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Literature summary extracted from

  • Hines, V.; Keys, L.D.; Johnston, M.
    Purification and properties of the bovine liver mitochondrial dihydroorotate dehydrogenase [published erratum appears in J Biol Chem 1987 Nov 5;262(31):15322] (1986), J. Biol. Chem., 261, 11386-11392.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 (R)-dihydroorotate
-
 Bos taurus
1.3.5.2 5-Methylorotate
-
 Bos taurus
1.3.5.2 cis-5-methyldihydroorotate
-
 Bos taurus
1.3.5.2 Orotate competitive to dihydroorotate Bos taurus
1.3.5.2 Thenoyltrifluoroacetone
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.0011
-
 coenzyme Q6
-
 Bos taurus
1.3.5.2 0.0013
-
 coenzyme Q7 cosubstrate benzyl-S-dihydroorotate Bos taurus
1.3.5.2 0.0019
-
 methyl-S-dihydroorotate
-
 Bos taurus
1.3.5.2 0.0092
-
 S-dihydroorotate
-
 Bos taurus
1.3.5.2 0.184
-
 menadione
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 42000
-
 SDS-PAGE, glycerol gradient ultracentrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.2 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.2 103
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.3.5.2 4°C, pH 9.0, 50 mM Tris/HCl buffer, 0.1% Triton X-100, 3-4 weeks, t1/2: 2.5 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 (S)-dihydroorotate + coenzyme Q7
-
 Bos taurus orotate + reduced coenzyme Q7
-
 r
1.3.5.2 (S)-dihydroorotate + menadione
-
 Bos taurus orotate + menadiol
-
 r
1.3.5.2 (S)-dihydroorotate + ubiquinone
-
 Bos taurus orotate + ubiquinol
-
 ?
1.3.5.2 benzyl-(S)-dihydroorotate + acceptor
-
 Bos taurus benzyl orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor acceptor: quinones Bos taurus orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor acceptor: coenzyme Q6 Bos taurus orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor not acceptor: oxygen Bos taurus orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor acceptor coenzyme Q7 Bos taurus orotate + reduced acceptor
-
 ?
1.3.5.2 ethyl-dihydroorotate + acceptor
-
 Bos taurus ethyl-orotate + reduced acceptor
-
 ?
1.3.5.2 L-dihydroorotate + ubiquinone-6
-
 Bos taurus orotate + reduced ubiquinone-6
-
 ?
1.3.5.2 methyl-dihydroorotate + acceptor
-
 Bos taurus methyl-orotate + reduced acceptor
-
 ?
1.3.5.2 methyl-S-dihydroorotate + 2,6-dichlorophenolindophenol
-
 Bos taurus methylorotate + reduced 2,6-dichlorophenolindophenol
-
 r
1.3.5.2 additional information not substrate: 1-methyl-(S)-dihydroorotate, 3-methyl-dihydroorotate, 1,3-dimethyldihydroorotate Bos taurus ?
-
 ?
1.3.5.2 tert-butyl-dihydroorotate + acceptor
-
 Bos taurus tert-butyl-orotate + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.2 monomer 1 * 42000, SDS-PAGE, glycerol gradient centrifugation Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.5.2 4
-
 t1/2: 2.5 months Bos taurus
1.3.5.2 37
-
 3 h Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.2 8
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.5.2 6 10 no activity below pH 6.0 and above pH 10.0 Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.5.2 6
-
 unstable below Bos taurus
1.3.5.2 6.5 10.5 stable Bos taurus