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Literature summary extracted from

  • Sztajer, H.; Wagner, G.; Schmid, R.D.
    Bacterial short-chain acyl-CoA oxidase: production, purification and characterization (1993), Appl. Microbiol. Biotechnol., 39, 708-713.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.3.6 MnCl2 slightly activating Glutamicibacter nicotianae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.6 AgNO3
-
Glutamicibacter nicotianae
1.3.3.6 HgCl2
-
Glutamicibacter nicotianae
1.3.3.6 p-chloromercuribenzoate slight inhibition Glutamicibacter nicotianae
1.3.3.6 Phenylmethylsulfonylfluoride slight inhibition Glutamicibacter nicotianae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.6 0.061
-
octanoyl-CoA
-
Glutamicibacter nicotianae
1.3.3.6 0.092
-
hexanoyl-CoA
-
Glutamicibacter nicotianae
1.3.3.6 0.1319
-
butyryl-CoA
-
Glutamicibacter nicotianae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.6 50000
-
2 * 50000, SDS-PAGE Glutamicibacter nicotianae
1.3.3.6 100000
-
gel filtration Glutamicibacter nicotianae

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.6 Glutamicibacter nicotianae
-
inducible
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.6
-
Glutamicibacter nicotianae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.6 77.14
-
purified enzyme Glutamicibacter nicotianae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.6 acyl-CoA + O2 highly specific for short-chain fatty acids Glutamicibacter nicotianae trans-2,3-dehydroacyl-CoA + H2O2
-
?
1.3.3.6 butyryl-CoA + O2
-
Glutamicibacter nicotianae trans-2-butenoyl-CoA + H2O2
-
?
1.3.3.6 hexanoyl-CoA + O2
-
Glutamicibacter nicotianae (2E)-hex-2-enoyl-CoA + H2O2
-
?
1.3.3.6 octanoyl-CoA + O2
-
Glutamicibacter nicotianae trans-2-octenoyl-CoA + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.3.3.6 dimer 2 * 50000, SDS-PAGE Glutamicibacter nicotianae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.6 additional information
-
FAD enhances thermal stability Glutamicibacter nicotianae
1.3.3.6 60
-
30 min, with butyryl-CoA and FAD, 50% loss of activity Glutamicibacter nicotianae
1.3.3.6 60
-
30 min, with butyryl-CoA, without FAD, 85% loss of activity Glutamicibacter nicotianae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.6 0.07
-
octanoyl-CoA
-
Glutamicibacter nicotianae
1.3.3.6 0.203
-
hexanoyl-CoA
-
Glutamicibacter nicotianae
1.3.3.6 0.225
-
butyryl-CoA
-
Glutamicibacter nicotianae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.6 FAD flavoprotein Glutamicibacter nicotianae