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Literature summary extracted from
- Isolation and functional aspects of the fumarate reductase involved in the phosphorylative electron transport of Vibrio succinogenes (1980), Biochim. Biophys. Acta, 591, 275-288.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.5.1 | fumarate | - |
Wolinella succinogenes |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.5.1 | 0.12 | - |
2,3-dimethyl-1,4-naphthohydroquinone | - |
Wolinella succinogenes | |
| 1.3.5.1 | 0.35 | - |
fumarate | donor menadiol | Wolinella succinogenes | |
| 1.3.5.1 | 20 | - |
succinate | - |
Wolinella succinogenes | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.5.1 | cell membrane | - |
Wolinella succinogenes | - |
- |
| 1.3.5.1 | cytoplasmic membrane | - |
Wolinella succinogenes | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.5.1 | Fe | - |
Wolinella succinogenes | |
| 1.3.5.1 | Iron | - |
Wolinella succinogenes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.5.1 | 25000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
| 1.3.5.1 | 25000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
| 1.3.5.1 | 31000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
| 1.3.5.1 | 31000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
| 1.3.5.1 | 31000 | - |
1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
| 1.3.5.1 | 79000 | - |
1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
| 1.3.5.1 | 79000 | - |
enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
| 1.3.5.1 | 79000 | - |
1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
| 1.3.5.1 | 110000 | 114000 | excluding cytochrome b, calculation from FAD-content and subunit composition | Wolinella succinogenes |
| 1.3.5.1 | 147000 | - |
calculation from Stokes radius and sedimentation coefficient | Wolinella succinogenes |
| 1.3.5.1 | 160000 | 167000 | including cytochrome b, calculation from FAD-content and subunit composition | Wolinella succinogenes |
| 1.3.5.1 | 200000 | - |
including cytochrome b, sedimentation equilibrium centrifugation | Wolinella succinogenes |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.5.1 | Wolinella succinogenes | - |
formerly Vibrio succinogenes, fumarate reductase | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.5.1 | - |
Wolinella succinogenes |
| 1.3.5.1 | using solubilization with Triton X-100, chromatography on hydroxyapatite column and DEAE-Sephadex column, the enzyme elutes from the ion-exchange column in two forms, one containing and the other lacking cytochrome b | Wolinella succinogenes |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.5.1 | 140 | - |
- |
Wolinella succinogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.5.1 | fumarate + 2,3-dimethyl-1,4-naphthohydroquinone | cytochrome b-dependent in both directions | Wolinella succinogenes | succinate + 2,3-dimethyl-1,4-naphthoquinone | - |
r | |
| 1.3.5.1 | fumarate + anthrahydroquinonesulfonate | reaction catalyzed in the presence or absence of cytochrome b | Wolinella succinogenes | succinate + anthraquinonesulfonate | - |
? | |
| 1.3.5.1 | fumarate + electron donor | donor: anthrahydroquinonesulfonate | Wolinella succinogenes | succinate + oxidized donor | - |
r | |
| 1.3.5.1 | succinate + electron acceptor | acceptor: methylene blue (oxidized) | Wolinella succinogenes | fumarate + reduced acceptor | - |
r | |
| 1.3.5.1 | succinate + electron acceptor | acceptor: ferricyanide | Wolinella succinogenes | fumarate + reduced acceptor | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.5.1 | dimer | 1 * 79000 + 1 * 31000, SDS-PAGE | Wolinella succinogenes |
| 1.3.5.1 | tetramer | 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE | Wolinella succinogenes |
| 1.3.5.1 | trimer | enzyme containing cytochrome b, 1 * 79000 + 1 * 31000 + 1 * 25000 | Wolinella succinogenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.5.1 | cytochrome b | 2 mol per mol FAD | Wolinella succinogenes | |
| 1.3.5.1 | cytochrome b | 2 mol per mol of FAD | Wolinella succinogenes | |
| 1.3.5.1 | FAD | - |
Wolinella succinogenes | |
| 1.3.5.1 | FAD | one protein-bound FAD linked to the 79000 Da peptide | Wolinella succinogenes | |
| 1.3.5.1 | additional information | the enzyme contains iron-sulfur centers | Wolinella succinogenes |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.5.1 | 0.35 | - |
fumarate | - |
Wolinella succinogenes | |
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