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Literature summary extracted from

  • Cardenas-Vazquez, R.; Yokosuka, O.; Billing, B.H.
    Enzymic oxidation of unconjugated bilirubin by rat liver (1986), Biochem. J., 236, 625-633.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.3.5 Salt maximum activity at a ionic strength of 60 mM, independent of salt type Rattus norvegicus
1.3.3.5 Triton X-100 0.01%, 25% increase in activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.5 albumin 0.008 mM, 50% inhibition Rattus norvegicus
1.3.3.5 Antimycin
-
 Rattus norvegicus
1.3.3.5 ATP
-
 Rattus norvegicus
1.3.3.5 bromosulphothalein 1 mM, 60% inhibition Rattus norvegicus
1.3.3.5 cysteine 1 mM, 40% inhibition Rattus norvegicus
1.3.3.5 Digitonin 0.1%, 50% inhibition Rattus norvegicus
1.3.3.5 dithiothreitol 1 mM, 60% inhibition Rattus norvegicus
1.3.3.5 KCN 0.03 mM, 70% inhibition Rattus norvegicus
1.3.3.5 mercaptoethanol 1 mM, 50% inhibition Rattus norvegicus
1.3.3.5 NADH 0.2 mM, 50% inhibition Rattus norvegicus
1.3.3.5 rotenone
-
 Rattus norvegicus
1.3.3.5 sodium taurocholate 0.5%, 50% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.5 0.136
-
 bilirubin
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.5 mitochondrion inner and outer membrane Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.5 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.5 0.00157
-
 enzyme activity in liver mitochondria Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 bilirubin + O2
-
 Rattus norvegicus biliverdin + H2O biliverdin + colourless diazo-negative compounds, including propentdyopents ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.5 40
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.3.5 10 70 10°C: about 36% of maximum, 70°C: about 10% of maximum Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.5 8.6
-
 Tris-HCl buffer Rattus norvegicus
1.3.3.5 8.9 9.2 diethylethanolamine buffer Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.5 8 10 at pH 8.0 and 10.0: about 30% of maximum activity Rattus norvegicus