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Literature summary extracted from

  • Dailey, T.A.; Dailey, H.A.
    Human protoporphyrinogen oxidase: Expression, purification, and characterization of the cloned enzyme (1996), Protein Sci., 5, 98-105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4 in Escherichia coli, His-tag inserted for purification Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.3.4 acifluorfen
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.4 0.0017
-
 protoporphyrinogen-IX
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.4 51000
-
 SDS-PAGE Homo sapiens
1.3.3.4 100000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2 Homo sapiens penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4 cloned enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.4 placenta
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
1.3.3.4 4°C, stable for days, slow proteolysis Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen-IX + 3 O2
-
 Homo sapiens protoporphyrin-IX + 3 H2O2
-
 ?
1.3.3.4 protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Homo sapiens protoporphyrin-IX + 3 H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.4 dimer 2 * 51000, SDS-PAGE, gel filtration Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.4 0.175
-
 protoporphyrinogen-IX
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.4 FAD one noncovalently bound FAD per dimer Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.3.4 0.004
-
 acifluorfen
-
 Homo sapiens