EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.4 | (methylenecyclopropyl)acetyl-CoA | completely inhibits at 0.01 mM | Rattus norvegicus | |
1.3.8.4 | 3-methylcrotonyl-CoA | - |
Rattus norvegicus | |
1.3.8.4 | Ag+ | completely inhibits at 0.01 mM | Rattus norvegicus | |
1.3.8.4 | Cu2+ | completely inhibits at 0.01 mM | Rattus norvegicus | |
1.3.8.4 | Hg2+ | completely inhibits at 0.1 mM | Rattus norvegicus | |
1.3.8.4 | Methylmercury iodide | completely inhibits at a concentration of 0.1 mM | Rattus norvegicus | |
1.3.8.4 | N-ethylmaleimide | completely inhibits at a concentration of 2 mM | Rattus norvegicus | |
1.3.8.4 | p-hydroxymercuribenzoate | completely inhibits at a concentration of 0.01 mM | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.4 | 0.0167 | 0.4 | n-valeryl-CoA | - |
Rattus norvegicus | |
1.3.8.4 | 0.033 | - |
isovaleryl-CoA | with phenazine methosulfate as an artificial electron acceptor | Rattus norvegicus | |
1.3.8.4 | 0.4 | - |
n-valeryl-CoA | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.8.4 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.8.4 | 43000 | - |
4 * 43000, SDS-PAGE | Rattus norvegicus |
1.3.8.4 | 175000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.4 | isovaleryl-CoA + acceptor | Rattus norvegicus | electron acceptor: electron-transfer flavoprotein serves as a natural electron acceptor for the enzyme with isovaleryl-CoA as substrate | 3-methylcrotonyl-CoA + reduced acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.4 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.4 | using sonication, ammonium sulfate treatment and column chromatography on DEAE-Sephadex A-50, hydroxylapatite, Matrex Gel Blue A and BioGel A-0.5 m | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.8.4 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.8.4 | 0.086 | - |
substrate: n-butyryl-CoA | Rattus norvegicus |
1.3.8.4 | 0.832 | - |
substrate: n-valeryl-CoA | Rattus norvegicus |
1.3.8.4 | 2.68 | - |
substrate: isovaleryl-CoA | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.4 | isovaleryl-CoA + acceptor | isovaleryl-CoA is the preferred substrate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | isovaleryl-CoA + acceptor | acceptors: electron-transfer flavoprotein, phenazine methosulfate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | isovaleryl-CoA + acceptor | electron acceptor: electron-transfer flavoprotein serves as a natural electron acceptor for the enzyme with isovaleryl-CoA as substrate | Rattus norvegicus | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | n-valeryl-CoA + acceptor | - |
Rattus norvegicus | pent-2-enoyl-CoA + reduced acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.4 | tetramer | 4 * 43000, SDS-PAGE | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.8.4 | 8 | - |
isovaleryl-CoA + phenazine methosulfate | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.4 | FAD | the enzyme contains FAD as a prosthetic group, 0.6 mol FAD per mol of subunit | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.4 | 0.02 | - |
3-methylcrotonyl-CoA | - |
Rattus norvegicus |