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Literature summary extracted from

  • Miller, R.W.
    Dihydroorotate dehydrogenase (Neurospora) (1978), Methods Enzymol., 51, 63-69.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.3.5.2 dihydroorotate stabilizes during purification and dialysis and heat inactivation Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.2 fatty acids noncompetitive with respect to ubiquinone Neurospora crassa
1.3.5.2 Orotate
-
 Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.2 mitochondrion membrane Neurospora crassa 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.5.2 Iron 1.4 mol of ferric iron per 120000 g of active enzyme Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 210000
-
 gel filtration after phospholipase treatment Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.2 dihydroorotate + acceptor Neurospora crassa
-
 orotate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Neurospora crassa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.3.5.2 lipoprotein contains solvent-extractable fatty acids and phospholipids Neurospora crassa

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.2
-
 Neurospora crassa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.5.2 (S)-dihydroorotate + a quinone = orotate + a quinol ping-pong mechanism Neurospora crassa
a
1.3.5.2 (S)-dihydroorotate + a quinone = orotate + a quinol soluble quinones as second substrate Neurospora crassa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.5.2 mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.2 15
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.3.5.2 -20°C, several days Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 dihydroorotate + acceptor
-
 Neurospora crassa orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor acceptor: 2,6-dichlorophenolindophenol Neurospora crassa orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor acceptor: quinones Neurospora crassa orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor not acceptor: oxygen Neurospora crassa orotate + reduced acceptor
-
 ?
1.3.5.2 dihydroorotate + acceptor cytochrome b, cytochrome c, pyridine nucleotides (NAD+, NADP+) cannot serve as electron acceptors Neurospora crassa orotate + reduced acceptor
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.5.2 37
-
 assay at Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.2 FMN 1 mol of FMN per 120000 g of active enzyme Neurospora crassa