EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.1 | 2-butenoyl-CoA | competitive product inhibition | Rattus norvegicus | |
1.3.8.1 | palmitoyl-CoA | - |
Rattus norvegicus | |
1.3.8.7 | 2-hexadecenoyl-CoA | competitive, long-chain acyl-CoA dehdrogenase | Rattus norvegicus | |
1.3.8.7 | additional information | not inhibited by saturated and 2,3-unsaturated branched chain acyl-CoA compounds | Rattus norvegicus | |
1.3.8.7 | octenoyl-CoA | competitive, medium-chain acyl-CoA dehydrogenase | Rattus norvegicus | |
1.3.8.8 | 2-hexadecenoyl-CoA | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.1 | 0.0107 | - |
butyryl-CoA | - |
Rattus norvegicus | |
1.3.8.1 | 0.0329 | - |
pentanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.1 | 0.285 | - |
hexanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.7 | 0.004 | - |
octanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.7 | 0.0045 | - |
electron transfer flavoprotein | - |
Rattus norvegicus | |
1.3.8.7 | 0.0054 | - |
decanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.7 | 0.0057 | - |
dodecanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.7 | 0.0094 | - |
hexanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.7 | 0.0396 | - |
pentanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.7 | 0.135 | - |
butanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.8 | 0.0025 | - |
FAD | - |
Rattus norvegicus | |
1.3.8.8 | 0.0025 | - |
palmitoyl-CoA | - |
Rattus norvegicus | |
1.3.8.8 | 0.0054 | - |
stearoyl-CoA | - |
Rattus norvegicus | |
1.3.8.8 | 0.0065 | - |
oleoyl-CoA | - |
Rattus norvegicus | |
1.3.8.8 | 0.0074 | - |
tetradecanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.8 | 0.0083 | - |
electron-transfer flavoprotein | - |
Rattus norvegicus | |
1.3.8.8 | 0.009 | - |
dodecanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.8 | 0.0243 | - |
decanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.8 | 0.123 | - |
octanoyl-CoA | - |
Rattus norvegicus | |
1.3.8.8 | 1 | - |
phenazine methosulfate | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.8.1 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
1.3.8.8 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.8.1 | 41000 | - |
4 * 41000, SDS-PAGE | Rattus norvegicus |
1.3.8.1 | 160000 | - |
gel filtration | Rattus norvegicus |
1.3.8.7 | 45000 | - |
4 * 45000, SDS-PAGE | Rattus norvegicus |
1.3.8.7 | 180000 | - |
gel filtration | Rattus norvegicus |
1.3.8.8 | 180000 | - |
gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.1 | Rattus norvegicus | - |
- |
- |
1.3.8.7 | Rattus norvegicus | - |
- |
- |
1.3.8.8 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.1 | - |
Rattus norvegicus |
1.3.8.7 | - |
Rattus norvegicus |
1.3.8.8 | 40-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite, matrex gel blue A, agarose-hexane-CoA, bio-gel A-0.5m | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.8.1 | liver | - |
Rattus norvegicus | - |
1.3.8.7 | liver | - |
Rattus norvegicus | - |
1.3.8.8 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.8.1 | 10.6 | - |
- |
Rattus norvegicus |
1.3.8.7 | 1.96 | - |
long-chain acyl-CoA dehydrogenase | Rattus norvegicus |
1.3.8.7 | 10 | 10.2 | - |
Rattus norvegicus |
1.3.8.7 | 11.9 | - |
medium-chain acyl-CoA dehydrogenase | Rattus norvegicus |
1.3.8.8 | 0.06 | - |
purified holoenzyme, arachidonoyl-CoA | Rattus norvegicus |
1.3.8.8 | 0.1 | - |
purified holoenzyme, erucoyl-CoA | Rattus norvegicus |
1.3.8.8 | 0.3 | - |
purified holoenzyme, docosanoyl-CoA | Rattus norvegicus |
1.3.8.8 | 0.38 | - |
purified holoenzyme, linolenoyl-CoA | Rattus norvegicus |
1.3.8.8 | 0.7 | - |
purified holoenzyme, octanoyl-CoA | Rattus norvegicus |
1.3.8.8 | 0.72 | - |
purified holoenzyme, linoleoyl-CoA | Rattus norvegicus |
1.3.8.8 | 0.9 | - |
purified holoenzyme, eicosanoyl-CoA | Rattus norvegicus |
1.3.8.8 | 1.13 | - |
purified holoenzyme, oleoyl-CoA | Rattus norvegicus |
1.3.8.8 | 1.5 | - |
purified holoenzyme, decanoyl-CoA | Rattus norvegicus |
1.3.8.8 | 1.51 | - |
purified holoenzyme, palmitoleoyl-CoA | Rattus norvegicus |
1.3.8.8 | 1.52 | - |
purified apoenzyme, palmitoyl-CoA | Rattus norvegicus |
1.3.8.8 | 1.6 | - |
purified holoenzyme, stearoyl-CoA | Rattus norvegicus |
1.3.8.8 | 1.8 | - |
purified holoenzyme, dodecanoyl-CoA | Rattus norvegicus |
1.3.8.8 | 1.96 | - |
purified holoenzyme, palmitoyl-CoA | Rattus norvegicus |
1.3.8.8 | 2.1 | - |
purified holoenzyme, tetradecanoyl-CoA | Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.8.1 | -20°C, 10 mM KH2PO4, pH 8.0, 0.2 M NaCl, 50% glycerol, at least 3 months | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.1 | butyryl-CoA + electron acceptor | intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein | Rattus norvegicus | 2-butenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | butyryl-CoA + electron acceptor | terminal electron acceptor 2,6-dichlorophenol-indophenol | Rattus norvegicus | 2-butenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | butyryl-CoA + electron acceptor | intermediate electron acceptor phenazine methosulfate | Rattus norvegicus | 2-butenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | hexanoyl-CoA + phenazine methosulfate + dichloroindophenol | - |
Rattus norvegicus | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.1 | pentanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | acyl-CoA + acceptor | electron acceptor: phenazine methosulfate | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | acyl-CoA + acceptor | intermediary electron acceptor: 2,6-dichloroindophenol | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | acyl-CoA + acceptor | final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | butanoyl-CoA + acceptor | - |
Rattus norvegicus | crotonyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | dodecanoyl-CoA + acceptor | - |
Rattus norvegicus | 2-dodecenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | best substrate for medium-chain acyl-CoA dehydrogenase | Rattus norvegicus | 2-octenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | no activity with tetradecanoyl-CoA and hexadecanoyl-CoA | Rattus norvegicus | 2-octenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | n-tetradecanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | best substrate for long-chain acyl-CoA dehydrogenase | Rattus norvegicus | 2-tetradecenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.7 | pentanoyl-CoA + acceptor | - |
Rattus norvegicus | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
1.3.8.8 | arachidonoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,5Z,8Z,11Z,14Z)-2,5,8,11,14-eicosapentaenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | decanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-decenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.8 | decanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E)-2-decenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | docosanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-docosenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.8 | docosanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-docosenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | dodecanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-dodecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.8 | dodecanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E)-2-dodecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | eicosanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | trans-2-eicosenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.8 | eicosanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | trans-2-eicosenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | erucoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,13Z)-2,13-docosadienoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | linolenoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,6Z,9Z,12Z)2,6,9,12-octadecatetraenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | linoleoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,9Z,12Z)-2,9,12-octadecatrienoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | octanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-octenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.8 | octanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-octenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | oleoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,9Z)-2,9-octadecadienoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | palmitoleoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,9Z)-2,9-hexadecadienoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | palmitoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.8 | palmitoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-hexadecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | stearoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-octadecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.8 | stearoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-octadecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
1.3.8.8 | tetradecanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.8 | tetradecanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-tetradecenoyl-CoA + reduced phenazine methosulfate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.1 | tetramer | 4 * 41000, SDS-PAGE | Rattus norvegicus |
1.3.8.7 | tetramer | 4 * 45000, SDS-PAGE | Rattus norvegicus |
1.3.8.8 | tetramer | 4 * 38000-45200, SDS-PAGE | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.7 | 6 | - |
tetradecanoyl-CoA | long-chain acyl-CoA dehydrogenase | Rattus norvegicus | |
1.3.8.7 | 31.2 | - |
hexanoyl-CoA | medium-chain acyl-CoA dehydrogenase | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.3.8.7 | 5.5 | 8.7 | - |
Rattus norvegicus |
1.3.8.8 | 5.5 | 8.7 | stable for at least 10 min at 37°C | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.1 | FAD | 1 mol per mol of subunit | Rattus norvegicus | |
1.3.8.7 | FAD | 1 mol per mol of subunit | Rattus norvegicus | |
1.3.8.8 | FAD | - |
Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.1 | 0.0102 | - |
2-butenoyl-CoA | - |
Rattus norvegicus |