Rattus norvegicus
1.3.8.7
1.96
-
long-chain acyl-CoA dehydrogenase
Rattus norvegicus
1.3.8.7
10
10.2
-
Rattus norvegicus
1.3.8.7
11.9
-
medium-chain acyl-CoA dehydrogenase
Rattus norvegicus
1.3.8.8
0.06
-
purified holoenzyme, arachidonoyl-CoA
Rattus norvegicus
1.3.8.8
0.1
-
purified holoenzyme, erucoyl-CoA
Rattus norvegicus
1.3.8.8
0.3
-
purified holoenzyme, docosanoyl-CoA
Rattus norvegicus
1.3.8.8
0.38
-
purified holoenzyme, linolenoyl-CoA
Rattus norvegicus
1.3.8.8
0.7
-
purified holoenzyme, octanoyl-CoA
Rattus norvegicus
1.3.8.8
0.72
-
purified holoenzyme, linoleoyl-CoA
Rattus norvegicus
1.3.8.8
0.9
-
purified holoenzyme, eicosanoyl-CoA
Rattus norvegicus
1.3.8.8
1.13
-
purified holoenzyme, oleoyl-CoA
Rattus norvegicus
1.3.8.8
1.5
-
purified holoenzyme, decanoyl-CoA
Rattus norvegicus
1.3.8.8
1.51
-
purified holoenzyme, palmitoleoyl-CoA
Rattus norvegicus
1.3.8.8
1.52
-
purified apoenzyme, palmitoyl-CoA
Rattus norvegicus
1.3.8.8
1.6
-
purified holoenzyme, stearoyl-CoA
Rattus norvegicus
1.3.8.8
1.8
-
purified holoenzyme, dodecanoyl-CoA
Rattus norvegicus
1.3.8.8
1.96
-
purified holoenzyme, palmitoyl-CoA
Rattus norvegicus
1.3.8.8
2.1
-
purified holoenzyme, tetradecanoyl-CoA
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.3.8.1
-20░C, 10 mM KH2PO4, pH 8.0, 0.2 M NaCl, 50% glycerol, at least 3 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein
391272
Rattus norvegicus
2-butenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.1
butyryl-CoA + electron acceptor
terminal electron acceptor 2,6-dichlorophenol-indophenol
391272
Rattus norvegicus
2-butenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptor phenazine methosulfate
391272
Rattus norvegicus
2-butenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.1
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
-
391272
Rattus norvegicus
2-hexenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.1
pentanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-pentenoyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
acyl-CoA + acceptor
electron acceptor: phenazine methosulfate
391272
Rattus norvegicus
2,3-dehydroacyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
acyl-CoA + acceptor
intermediary electron acceptor: 2,6-dichloroindophenol
391272
Rattus norvegicus
2,3-dehydroacyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
acyl-CoA + acceptor
final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue
391272
Rattus norvegicus
2,3-dehydroacyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
butanoyl-CoA + acceptor
-
391272
Rattus norvegicus
crotonyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
dodecanoyl-CoA + acceptor
-
391272
Rattus norvegicus
2-dodecenoyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
best substrate for medium-chain acyl-CoA dehydrogenase
391272
Rattus norvegicus
2-octenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
no activity with tetradecanoyl-CoA and hexadecanoyl-CoA
391272
Rattus norvegicus
2-octenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
n-tetradecanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
best substrate for long-chain acyl-CoA dehydrogenase
391272
Rattus norvegicus
2-tetradecenoyl-CoA + reduced acceptor
-
391272
Rattus norvegicus
?
1.3.8.7
pentanoyl-CoA + acceptor
-
391272
Rattus norvegicus
2-pentenoyl-CoA + reduced acceptor
-
-
-
?
1.3.8.8
arachidonoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,5Z,8Z,11Z,14Z)-2,5,8,11,14-eicosapentaenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
decanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-decenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
decanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E)-2-decenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
docosanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-docosenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
docosanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-docosenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
dodecanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-dodecenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
dodecanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E)-2-dodecenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
eicosanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
trans-2-eicosenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
eicosanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
trans-2-eicosenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
erucoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,13Z)-2,13-docosadienoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
linolenoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,6Z,9Z,12Z)2,6,9,12-octadecatetraenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
linoleoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,9Z,12Z)-2,9,12-octadecatrienoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
octanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-octenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
octanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-octenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
oleoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,9Z)-2,9-octadecadienoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
palmitoleoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
(2E,9Z)-2,9-hexadecadienoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
palmitoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
palmitoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-hexadecenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
stearoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-octadecenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
stearoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-octadecenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
1.3.8.8
tetradecanoyl-CoA + electron-transfer flavoprotein
-
391272
Rattus norvegicus
2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein
-
391272
Rattus norvegicus
?
1.3.8.8
tetradecanoyl-CoA + phenazine methosulfate
-
391272
Rattus norvegicus
2-tetradecenoyl-CoA + reduced phenazine methosulfate
-
391272
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.8.1
tetramer
4 * 41000, SDS-PAGE
Rattus norvegicus
1.3.8.7
tetramer
4 * 45000, SDS-PAGE
Rattus norvegicus
1.3.8.8
tetramer
4 * 38000-45200, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.8.7
6
-
tetradecanoyl-CoA
long-chain acyl-CoA dehydrogenase
Rattus norvegicus
1.3.8.7
31.2
-
hexanoyl-CoA
medium-chain acyl-CoA dehydrogenase
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.3.8.7
5.5
8.7
-
Rattus norvegicus
1.3.8.8
5.5
8.7
stable for at least 10 min at 37░C
Rattus norvegicus