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Literature summary extracted from

  • Dailey, H.A.; Dailey, T.A.
    Characteristics of human protoporphyrinogen oxidase in controls and variegate porphyrias (1997), Cell. Mol. Biol., 43, 67-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4 in Escherichia coli, His-tag inserted for purification Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.4 A433P reduced activity Homo sapiens
1.3.3.4 R168C reduced activity Homo sapiens
1.3.3.4 R59W reducued activity, lacks FAD-cofactor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.4 0.0017
-
 protoporphyrinogen-IX
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.4 51000
-
 calculated from amino acid sequence Homo sapiens
1.3.3.4 100000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2 Homo sapiens penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4 cloned enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.4 placenta
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
1.3.3.4 4°C, stable for days, slow proteolysis Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 mesoporphyrinogen-IX + 3 O2 100times lower activity Homo sapiens mesoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Homo sapiens protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Homo sapiens protoporphyrin-IX + 3 H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.4 dimer 2 * 51000 calculated MW, gel filtration Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.4 0.175
-
 protoporphyrinogen-IX
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.4 FAD one noncovalently bound FAD per dimer Homo sapiens