BRENDA - Enzyme Database

Coproporphyrinogen oxidase. I. Purification, properties, and activation by phospholipids

Yoshinaga, T.; Sano, S.; J. Biol. Chem. 255, 4722-4726 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.3.3
2-mercaptoethanol
slight activation
Bos taurus
1.3.3.3
cardiolipin
2.2fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
DTT
slight activation
Bos taurus
1.3.3.3
L-alpha-lysophosphatidylcholine
4.4fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-lysophosphatidylethanolamine
3.4fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-phosphatidyl-DL-glycerol
2.7fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-phosphatidyl-L-serine
2.8fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-phosphatidylcholine
3.8fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-Phosphatidylethanolamine
3.9fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
Lipids
bovine liver lipids, 3.6fold activation at 1 mg/ml
Bos taurus
1.3.3.3
phosphatidate
2.3fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
Triton X-100
concentration-dependent activation up to 2.8fold
Bos taurus
1.3.3.3
Tween 20
2.4 fold activation at 0.2%, v/v
Bos taurus
1.3.3.3
Tween 40
2.2fold activation at 0.2%, v/v
Bos taurus
1.3.3.3
Tween 60
2.3fold activation at 0.2%, v/v
Bos taurus
1.3.3.3
Tween 80
2fold activation at 0.2%, v/v
Bos taurus
1.3.3.3
Tween 85
1.8 fold activation at 0.2%, v/v
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.3.3
cetyltrimethylammonium chloride
86% inhibition at 0.05%
Bos taurus
1.3.3.3
SDS
84% inhibition at 0.05%, w/v
Bos taurus
1.3.3.3
sodium deoxycholate
71% inhibition at 0.05%, w/v
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.3.3
0.018
0.025
Coproporphyrinogen-III
presence of phospholipids
Bos taurus
1.3.3.3
0.025
-
Coproporphyrinogen-III
crude extract
Bos taurus
1.3.3.3
0.048
-
Coproporphyrinogen-III
purified enzyme
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.3.3
additional information
no metal content
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.3.3
71600
-
1 * 71600, SDS-PAGE
Bos taurus
1.3.3.3
74000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.3.3
coproporphyrinogen-III + O2
Bos taurus
highly specific for oxygen as electron acceptor
protoporphyrinogen-IX + CO2
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.3.3
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.3.3.3
to homogeneity, chromatography steps
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.3.3
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.3.3
0.12
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
1.3.3.3
4°C, 0.1 M Tris-HCl, pH 7.4, 50% saturation ammonium sulfate, 2 weeks
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.3.3
coproporphyrinogen-III + O2
highly specific for oxygen as electron acceptor
390964
Bos taurus
protoporphyrinogen-IX + CO2
-
390964
Bos taurus
?
Subunits
EC Number
Subunits
Commentary
Organism
1.3.3.3
monomer
1 * 71600, SDS-PAGE
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.3.3
7.4
-
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.3.3
6.4
8.4
80% of the maximum activity within
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.3.3.3
2-mercaptoethanol
slight activation
Bos taurus
1.3.3.3
cardiolipin
2.2fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
DTT
slight activation
Bos taurus
1.3.3.3
L-alpha-lysophosphatidylcholine
4.4fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-lysophosphatidylethanolamine
3.4fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-phosphatidyl-DL-glycerol
2.7fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-phosphatidyl-L-serine
2.8fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-phosphatidylcholine
3.8fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
L-alpha-Phosphatidylethanolamine
3.9fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
Lipids
bovine liver lipids, 3.6fold activation at 1 mg/ml
Bos taurus
1.3.3.3
phosphatidate
2.3fold activation at 0.5 mg/ml
Bos taurus
1.3.3.3
Triton X-100
concentration-dependent activation up to 2.8fold
Bos taurus
1.3.3.3
Tween 20
2.4 fold activation at 0.2%, v/v
Bos taurus
1.3.3.3
Tween 40
2.2fold activation at 0.2%, v/v
Bos taurus
1.3.3.3
Tween 60
2.3fold activation at 0.2%, v/v
Bos taurus
1.3.3.3
Tween 80
2fold activation at 0.2%, v/v
Bos taurus
1.3.3.3
Tween 85
1.8 fold activation at 0.2%, v/v
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.3.3.3
cetyltrimethylammonium chloride
86% inhibition at 0.05%
Bos taurus
1.3.3.3
SDS
84% inhibition at 0.05%, w/v
Bos taurus
1.3.3.3
sodium deoxycholate
71% inhibition at 0.05%, w/v
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.3.3.3
0.018
0.025
Coproporphyrinogen-III
presence of phospholipids
Bos taurus
1.3.3.3
0.025
-
Coproporphyrinogen-III
crude extract
Bos taurus
1.3.3.3
0.048
-
Coproporphyrinogen-III
purified enzyme
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.3.3.3
additional information
no metal content
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.3.3.3
71600
-
1 * 71600, SDS-PAGE
Bos taurus
1.3.3.3
74000
-
gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.3.3
coproporphyrinogen-III + O2
Bos taurus
highly specific for oxygen as electron acceptor
protoporphyrinogen-IX + CO2
-
Bos taurus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.3.3
to homogeneity, chromatography steps
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.3.3
liver
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.3.3.3
0.12
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.3.3.3
4°C, 0.1 M Tris-HCl, pH 7.4, 50% saturation ammonium sulfate, 2 weeks
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.3.3
coproporphyrinogen-III + O2
highly specific for oxygen as electron acceptor
390964
Bos taurus
protoporphyrinogen-IX + CO2
-
390964
Bos taurus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.3.3.3
monomer
1 * 71600, SDS-PAGE
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.3.3.3
7.4
-
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.3.3.3
6.4
8.4
80% of the maximum activity within
Bos taurus