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Literature summary extracted from

  • Ikeda, Y.; Dabrowski, C.; Tanaka, K.
    Separation and properties of five distinct acyl-CoA dehydrogenases from rat liver mitochondria. Identification of a new 2-methyl branched chain acyl-CoA dehydrogenase (1983), J. Biol. Chem., 258, 1066-1076.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.1 0.27
-
phenazine methosulfate
-
Rattus norvegicus
1.3.8.4 0.00043
-
FAD
-
Rattus norvegicus
1.3.8.4 0.51
-
phenazine methosulfate
-
Rattus norvegicus
1.3.8.5 0.00051
-
FAD
-
Rattus norvegicus
1.3.8.5 0.48
-
phenazine methosulfate
-
Rattus norvegicus
1.3.8.7 0.0024
-
FAD
-
Rattus norvegicus
1.3.8.7 1.47
-
octanoyl-CoA with phenazine methosulfate Rattus norvegicus
1.3.8.8 0.0014
-
FAD
-
Rattus norvegicus
1.3.8.8 1.1
-
phenazine methosulfate
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.1 mitochondrion
-
Rattus norvegicus 5739
-
1.3.8.4 mitochondrion
-
Rattus norvegicus 5739
-
1.3.8.5 mitochondrion
-
Rattus norvegicus 5739
-
1.3.8.8 mitochondrion
-
Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.4 isovaleryl-CoA + acceptor Rattus norvegicus
-
3-methylcrotonyl-CoA + reduced acceptor
-
?
1.3.8.5 2-methylbutanoyl-CoA + electron transfer flavoprotein Rattus norvegicus
-
2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.1 Rattus norvegicus
-
-
-
1.3.8.4 Rattus norvegicus
-
-
-
1.3.8.5 Rattus norvegicus
-
-
-
1.3.8.7 Rattus norvegicus
-
-
-
1.3.8.8 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.4 using sonication, ammonium sulfate treatment, column chromatography on DEAE-Sephadex A-50, hydroxylapatite and isoelectrofocusing Rattus norvegicus
1.3.8.5
-
Rattus norvegicus
1.3.8.7
-
Rattus norvegicus
1.3.8.8 35-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.1 liver
-
Rattus norvegicus
-
1.3.8.4 liver
-
Rattus norvegicus
-
1.3.8.5 liver
-
Rattus norvegicus
-
1.3.8.7 liver
-
Rattus norvegicus
-
1.3.8.8 liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.1 butyryl-CoA + electron acceptor terminal electron acceptor 2,6-dichlorophenol-indophenol Rattus norvegicus 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 butyryl-CoA + electron acceptor intermediate electron acceptor phenazine methosulfate Rattus norvegicus 2-butenoyl-CoA + reduced acceptor
-
?
1.3.8.1 hexanoyl-CoA + phenazine methosulfate + dichloroindophenol 13% of butyryl-CoA activity Rattus norvegicus 2-hexenoyl-CoA + reduced acceptor
-
?
1.3.8.1 pentanoyl-CoA + phenazine methosulfate pentanoyl-CoA i.e. valeryl-CoA Rattus norvegicus 2-pentenoyl-CoA + reduced acceptor
-
?
1.3.8.4 isovaleryl-CoA + acceptor
-
Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
?
1.3.8.4 isovaleryl-CoA + acceptor isovaleryl-CoA is the preferred substrate Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
?
1.3.8.4 isovaleryl-CoA + acceptor acceptors: electron-transfer flavoprotein, phenazine methosulfate Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
?
1.3.8.4 isovaleryl-CoA + phenazine methosulfate
-
Rattus norvegicus 3-methylcrotonyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.4 n-valeryl-CoA + acceptor
-
Rattus norvegicus pent-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.5 (S)-2-methylbutanoyl-CoA + acceptor acceptors: electron transfer flavoprotein, phenazine methosulfate, meldola's blue Rattus norvegicus 2-methylbut-2-enoyl-CoA + reduced acceptor
-
r
1.3.8.5 2-methylbutanoyl-CoA + electron transfer flavoprotein
-
Rattus norvegicus 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
-
r
1.3.8.5 isobutyryl-CoA + acceptor
-
Rattus norvegicus methacrylyl-CoA + reduced acceptor
-
?
1.3.8.5 isovaleryl-CoA + phenazine methosulfate
-
Rattus norvegicus ?
-
r
1.3.8.7 acyl-CoA + acceptor electron acceptor: phenazine methosulfate Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
?
1.3.8.7 acyl-CoA + acceptor specific for chain lengths C6-C12 Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
?
1.3.8.7 acyl-CoA + acceptor intermediary electron acceptor: 2,6-dichloroindophenol Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
?
1.3.8.7 acyl-CoA + acceptor final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
?
1.3.8.7 decanoyl-CoA + acceptor 72% of activity with hexanoyl-CoA Rattus norvegicus 2-decenoyl-CoA + reduced acceptor
-
?
1.3.8.7 decanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
-
Rattus norvegicus 2-trans-decenoyl-CoA + reduced acceptor
-
?
1.3.8.7 heptadecan-2-onyl-dethio-CoA + phenazine methosulfate + 2,6-dichloroindophenol
-
Rattus norvegicus ?
-
?
1.3.8.7 hexadecanoyl-CoA + acceptor 55% of activity with hexanoyl-CoA Rattus norvegicus 2-hexadecenoyl-CoA + reduced acceptor
-
?
1.3.8.7 hexanoyl-CoA + acceptor best substrate Rattus norvegicus 2-hexenoyl-CoA + reduced acceptor
-
?
1.3.8.7 hexanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
-
Rattus norvegicus 2-trans-hexenoyl-CoA + reduced acceptor
-
?
1.3.8.7 lauroyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
-
Rattus norvegicus 2-trans-dodecenoyl-CoA + reduced acceptor
-
?
1.3.8.7 n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol 82% of activity with hexanoyl-CoA Rattus norvegicus 2-octenoyl-CoA + reduced acceptor
-
?
1.3.8.7 n-tetradecanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol best substrate for long-chain acyl-CoA dehydrogenase Rattus norvegicus 2-tetradecenoyl-CoA + reduced acceptor
-
?
1.3.8.7 pentanoyl-CoA + acceptor 68% of activity with hexanoyl-CoA Rattus norvegicus 2-pentenoyl-CoA + reduced acceptor
-
?
1.3.8.8 decanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E)-2-decenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 dodecanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E)-2-dodecenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 eicosanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus trans-2-eicosenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 octanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-octenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 palmitoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-hexadecenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 stearoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-octadecenoyl-CoA + reduced phenazine methosulfate
-
?
1.3.8.8 tetradecanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-tetradecenoyl-CoA + reduced phenazine methosulfate
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.4 FAD
-
Rattus norvegicus
1.3.8.5 FAD
-
Rattus norvegicus
1.3.8.7 FAD FAD is readily lost upon purification Rattus norvegicus
1.3.8.8 FAD
-
Rattus norvegicus