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Literature summary extracted from

  • Tanaka, N.; Murao, S.
    Purification and some properties of bilirubin oxidase of Myrotheticum verrucaria MT-1 (1982), Agric. Biol. Chem., 46, 2499-2503.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.5 Fe2+ 1 mM, complete inhibition Albifimbria verrucaria
1.3.3.5 KCN 0.1 mM, complete inhibition Albifimbria verrucaria
1.3.3.5 Sodium azide 0.1 mM, 42% inhibition Albifimbria verrucaria
1.3.3.5 Thiourea 0.1 mM, 15% inhibition Albifimbria verrucaria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.5 0.19
-
 bilirubin
-
 Albifimbria verrucaria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.5 copper metalloenzyme, 1 atom of copper per enzyme molecule Albifimbria verrucaria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.5 52000
-
 gel filtration Albifimbria verrucaria

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.5 Albifimbria verrucaria
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.3.3.5 glycoprotein
-
 Albifimbria verrucaria

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.5 ammonium sulfate, charcoal, Sephadex a-50, Sephadex G-100 Albifimbria verrucaria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.5 culture medium
-
 Albifimbria verrucaria
-

Storage Stability

EC Number Storage Stability Organism
1.3.3.5 5°C, pH 9.2-9.7, 5 d Albifimbria verrucaria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.5 bilirubin + O2
-
 Albifimbria verrucaria biliverdin + H2O
-
 ?
1.3.3.5 biliverdin + O2 50% of activity with bilirubin Albifimbria verrucaria ?
-
 ?
1.3.3.5 catechol + O2 67% of activity with bilirubin Albifimbria verrucaria ?
-
 ?
1.3.3.5 chlorophyllin + O2 50% of activity with bilirubin Albifimbria verrucaria ?
-
 ?
1.3.3.5 hemin + O2 10% of activity with bilirubin Albifimbria verrucaria ?
-
 ?
1.3.3.5 hydroquinone + O2 20% of activity with bilirubin Albifimbria verrucaria ?
-
 ?
1.3.3.5 N,N-dimethyl-p-phenylenediamine + O2 23% of activity with bilirubin Albifimbria verrucaria ?
-
 ?
1.3.3.5 p-phenylenediamine + O2 15% of activity with bilirubin Albifimbria verrucaria ?
-
 ?
1.3.3.5 pyrogallol + O2 10% of activity with bilirubin Albifimbria verrucaria ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.5 40
-
-
 Albifimbria verrucaria

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.3.5 20 60 20°C: about 60% of maximum, 60°C: about 40% of maximum Albifimbria verrucaria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.3.5 37
-
 15 min, stable up to 37°C Albifimbria verrucaria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.5 8
-
-
 Albifimbria verrucaria

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.3.5 5 9.7 37°C, 60 min Albifimbria verrucaria
1.3.3.5 9.2 9.7
-
 Albifimbria verrucaria