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Literature summary extracted from

  • Weiner, J.H.; Cammack, R.; Cole, S.T.; Condon, C.; Honore, N.; Lemire, B.D.; Shaw, G.
    A mutant of Escherichia coli fumarate reductase decoupled from electron transport (1986), Proc. Natl. Acad. Sci. USA, 83, 2056-2060.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.1 cloning of a mutant fumarate reductase operon Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.1 H82R menaquinone, ubiquinone and b-type cytochrome levels are present in normal amounts, the mutation alters the electron transfer properties of the iron-sulfur and flavin redox centers of the catalytic domain, functional electron flow from 2,3-dimethyl-1,4-naphthoquinone or from the electron transport chain is impaired, the mutant can be reduced normally by single-electron donors such as benzyl viologen Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 2-(n-heptyl)-4-hydroxy-quinoline N-oxide
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.1 13000
-
 fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase Escherichia coli
1.3.5.1 15000
-
 fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase Escherichia coli
1.3.5.1 27000
-
 fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase Escherichia coli
1.3.5.1 69000
-
 fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Escherichia coli
-
 fumarate reductase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 fumarate + 2,3-dimethyl-1,4-naphthohydroquinone mutation of His-82 to Arg in fumarate reductase subunit C prevents oxidation of 2,3-dimethyl-1,4-naphthohydroquinone Escherichia coli succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.5.1 tetramer fumarate reductase, membrane-extrinsic domain: 1 * 69000 + 1 * 27000, membrane-intrinsic domain: 1 * 15000 + 1 * 13000 containing cytochrome b, necessary for converting succinate dehydrogenase EC 1.3.99.1 into succinate-ubiquinone oxidoreductase Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 additional information the enzyme contains iron-sulfur centers Escherichia coli