EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.3.6 | MnCl2 | slightly activating | Glutamicibacter nicotianae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.3.6 | AgNO3 | - |
Glutamicibacter nicotianae | |
1.3.3.6 | HgCl2 | - |
Glutamicibacter nicotianae | |
1.3.3.6 | p-chloromercuribenzoate | slight inhibition | Glutamicibacter nicotianae | |
1.3.3.6 | Phenylmethylsulfonylfluoride | slight inhibition | Glutamicibacter nicotianae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.3.6 | 0.061 | - |
octanoyl-CoA | - |
Glutamicibacter nicotianae | |
1.3.3.6 | 0.092 | - |
hexanoyl-CoA | - |
Glutamicibacter nicotianae | |
1.3.3.6 | 0.1319 | - |
butyryl-CoA | - |
Glutamicibacter nicotianae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.3.6 | 50000 | - |
2 * 50000, SDS-PAGE | Glutamicibacter nicotianae |
1.3.3.6 | 100000 | - |
gel filtration | Glutamicibacter nicotianae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.3.6 | Glutamicibacter nicotianae | - |
inducible | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.3.6 | - |
Glutamicibacter nicotianae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.3.6 | 77.14 | - |
purified enzyme | Glutamicibacter nicotianae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.6 | acyl-CoA + O2 | highly specific for short-chain fatty acids | Glutamicibacter nicotianae | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
1.3.3.6 | butyryl-CoA + O2 | - |
Glutamicibacter nicotianae | trans-2-butenoyl-CoA + H2O2 | - |
? | |
1.3.3.6 | hexanoyl-CoA + O2 | - |
Glutamicibacter nicotianae | (2E)-hex-2-enoyl-CoA + H2O2 | - |
? | |
1.3.3.6 | octanoyl-CoA + O2 | - |
Glutamicibacter nicotianae | trans-2-octenoyl-CoA + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.3.6 | dimer | 2 * 50000, SDS-PAGE | Glutamicibacter nicotianae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.3.6 | additional information | - |
FAD enhances thermal stability | Glutamicibacter nicotianae |
1.3.3.6 | 60 | - |
30 min, with butyryl-CoA and FAD, 50% loss of activity | Glutamicibacter nicotianae |
1.3.3.6 | 60 | - |
30 min, with butyryl-CoA, without FAD, 85% loss of activity | Glutamicibacter nicotianae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.3.6 | 0.07 | - |
octanoyl-CoA | - |
Glutamicibacter nicotianae | |
1.3.3.6 | 0.203 | - |
hexanoyl-CoA | - |
Glutamicibacter nicotianae | |
1.3.3.6 | 0.225 | - |
butyryl-CoA | - |
Glutamicibacter nicotianae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.3.6 | FAD | flavoprotein | Glutamicibacter nicotianae |