EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.1 | 2-Thenoyltrifluoroacetone | 50% inhibition at 3 mM | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.5.1 | 13000 | - |
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE | Escherichia coli |
1.3.5.1 | 28000 | - |
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE | Escherichia coli |
1.3.5.1 | 64000 | - |
1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.1 | Escherichia coli | - |
succinate dehydrogenase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.5.1 | using solubilization with polyoxyethylene-9-lauryl ether and column chromatography on DEAE-Sepharose CL-6B | Escherichia coli |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.3.5.1 | the isolated enzyme is resolved into a reconstitutively active, two-subunit succinate dehydrogenase and a two-subunit membrane anchoring protein fraction by alkaline treatment in the presence of urea followed by DEAE-Sepharose CL-6B column chromatography, maximum reconstitution is obtained when the weight ratio of succinate dehydrogenase to the two-subunit membrane anchoring protein reaches 5.26 | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.5.1 | 16 | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.1 | succinate + ubiquinone | - |
Escherichia coli | fumarate + ubiquinol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.5.1 | tetramer | 1 * 64000 + 1 * 28000 + 1+ 14000 + 1 * 13000, SDS-PAGE | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.1 | cytochrome b | the isolated two-subunit membrane anchoring protein contains 35 nmol cytochrome b-556 per mg protein | Escherichia coli |