BRENDA - Enzyme Database

Purification and properties of short chain acyl-CoA, medium chain acyl-CoA, and isovaleryl-CoA dehydrogenases from human liver

Finocchiaro, G.; Ito, M.; Tanaka, K.; J. Biol. Chem. 262, 7982-7989 (1987) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.8.1
(methylenecyclopropyl)acetyl-CoA
0.01 mM, 81% inhibition, 0.001 mM, 49% inhibition
Homo sapiens
1.3.8.1
iodoacetamide
2 mM, 63% inhibition
Homo sapiens
1.3.8.1
N-ethylmaleimide
2 mM, 41% inhibition, 0.2 mM, 23% inhibition
Homo sapiens
1.3.8.4
(methylenecyclopropyl)acetyl-CoA
at 0.001 mM 21% activity remains; at 0.01 mM 3% activity remains
Homo sapiens
1.3.8.4
iodoacetamide
at 2 mM 19% activity remains
Homo sapiens
1.3.8.4
Methylmercury iodide
-
Homo sapiens
1.3.8.4
N-ethylmaleimide
at 2 mM 64% activity remains
Homo sapiens
1.3.8.4
p-chloromercuribenzoate
at 0.1 mM 18% activity remains
Homo sapiens
1.3.8.4
p-hydroxymercuribenzoate
-
Homo sapiens
1.3.8.7
(methylenecyclopropyl)acetyl-CoA
0.01 mM, 95% inhibition
Homo sapiens
1.3.8.7
(R)-(-)-(methylenecyclopropyl)acetyl-CoA
-
Homo sapiens
1.3.8.7
iodoacetamide
2 mM, 73% inhibition
Homo sapiens
1.3.8.7
N-ethylmaleimide
2 mM, 43% inhibition
Homo sapiens
1.3.8.7
p-chloromercuribenzoate
0.1 mM, 17% inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.8.1
0.0041
-
electron transfer flavoprotein
-
Homo sapiens
1.3.8.1
0.0129
-
butyryl-CoA
-
Homo sapiens
1.3.8.1
0.0333
-
pentanoyl-CoA
-
Homo sapiens
1.3.8.1
0.0339
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.1
0.179
-
phenazine methosulfate
-
Homo sapiens
1.3.8.4
0.0038
-
electron-transfer flavoprotein
-
Homo sapiens
1.3.8.4
0.014
-
isovaleryl-CoA
-
Homo sapiens
1.3.8.4
0.0167
-
valeryl-CoA
-
Homo sapiens
1.3.8.4
0.0167
0.4
n-valeryl-CoA
-
Homo sapiens
1.3.8.4
0.02
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.4
0.025
-
butyryl-CoA
-
Homo sapiens
1.3.8.4
0.833
-
phenazine methosulfate
-
Homo sapiens
1.3.8.7
0.0034
-
electron transfer flavoprotein
-
Homo sapiens
1.3.8.7
0.00714
-
butyryl-CoA
-
Homo sapiens
1.3.8.7
0.008
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.7
0.0091
-
decanoyl-CoA
-
Homo sapiens
1.3.8.7
0.01
-
tetradecenoyl-CoA
-
Homo sapiens
1.3.8.7
0.0216
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.7
0.023
-
palmitoyl-CoA
-
Homo sapiens
1.3.8.7
0.0563
-
valeryl-CoA
-
Homo sapiens
1.3.8.7
1.67
-
phenazine methosulfate
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.8.4
mitochondrion
-
Homo sapiens
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.8.1
41200
-
4 * 41200, SDS-PAGE
Homo sapiens
1.3.8.1
168000
-
gel filtration
Homo sapiens
1.3.8.4
42000
-
4 * 42000, SDS-PAGE
Homo sapiens
1.3.8.4
172000
-
gel filtration
Homo sapiens
1.3.8.7
44000
-
4 * 44000, SDS-PAGE
Homo sapiens
1.3.8.7
178000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.3.8.4
isovaleryl-CoA + acceptor
Homo sapiens
-
3-methylcrotonyl-CoA + reduced acceptor
-
Homo sapiens
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.3.8.1
Homo sapiens
-
-
-
1.3.8.4
Homo sapiens
-
-
-
1.3.8.7
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.3.8.1
-
Homo sapiens
1.3.8.4
using ammonium sulfate fractionation followed by DEAE-Sephadex A-50, hydroxyapatite, Matrex Gel Blue A, agarose-hexane-CoA, and Bio-Gel A-0.5 column chromatography
Homo sapiens
1.3.8.7
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.8.1
liver
-
Homo sapiens
-
1.3.8.4
liver
-
Homo sapiens
-
1.3.8.7
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.8.1
7.4
-
-
Homo sapiens
1.3.8.4
0.01
-
propionyl-CoA as substrate and phenazine methosulfate as acceptor or hexanoyl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
0.03
-
butyryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
0.09
-
valeryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
0.17
-
S-2-methylbutyryl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.4
0.51
-
butyryl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.4
0.53
-
isovaleryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
0.64
-
hexanoyl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.4
0.72
-
isovaleryl-CoA as substrate and 0.012 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
1.09
-
valeryl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.4
2.91
-
isovaleryl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.7
6.75
-
-
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein
391201
Homo sapiens
2-butenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
butyryl-CoA + electron acceptor
terminal electron acceptor 2,6-dichlorophenol-indophenol
391201
Homo sapiens
2-butenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptor phenazine methosulfate
391201
Homo sapiens
2-butenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
hexanoyl-CoA + electron transfer flavoprotein
-
391201
Homo sapiens
2-hexenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
-
391201
Homo sapiens
2-hexenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
pentanoyl-CoA + electron transfer flavoprotein
-
391201
Homo sapiens
2-pentenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
pentanoyl-CoA + phenazine methosulfate
pentanoyl-CoA i.e. valeryl-CoA
391201
Homo sapiens
2-pentenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
propionyl-CoA + electron transfer flavoprotein
-
391201
Homo sapiens
2-propenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
propionyl-CoA + phenazine methosulfate
-
391201
Homo sapiens
2-propenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
butyryl-CoA + acceptor
acceptor: phenazine methosulfate
391201
Homo sapiens
but-2-enoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
hexanoyl-CoA + acceptor
acceptor: phenazine methosulfate
391201
Homo sapiens
hex-2-enoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
isovaleryl-CoA + acceptor
-
391201
Homo sapiens
3-methylcrotonyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
isovaleryl-CoA + acceptor
acceptors: electron-transfer flavoprotein, phenazine methosulfate
391201
Homo sapiens
3-methylcrotonyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
isovaleryl-CoA + electron-transfer flavoprotein
-
391201
Homo sapiens
3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
-
-
r
1.3.8.4
isovaleryl-CoA + phenazine methosulfate
-
391201
Homo sapiens
3-methylcrotonyl-CoA + reduced phenazine methosulfate
-
-
-
?
1.3.8.7
acyl-CoA + acceptor
electron acceptor: phenazine methosulfate
391201
Homo sapiens
2,3-dehydroacyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
acyl-CoA + acceptor
chain-length specificity depending on electron acceptor: with phenazine methosulfate hexanoyl-CoA, with electron transfer flavoprotein, octanoyl-CoA
391201
Homo sapiens
2,3-dehydroacyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
acyl-CoA + acceptor
intermediary electron acceptor: 2,6-dichloroindophenol
391201
Homo sapiens
2,3-dehydroacyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
acyl-CoA + acceptor
final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue
391201
Homo sapiens
2,3-dehydroacyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
butanoyl-CoA + acceptor
-
391201
Homo sapiens
crotonyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
decanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein
391201
Homo sapiens
2-decenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
dodecanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein
391201
Homo sapiens
2-dodecenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
hexadecanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein, very low activity
391201
Homo sapiens
2-hexadecenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
hexanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein, best substrate with phenazine methosulfate
391201
Homo sapiens
2-hexenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
best substrate with electron transferring flavoprotein
391201
Homo sapiens
2-octenoyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
octadecanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein, extremely low activity
391201
Homo sapiens
2-octadecenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
pentanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein
391201
Homo sapiens
2-pentenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
tetradecanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein, low activity
391201
Homo sapiens
2-tetradecenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
tetradecenoyl-CoA + reduced electron transfer flavoprotein
-
391201
Homo sapiens
tetradecanoyl-CoA + electron transfer flavoprotein
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.3.8.1
tetramer
4 * 41200, SDS-PAGE
Homo sapiens
1.3.8.4
tetramer
4 * 42000, SDS-PAGE
Homo sapiens
1.3.8.7
tetramer
4 * 44000, SDS-PAGE
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.8.1
16.5
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.1
20.8
-
butyryl-CoA
-
Homo sapiens
1.3.8.1
40.7
-
valeryl-CoA
-
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.8.1
8
-
-
Homo sapiens
1.3.8.4
8
-
isovaleryl-CoA + phenazine methosulfate
Homo sapiens
1.3.8.7
8
-
-
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.8.1
6.5
9
less than 50% of maximal activity above and below
Homo sapiens
1.3.8.7
6
9.5
-
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.8.1
FAD
1 mol per mol of subunit
Homo sapiens
1.3.8.4
FAD
1 mol of FAD per subunit
Homo sapiens
1.3.8.7
FAD
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.8.1
FAD
1 mol per mol of subunit
Homo sapiens
1.3.8.4
FAD
1 mol of FAD per subunit
Homo sapiens
1.3.8.7
FAD
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.8.1
(methylenecyclopropyl)acetyl-CoA
0.01 mM, 81% inhibition, 0.001 mM, 49% inhibition
Homo sapiens
1.3.8.1
iodoacetamide
2 mM, 63% inhibition
Homo sapiens
1.3.8.1
N-ethylmaleimide
2 mM, 41% inhibition, 0.2 mM, 23% inhibition
Homo sapiens
1.3.8.4
(methylenecyclopropyl)acetyl-CoA
at 0.01 mM 3% activity remains
Homo sapiens
1.3.8.4
(methylenecyclopropyl)acetyl-CoA
at 0.001 mM 21% activity remains
Homo sapiens
1.3.8.4
iodoacetamide
at 2 mM 19% activity remains
Homo sapiens
1.3.8.4
Methylmercury iodide
-
Homo sapiens
1.3.8.4
N-ethylmaleimide
at 2 mM 64% activity remains
Homo sapiens
1.3.8.4
p-chloromercuribenzoate
at 0.1 mM 18% activity remains
Homo sapiens
1.3.8.4
p-hydroxymercuribenzoate
-
Homo sapiens
1.3.8.7
(methylenecyclopropyl)acetyl-CoA
0.01 mM, 95% inhibition
Homo sapiens
1.3.8.7
(R)-(-)-(methylenecyclopropyl)acetyl-CoA
-
Homo sapiens
1.3.8.7
iodoacetamide
2 mM, 73% inhibition
Homo sapiens
1.3.8.7
N-ethylmaleimide
2 mM, 43% inhibition
Homo sapiens
1.3.8.7
p-chloromercuribenzoate
0.1 mM, 17% inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.8.1
0.0041
-
electron transfer flavoprotein
-
Homo sapiens
1.3.8.1
0.0129
-
butyryl-CoA
-
Homo sapiens
1.3.8.1
0.0333
-
pentanoyl-CoA
-
Homo sapiens
1.3.8.1
0.0339
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.1
0.179
-
phenazine methosulfate
-
Homo sapiens
1.3.8.4
0.0038
-
electron-transfer flavoprotein
-
Homo sapiens
1.3.8.4
0.014
-
isovaleryl-CoA
-
Homo sapiens
1.3.8.4
0.0167
-
valeryl-CoA
-
Homo sapiens
1.3.8.4
0.0167
0.4
n-valeryl-CoA
-
Homo sapiens
1.3.8.4
0.02
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.4
0.025
-
butyryl-CoA
-
Homo sapiens
1.3.8.4
0.833
-
phenazine methosulfate
-
Homo sapiens
1.3.8.7
0.0034
-
electron transfer flavoprotein
-
Homo sapiens
1.3.8.7
0.00714
-
butyryl-CoA
-
Homo sapiens
1.3.8.7
0.008
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.7
0.0091
-
decanoyl-CoA
-
Homo sapiens
1.3.8.7
0.01
-
tetradecenoyl-CoA
-
Homo sapiens
1.3.8.7
0.0216
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.7
0.023
-
palmitoyl-CoA
-
Homo sapiens
1.3.8.7
0.0563
-
valeryl-CoA
-
Homo sapiens
1.3.8.7
1.67
-
phenazine methosulfate
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.8.4
mitochondrion
-
Homo sapiens
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.8.1
41200
-
4 * 41200, SDS-PAGE
Homo sapiens
1.3.8.1
168000
-
gel filtration
Homo sapiens
1.3.8.4
42000
-
4 * 42000, SDS-PAGE
Homo sapiens
1.3.8.4
172000
-
gel filtration
Homo sapiens
1.3.8.7
44000
-
4 * 44000, SDS-PAGE
Homo sapiens
1.3.8.7
178000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.3.8.4
isovaleryl-CoA + acceptor
Homo sapiens
-
3-methylcrotonyl-CoA + reduced acceptor
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.8.1
-
Homo sapiens
1.3.8.4
using ammonium sulfate fractionation followed by DEAE-Sephadex A-50, hydroxyapatite, Matrex Gel Blue A, agarose-hexane-CoA, and Bio-Gel A-0.5 column chromatography
Homo sapiens
1.3.8.7
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.8.1
liver
-
Homo sapiens
-
1.3.8.4
liver
-
Homo sapiens
-
1.3.8.7
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.8.1
7.4
-
-
Homo sapiens
1.3.8.4
0.01
-
propionyl-CoA as substrate and phenazine methosulfate as acceptor or hexanoyl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
0.03
-
butyryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
0.09
-
valeryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
0.17
-
S-2-methylbutyryl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.4
0.51
-
butyryl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.4
0.53
-
isovaleryl-CoA as substrate and 0.0056 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
0.64
-
hexanoyl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.4
0.72
-
isovaleryl-CoA as substrate and 0.012 mM electron transfer flavoprotein as acceptor
Homo sapiens
1.3.8.4
1.09
-
valeryl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.4
2.91
-
isovaleryl-CoA as substrate and phenazine methosulfate as acceptor
Homo sapiens
1.3.8.7
6.75
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein
391201
Homo sapiens
2-butenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
butyryl-CoA + electron acceptor
terminal electron acceptor 2,6-dichlorophenol-indophenol
391201
Homo sapiens
2-butenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
butyryl-CoA + electron acceptor
intermediate electron acceptor phenazine methosulfate
391201
Homo sapiens
2-butenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
hexanoyl-CoA + electron transfer flavoprotein
-
391201
Homo sapiens
2-hexenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol
-
391201
Homo sapiens
2-hexenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
pentanoyl-CoA + electron transfer flavoprotein
-
391201
Homo sapiens
2-pentenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
pentanoyl-CoA + phenazine methosulfate
pentanoyl-CoA i.e. valeryl-CoA
391201
Homo sapiens
2-pentenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
propionyl-CoA + electron transfer flavoprotein
-
391201
Homo sapiens
2-propenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.1
propionyl-CoA + phenazine methosulfate
-
391201
Homo sapiens
2-propenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
butyryl-CoA + acceptor
acceptor: phenazine methosulfate
391201
Homo sapiens
but-2-enoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
hexanoyl-CoA + acceptor
acceptor: phenazine methosulfate
391201
Homo sapiens
hex-2-enoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
isovaleryl-CoA + acceptor
-
391201
Homo sapiens
3-methylcrotonyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
isovaleryl-CoA + acceptor
acceptors: electron-transfer flavoprotein, phenazine methosulfate
391201
Homo sapiens
3-methylcrotonyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.4
isovaleryl-CoA + electron-transfer flavoprotein
-
391201
Homo sapiens
3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
-
-
r
1.3.8.4
isovaleryl-CoA + phenazine methosulfate
-
391201
Homo sapiens
3-methylcrotonyl-CoA + reduced phenazine methosulfate
-
-
-
?
1.3.8.7
acyl-CoA + acceptor
electron acceptor: phenazine methosulfate
391201
Homo sapiens
2,3-dehydroacyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
acyl-CoA + acceptor
chain-length specificity depending on electron acceptor: with phenazine methosulfate hexanoyl-CoA, with electron transfer flavoprotein, octanoyl-CoA
391201
Homo sapiens
2,3-dehydroacyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
acyl-CoA + acceptor
intermediary electron acceptor: 2,6-dichloroindophenol
391201
Homo sapiens
2,3-dehydroacyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
acyl-CoA + acceptor
final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue
391201
Homo sapiens
2,3-dehydroacyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
butanoyl-CoA + acceptor
-
391201
Homo sapiens
crotonyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
decanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein
391201
Homo sapiens
2-decenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
dodecanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein
391201
Homo sapiens
2-dodecenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
hexadecanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein, very low activity
391201
Homo sapiens
2-hexadecenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
hexanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein, best substrate with phenazine methosulfate
391201
Homo sapiens
2-hexenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol
best substrate with electron transferring flavoprotein
391201
Homo sapiens
2-octenoyl-CoA + reduced acceptor
-
-
-
?
1.3.8.7
octadecanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein, extremely low activity
391201
Homo sapiens
2-octadecenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
pentanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein
391201
Homo sapiens
2-pentenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
tetradecanoyl-CoA + acceptor
acceptors: phenazine methosulfate or electron transferring flavoprotein, low activity
391201
Homo sapiens
2-tetradecenoyl-CoA + reduced acceptor
-
391201
Homo sapiens
?
1.3.8.7
tetradecenoyl-CoA + reduced electron transfer flavoprotein
-
391201
Homo sapiens
tetradecanoyl-CoA + electron transfer flavoprotein
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.8.1
tetramer
4 * 41200, SDS-PAGE
Homo sapiens
1.3.8.4
tetramer
4 * 42000, SDS-PAGE
Homo sapiens
1.3.8.7
tetramer
4 * 44000, SDS-PAGE
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.3.8.1
16.5
-
hexanoyl-CoA
-
Homo sapiens
1.3.8.1
20.8
-
butyryl-CoA
-
Homo sapiens
1.3.8.1
40.7
-
valeryl-CoA
-
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.8.1
8
-
-
Homo sapiens
1.3.8.4
8
-
isovaleryl-CoA + phenazine methosulfate
Homo sapiens
1.3.8.7
8
-
-
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.8.1
6.5
9
less than 50% of maximal activity above and below
Homo sapiens
1.3.8.7
6
9.5
-
Homo sapiens