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Literature summary extracted from

  • Unden, G.; Kr๖ger, A.
    The function of the subunits of the fumarate reductase complex of Vibrio succinogenes (1981), Eur. J. Biochem., 120, 577-584.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.1 25000
-
 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE Wolinella succinogenes
1.3.5.1 31000
-
 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE Wolinella succinogenes
1.3.5.1 79000
-
 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE Wolinella succinogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.1 fumarate + 2,3-dimethyl-1,4-naphthohydroquinone Wolinella succinogenes
-
 succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 ?
1.3.5.1 additional information Wolinella succinogenes the fumarate reductase complex has two different reactive sites, which are essential for its function in the phosphorylative electron transport of the bacterium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Wolinella succinogenes
-
 formerly Vibrio succinogenes, fumarate reductase
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.3.5.1 the cleavage of the complex causes the complete loss of activity of fumarate reduction by dimethylnaphthohydroquinone, the activity can be restored by coprecipitation of the three subunits of MW: 79000 Da, 31000 Da and 25000 Da Wolinella succinogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 fumarate + 2,3-dimethyl-1,4-naphthohydroquinone
-
 Wolinella succinogenes succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 ?
1.3.5.1 fumarate + 2,3-dimethyl-1,4-naphthohydroquinone reaction catalyzed by fumarate reductase complex, the site of the complex reacting with fumarate is situated on the 79000 Da subunit, and the site reacting with dimethylnaphthohydroquinone is cytochrome b Wolinella succinogenes succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 ?
1.3.5.1 fumarate + 2,3-dimethyl-1,4-naphthohydroquinone reaction catalyzed by fumarate reductase complex, the site of the complex reacting with fumarate is situated on the 79000 Da subunit, and the site reacting with dimethylnaphthohydroquinone is cytochrome b Wolinella succinogenes succinate + 2,3-dimethyl-1,4-naphthoquinone
-
 r
1.3.5.1 additional information the fumarate reductase complex has two different reactive sites, which are essential for its function in the phosphorylative electron transport of the bacterium Wolinella succinogenes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.1 tetramer 1 * 79000, FAD-binding subunit, 1 * 31000, Fe-S cluster containing subunit, 2 * 25000, cytochrome b containing subunits, SDS-PAGE Wolinella succinogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.1 20
-
 fumarate based on the content of the MW 79000 peptide, reduction of fumarate with dimethylnaphthohydroquinone Wolinella succinogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 cytochrome b has a MW of 25000 Da, a midpoint potential of - 15 mV and is reducible by dimethylnaphthohydroquinone in the absence of the other subunits Wolinella succinogenes
1.3.5.1 additional information measurement of the redox potentials of the sulfur-centers Wolinella succinogenes