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Literature summary extracted from

  • Watanabe, N.; Che, F.S.; Terashima, K.; Takayama, S.; Yoshida, S.; Isogai, A.
    Purification and properties of protoporphyrinogen oxidase from spinach chloroplasts (2000), Plant Cell Physiol., 41, 889-892.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.3.4 FAD
-
 Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.4 N-(4-chloro-2-fluoro-5-(propargyloxy)phenyl)-3,4,5,6-tetrahydrophthalimide
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.4 chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.4 60000
-
 SDS-PAGE, gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2 Spinacia oleracea penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4 from leaves Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.3.4 0.14
-
 purified enzyme Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Spinacia oleracea protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Spinacia oleracea protoporphyrin-IX + 3 H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.4 monomer
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.4 FAD
-
 Spinacia oleracea