Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ikeda, Y.; Okamura-Ikeda, K.; Tanaka, K.
    Purification and characterization of short-chain, medium-chain, and long-chain acyl-CoA dehydrogenases from rat liver mitochondria. Isolation of the holo- and apoenzymes and conversion of the apoenzyme to the holoenzyme (1985), J. Biol. Chem., 260, 1311-1325.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.8.1 2-butenoyl-CoA competitive product inhibition Rattus norvegicus
1.3.8.1 palmitoyl-CoA
-
Rattus norvegicus
1.3.8.7 2-hexadecenoyl-CoA competitive, long-chain acyl-CoA dehdrogenase Rattus norvegicus
1.3.8.7 additional information not inhibited by saturated and 2,3-unsaturated branched chain acyl-CoA compounds Rattus norvegicus
1.3.8.7 octenoyl-CoA competitive, medium-chain acyl-CoA dehydrogenase Rattus norvegicus
1.3.8.8 2-hexadecenoyl-CoA
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.1 0.0107
-
butyryl-CoA
-
Rattus norvegicus
1.3.8.1 0.0329
-
pentanoyl-CoA
-
Rattus norvegicus
1.3.8.1 0.285
-
hexanoyl-CoA
-
Rattus norvegicus
1.3.8.7 0.004
-
octanoyl-CoA
-
Rattus norvegicus
1.3.8.7 0.0045
-
electron transfer flavoprotein
-
Rattus norvegicus
1.3.8.7 0.0054
-
decanoyl-CoA
-
Rattus norvegicus
1.3.8.7 0.0057
-
dodecanoyl-CoA
-
Rattus norvegicus
1.3.8.7 0.0094
-
hexanoyl-CoA
-
Rattus norvegicus
1.3.8.7 0.0396
-
pentanoyl-CoA
-
Rattus norvegicus
1.3.8.7 0.135
-
butanoyl-CoA
-
Rattus norvegicus
1.3.8.8 0.0025
-
FAD
-
Rattus norvegicus
1.3.8.8 0.0025
-
palmitoyl-CoA
-
Rattus norvegicus
1.3.8.8 0.0054
-
stearoyl-CoA
-
Rattus norvegicus
1.3.8.8 0.0065
-
oleoyl-CoA
-
Rattus norvegicus
1.3.8.8 0.0074
-
tetradecanoyl-CoA
-
Rattus norvegicus
1.3.8.8 0.0083
-
electron-transfer flavoprotein
-
Rattus norvegicus
1.3.8.8 0.009
-
dodecanoyl-CoA
-
Rattus norvegicus
1.3.8.8 0.0243
-
decanoyl-CoA
-
Rattus norvegicus
1.3.8.8 0.123
-
octanoyl-CoA
-
Rattus norvegicus
1.3.8.8 1
-
phenazine methosulfate
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.1 mitochondrion
-
Rattus norvegicus 5739
-
1.3.8.8 mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.1 41000
-
4 * 41000, SDS-PAGE Rattus norvegicus
1.3.8.1 160000
-
gel filtration Rattus norvegicus
1.3.8.7 45000
-
4 * 45000, SDS-PAGE Rattus norvegicus
1.3.8.7 180000
-
gel filtration Rattus norvegicus
1.3.8.8 180000
-
gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Rattus norvegicus
-
-
-
1.3.8.7 Rattus norvegicus
-
-
-
1.3.8.8 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.1
-
Rattus norvegicus
1.3.8.7
-
Rattus norvegicus
1.3.8.8 40-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite, matrex gel blue A, agarose-hexane-CoA, bio-gel A-0.5m Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.1 liver
-
Rattus norvegicus
-
1.3.8.7 liver
-
Rattus norvegicus
-
1.3.8.8 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.1 10.6
-
-
Rattus norvegicus
1.3.8.7 1.96
-
long-chain acyl-CoA dehydrogenase Rattus norvegicus
1.3.8.7 10 10.2
-
Rattus norvegicus
1.3.8.7 11.9
-
medium-chain acyl-CoA dehydrogenase Rattus norvegicus
1.3.8.8 0.06
-
purified holoenzyme, arachidonoyl-CoA Rattus norvegicus
1.3.8.8 0.1
-
purified holoenzyme, erucoyl-CoA Rattus norvegicus
1.3.8.8 0.3
-
purified holoenzyme, docosanoyl-CoA Rattus norvegicus
1.3.8.8 0.38
-
purified holoenzyme, linolenoyl-CoA Rattus norvegicus
1.3.8.8 0.7
-
purified holoenzyme, octanoyl-CoA Rattus norvegicus
1.3.8.8 0.72
-
purified holoenzyme, linoleoyl-CoA Rattus norvegicus
1.3.8.8 0.9
-
purified holoenzyme, eicosanoyl-CoA Rattus norvegicus
1.3.8.8 1.13
-
purified holoenzyme, oleoyl-CoA Rattus norvegicus
1.3.8.8 1.5
-
purified holoenzyme, decanoyl-CoA Rattus norvegicus
1.3.8.8 1.51
-
purified holoenzyme, palmitoleoyl-CoA Rattus norvegicus
1.3.8.8 1.52
-
purified apoenzyme, palmitoyl-CoA Rattus norvegicus
1.3.8.8 1.6
-
purified holoenzyme, stearoyl-CoA Rattus norvegicus
1.3.8.8 1.8
-
purified holoenzyme, dodecanoyl-CoA Rattus norvegicus
1.3.8.8 1.96
-
purified holoenzyme, palmitoyl-CoA Rattus norvegicus
1.3.8.8 2.1
-
purified holoenzyme, tetradecanoyl-CoA Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.3.8.1 -20°C, 10 mM KH2PO4, pH 8.0, 0.2 M NaCl, 50% glycerol, at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 butyryl-CoA + electron acceptor intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein Rattus norvegicus 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor terminal electron acceptor 2,6-dichlorophenol-indophenol Rattus norvegicus 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor intermediate electron acceptor phenazine methosulfate Rattus norvegicus 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
-
Rattus norvegicus 2-hexenoyl-CoA + reduced acceptor
-
?
1.3.8.1 pentanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-pentenoyl-CoA + reduced acceptor
-
?
1.3.8.7 acyl-CoA + acceptor electron acceptor: phenazine methosulfate Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
?
1.3.8.7 acyl-CoA + acceptor intermediary electron acceptor: 2,6-dichloroindophenol Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
?
1.3.8.7 acyl-CoA + acceptor final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
?
1.3.8.7 butanoyl-CoA + acceptor
-
Rattus norvegicus crotonyl-CoA + reduced acceptor
-
?
1.3.8.7 dodecanoyl-CoA + acceptor
-
Rattus norvegicus 2-dodecenoyl-CoA + reduced acceptor
-
?
1.3.8.7 n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol best substrate for medium-chain acyl-CoA dehydrogenase Rattus norvegicus 2-octenoyl-CoA + reduced acceptor
-
?
1.3.8.7 n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol no activity with tetradecanoyl-CoA and hexadecanoyl-CoA Rattus norvegicus 2-octenoyl-CoA + reduced acceptor
-
?
1.3.8.7 n-tetradecanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol best substrate for long-chain acyl-CoA dehydrogenase Rattus norvegicus 2-tetradecenoyl-CoA + reduced acceptor
-
?
1.3.8.7 pentanoyl-CoA + acceptor
-
Rattus norvegicus 2-pentenoyl-CoA + reduced acceptor
-
?
1.3.8.8 arachidonoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,5Z,8Z,11Z,14Z)-2,5,8,11,14-eicosapentaenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 decanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-decenoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.8 decanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E)-2-decenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 docosanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-docosenoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.8 docosanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-docosenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 dodecanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-dodecenoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.8 dodecanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E)-2-dodecenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 eicosanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus trans-2-eicosenoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.8 eicosanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus trans-2-eicosenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 erucoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,13Z)-2,13-docosadienoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 linolenoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,6Z,9Z,12Z)2,6,9,12-octadecatetraenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 linoleoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,9Z,12Z)-2,9,12-octadecatrienoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 octanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-octenoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.8 octanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-octenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 oleoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,9Z)-2,9-octadecadienoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 palmitoleoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,9Z)-2,9-hexadecadienoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 palmitoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.8 palmitoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-hexadecenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 stearoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-octadecenoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.8 stearoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-octadecenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 tetradecanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.8 tetradecanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-tetradecenoyl-CoA + reduced phenazine methosulfate
-
?

Subunits

EC Number Subunits Comment Organism
1.3.8.1 tetramer 4 * 41000, SDS-PAGE Rattus norvegicus
1.3.8.7 tetramer 4 * 45000, SDS-PAGE Rattus norvegicus
1.3.8.8 tetramer 4 * 38000-45200, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.7 6
-
tetradecanoyl-CoA long-chain acyl-CoA dehydrogenase Rattus norvegicus
1.3.8.7 31.2
-
hexanoyl-CoA medium-chain acyl-CoA dehydrogenase Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.8.7 5.5 8.7
-
Rattus norvegicus
1.3.8.8 5.5 8.7 stable for at least 10 min at 37°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.1 FAD 1 mol per mol of subunit Rattus norvegicus
1.3.8.7 FAD 1 mol per mol of subunit Rattus norvegicus
1.3.8.8 FAD
-
Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.8.1 0.0102
-
2-butenoyl-CoA
-
Rattus norvegicus